Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSm6_RS07825 Genome accession   NZ_AP023081
Coordinates   1693677..1695755 (-) Length   692 a.a.
NCBI ID   WP_265169989.1    Uniprot ID   -
Organism   Pseudomonas solani strain Sm006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1688677..1700755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSm6_RS07810 (PSm6_15700) - 1690308..1691987 (-) 1680 WP_265169986.1 SPOR domain-containing protein -
  PSm6_RS07815 (PSm6_15710) aroB 1691998..1693101 (-) 1104 WP_265169987.1 3-dehydroquinate synthase -
  PSm6_RS07820 (PSm6_15720) aroK 1693153..1693671 (-) 519 WP_265169988.1 shikimate kinase AroK -
  PSm6_RS07825 (PSm6_15730) pilQ 1693677..1695755 (-) 2079 WP_265169989.1 type IV pilus secretin PilQ Machinery gene
  PSm6_RS07830 (PSm6_15740) pilP 1695809..1696342 (-) 534 WP_265169990.1 type 4a pilus biogenesis lipoprotein PilP -
  PSm6_RS07835 (PSm6_15750) pilO 1696339..1696962 (-) 624 WP_265169992.1 type 4a pilus biogenesis protein PilO -
  PSm6_RS07840 (PSm6_15760) pilN 1696959..1697531 (-) 573 WP_265169993.1 type 4a pilus biogenesis protein PilN -
  PSm6_RS07845 (PSm6_15770) pilM 1697531..1698595 (-) 1065 WP_021221345.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 73996.10 Da        Isoelectric Point: 4.8234

>NTDB_id=81201 PSm6_RS07825 WP_265169989.1 1693677..1695755(-) (pilQ) [Pseudomonas solani strain Sm006]
MNSSLSRLGISLLAAMLSPVLLAADLQGLDVAALPGGKVELKLAFDEPIVAPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTIVEAKDRTRLIVNLATLVPYSTRVEGNNLFVIVGEAAVAGQQVASSAPVAAPVAAAPVKTFAAAGKAISN
IDFQRGDQGEGNVVITLSDPTVSPDIQEQGGKIRLDFSKTQLPESLRVRLDVKDFATPVQFVSASGSSGNASITIEPTGY
YDYLAYQTENKLTVSIKPLTQDDVEKRKNERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTSGDPS
ASGADDRGSITVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVNWRGARVGDNNFVIGG
AGAGRPTATTPTGALGTFVDMGADGATSSLGIGFVTDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGAEIPY
QEASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSNALNGVPPINKNEVNAKVLVSDGETIVIGGVFSN
TQTKATDKVPFLGDLPFLGRMFKRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=81201 PSm6_RS07825 WP_265169989.1 1693677..1695755(-) (pilQ) [Pseudomonas solani strain Sm006]
ATGAACAGCTCGCTTTCGCGCCTCGGTATTTCTCTATTGGCCGCCATGTTGTCGCCAGTACTGCTGGCCGCTGACCTGCA
AGGCCTGGATGTAGCGGCTTTGCCTGGTGGCAAGGTTGAGCTGAAACTCGCCTTTGATGAGCCCATTGTGGCGCCGCGGG
GGTACACCATCGAGCAGCCGGCGCGCATCGCTCTGGATCTTCCTGGAGTGGCCAACAAGCTGGGCAGCAAGAACCGTGAG
CTCGGCGTTGGTAACGCGCGCAGCGTCACTATCGTTGAAGCGAAAGACCGTACTCGGTTGATCGTCAACCTGGCGACACT
CGTGCCTTACAGCACGCGTGTTGAGGGTAACAACCTCTTCGTCATCGTCGGTGAGGCAGCTGTCGCCGGCCAGCAAGTTG
CCAGTTCCGCTCCTGTGGCGGCACCTGTCGCAGCTGCGCCCGTCAAGACTTTTGCGGCGGCCGGCAAGGCGATCAGCAAT
ATCGACTTCCAGCGTGGCGATCAGGGCGAGGGTAATGTTGTCATCACTCTGTCCGACCCGACTGTGAGCCCGGATATTCA
GGAGCAGGGTGGCAAGATCCGCCTCGACTTTTCCAAGACCCAGCTTCCTGAGTCTCTGCGTGTCCGTCTTGATGTGAAGG
ATTTCGCGACCCCTGTTCAATTCGTCAGTGCCTCTGGCTCTTCGGGGAATGCGAGTATCACCATCGAGCCCACTGGCTAT
TACGACTACCTGGCGTATCAGACCGAGAACAAGCTCACCGTCAGCATCAAGCCGTTGACGCAGGATGATGTCGAGAAGCG
CAAGAACGAACGCTTTGCCTACTCCGGTGAGAAGCTCTCGCTGAACTTCCAGGACATCGATGTGCGTTCGGTGCTGCAAT
TGATTGCCGACTTCACCGACCTCAACCTGGTGGCCTCGGATACCGTTCAAGGCAATATCACGCTGCGTCTGCAGAACGTA
CCTTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGCCTTGATAAGCGCAAGGTTGGTAATGTTCTGCTGGTTGC
ACCGGCAGATGAGATCGCTGCACGTGAGCGCCAAGAGTTGGAATCGCAGAAGCAAATTGCTGAGCTCGCTCCGTTGCGCC
GTGAACTCATCCAAGTCAACTATGCCAAGGCGGCTGATATTGCCAAGCTGTTCCAGTCGGTCACCAGTGGCGATCCCAGC
GCAAGTGGTGCTGATGATCGGGGCTCCATTACCGTGGATGATCGTACCAACAGCATCATCGCTTACCAGACTCAGGACAA
GCTGGACGAACTGCGCCGTATCGTTGCGCAACTCGACATCCCGGTTCGTCAGGTCATGATCGAGGCCCGTATCGTCGAAG
CCAACGTGGACTACGACAAGTCGCTTGGTGTGAACTGGCGAGGTGCTCGTGTTGGTGACAATAACTTCGTCATTGGTGGC
GCAGGCGCAGGCCGCCCGACTGCTACTACGCCTACCGGCGCACTGGGGACATTCGTCGATATGGGGGCGGATGGGGCCAC
TTCTTCCCTCGGCATCGGCTTCGTAACCGACAACACCATCCTTGACCTCGAACTCTCCGCCATGGAGAAGACCGGTAACG
GCGAGATCGTGTCCCAGCCCAAGGTCGTTACCTCGGACAAGGAAACCGCCAAGATCCTGAAGGGCGCTGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGTGCCAGCACCACCTCCTTCAAGGAAGCGGCGCTGTCCCTGGAAGTGACCCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAAGTCAAGGTGACCAAGGACGCCCCGGACTTCTCCAACGCGCTGAATGGCGTACCGC
CGATCAACAAGAACGAGGTCAACGCCAAGGTTCTGGTCAGCGATGGCGAAACCATCGTGATCGGTGGTGTGTTCTCCAAC
ACCCAGACCAAGGCGACCGACAAGGTTCCCTTCCTCGGTGACCTGCCGTTCCTAGGTCGCATGTTCAAGCGTGATGTGGT
CCAGGACAAGAAATCCGAGCTGCTGGTCTTCCTCACCCCGCGTATCATGAACAACCAGGCTATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(40-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.923

100

0.795
  comQ Acinetobacter baylyi ADP1

42.899

99.711

0.428
  pilQ Acinetobacter baumannii strain A118

40.799

100

0.413
  pilQ Acinetobacter baumannii D1279779

40.799

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

38.067

98.699

0.376
  pilQ Legionella pneumophila strain Lp02

38.067

98.699

0.376

Multiple sequence alignment