Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Thiowin_RS10395 Genome accession   NZ_CP121472
Coordinates   2343205..2344917 (+) Length   570 a.a.
NCBI ID   WP_328987661.1    Uniprot ID   -
Organism   Thiorhodovibrio winogradskyi strain DSM 6702     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2338205..2349917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiowin_RS10380 (Thiowin_02134) dnaJ 2338484..2339632 (+) 1149 WP_328987658.1 molecular chaperone DnaJ -
  Thiowin_RS10385 (Thiowin_02135) acs 2340071..2342017 (+) 1947 WP_328987659.1 acetate--CoA ligase -
  Thiowin_RS10390 (Thiowin_02136) - 2342155..2342997 (+) 843 WP_328987660.1 NYN domain-containing protein -
  Thiowin_RS10395 (Thiowin_02137) pilB 2343205..2344917 (+) 1713 WP_328987661.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Thiowin_RS10400 (Thiowin_02138) pilC 2344923..2346176 (+) 1254 WP_328987662.1 type II secretion system F family protein Machinery gene
  Thiowin_RS10405 (Thiowin_02139) pilD 2346222..2347133 (+) 912 WP_328987663.1 A24 family peptidase Machinery gene
  Thiowin_RS10410 (Thiowin_02140) coaE 2347134..2347742 (+) 609 WP_328987664.1 dephospho-CoA kinase -
  Thiowin_RS10415 (Thiowin_02141) - 2347832..2349250 (-) 1419 WP_328987665.1 PhoH family protein -
  Thiowin_RS10420 (Thiowin_02142) - 2349439..2349903 (-) 465 WP_328987666.1 peroxiredoxin -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62525.36 Da        Isoelectric Point: 5.0261

>NTDB_id=812319 Thiowin_RS10395 WP_328987661.1 2343205..2344917(+) (pilB) [Thiorhodovibrio winogradskyi strain DSM 6702]
MATNQSEVNLSGLARRLVRDELISEEAARKIQEEASRRREPFVQCLVAQKALDSQVVALAASQEFGVPVLDLSAMDLQSL
PLSLVDERLITKHRALPLFRRGNRLFLAISDPTNDQALDEIRFNTGLATDSVLVEEDKLAAALIKAVEGSGAGGVDVSDA
DLENIDVQDTDSEQRDDGDANVDDAPIVRYVNKILVDAISSGTSDIHFEPYEKSCRVRFRQDGLLHEVANPPLSIAGRLI
SRLKVMSRMNIAERRVPQDGRIKLKLSRTRDIDFRVNTLPTLYGEKVVLRILDSSAANVGIESLGMDEKQRKDFMSSIDK
PYGMILVTGPTGSGKTVTLYSALNILNKPEINISTVEDPVEIQVPGINQVNLNPKSGLTFAEALRAFLRQDPDIVMVGEI
RDLETAEIAVKAAQTGHLVLSTLHTNDAPQSLTRLANMGVPPFNIASSVLLIMAQRLARKLCPVCRQAEHIPAEALLEEG
FTQEEVDEGVTIYRPVGCNQCNNGYKGRVGIFQIIKVSEEMGRLIMEGGNSIQLAKQAKTEGYADLRQSGLRKVKAGVTS
LEELNRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=812319 Thiowin_RS10395 WP_328987661.1 2343205..2344917(+) (pilB) [Thiorhodovibrio winogradskyi strain DSM 6702]
ATGGCGACCAATCAGTCGGAAGTCAATCTGTCAGGCCTCGCGCGCAGGCTCGTCCGTGACGAGCTGATCTCCGAGGAGGC
AGCGCGCAAAATCCAAGAAGAGGCATCGCGCCGTCGCGAGCCTTTTGTGCAGTGTTTGGTTGCACAGAAGGCGTTGGATA
GTCAAGTTGTCGCACTGGCGGCCTCTCAGGAGTTCGGTGTCCCCGTGCTTGACCTGAGCGCGATGGATTTGCAGTCGCTG
CCGCTGTCCCTGGTCGATGAACGCTTGATTACCAAACATCGAGCTCTGCCGCTATTTCGTCGCGGCAACCGGCTGTTTCT
GGCAATTTCCGATCCCACCAATGACCAAGCCCTGGATGAAATTCGCTTTAACACGGGTCTGGCAACCGACTCGGTGCTGG
TGGAAGAAGACAAGCTTGCGGCAGCCCTGATCAAAGCGGTGGAGGGTTCCGGTGCTGGCGGGGTGGATGTCTCGGATGCC
GATCTTGAAAATATTGATGTCCAGGATACGGACAGCGAACAACGCGACGATGGCGACGCTAACGTCGATGATGCGCCCAT
TGTGCGGTATGTGAATAAAATTCTGGTCGACGCCATCTCCAGTGGCACTTCGGATATTCACTTCGAACCCTACGAGAAAA
GCTGTCGTGTGCGTTTTCGCCAGGATGGCCTGCTGCACGAGGTTGCCAATCCTCCGCTGAGCATCGCTGGTCGCCTGATA
TCGCGACTCAAGGTCATGTCGCGCATGAATATTGCCGAGCGGCGCGTGCCCCAGGATGGGCGGATCAAGCTCAAGCTGTC
GCGCACCCGCGACATAGATTTTCGTGTCAATACCTTGCCGACGCTCTATGGCGAGAAAGTAGTGCTGCGTATCCTCGATT
CCTCCGCCGCCAATGTCGGGATCGAAAGCCTTGGCATGGATGAAAAGCAACGCAAGGACTTCATGTCGAGTATCGACAAG
CCCTATGGCATGATCCTGGTGACAGGGCCAACCGGCTCGGGCAAGACGGTGACGCTCTATTCGGCGCTTAATATTTTGAA
CAAGCCGGAAATCAATATTTCCACCGTTGAGGATCCGGTCGAGATTCAGGTGCCCGGCATTAACCAGGTCAACTTGAATC
CTAAAAGCGGTCTGACCTTCGCCGAGGCCCTGCGGGCCTTCCTGCGCCAGGATCCGGATATCGTCATGGTCGGTGAGATT
CGTGACCTTGAAACCGCCGAGATTGCCGTCAAGGCGGCTCAGACTGGCCATTTGGTGCTCTCCACCCTGCATACCAACGA
TGCACCCCAATCCCTGACTCGTTTGGCCAACATGGGTGTTCCGCCTTTTAATATTGCCTCTTCCGTGCTGCTCATTATGG
CGCAACGGCTGGCGCGCAAGCTCTGTCCGGTGTGCCGGCAAGCAGAGCATATTCCAGCCGAGGCGCTGCTTGAGGAGGGC
TTTACCCAAGAGGAAGTTGACGAAGGTGTGACCATTTACCGTCCCGTTGGCTGCAATCAATGCAACAATGGATATAAAGG
TCGCGTTGGAATTTTTCAGATCATCAAGGTCTCGGAGGAAATGGGGCGGCTGATCATGGAAGGGGGGAATTCCATTCAGC
TTGCCAAACAGGCCAAGACCGAAGGCTATGCGGATCTCCGCCAGTCGGGTCTGCGCAAGGTCAAGGCCGGGGTCACCAGT
CTTGAAGAGCTCAACCGGGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.783

100

0.553
  pilB Acinetobacter baumannii D1279779

54.101

100

0.544
  pilB Acinetobacter baylyi ADP1

52.715

100

0.528
  pilB Vibrio cholerae strain A1552

48.932

98.596

0.482
  pilB Vibrio campbellii strain DS40M4

49.907

94.561

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

49.536

94.561

0.468
  pilF Neisseria gonorrhoeae MS11

47.331

98.596

0.467
  pilF Thermus thermophilus HB27

39.122

95.965

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.509

0.374