Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PSm6_RS07845 Genome accession   NZ_AP023081
Coordinates   1697531..1698595 (-) Length   354 a.a.
NCBI ID   WP_021221345.1    Uniprot ID   A0AAU7Y112
Organism   Pseudomonas solani strain Sm006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1692531..1703595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSm6_RS07820 (PSm6_15720) aroK 1693153..1693671 (-) 519 WP_265169988.1 shikimate kinase AroK -
  PSm6_RS07825 (PSm6_15730) pilQ 1693677..1695755 (-) 2079 WP_265169989.1 type IV pilus secretin PilQ Machinery gene
  PSm6_RS07830 (PSm6_15740) pilP 1695809..1696342 (-) 534 WP_265169990.1 type 4a pilus biogenesis lipoprotein PilP -
  PSm6_RS07835 (PSm6_15750) pilO 1696339..1696962 (-) 624 WP_265169992.1 type 4a pilus biogenesis protein PilO -
  PSm6_RS07840 (PSm6_15760) pilN 1696959..1697531 (-) 573 WP_265169993.1 type 4a pilus biogenesis protein PilN -
  PSm6_RS07845 (PSm6_15770) pilM 1697531..1698595 (-) 1065 WP_021221345.1 pilus assembly protein PilM Machinery gene
  PSm6_RS07850 (PSm6_15780) - 1698780..1701200 (+) 2421 WP_162164735.1 penicillin-binding protein 1A -
  PSm6_RS07855 (PSm6_15790) - 1701316..1702584 (-) 1269 WP_021221347.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37767.98 Da        Isoelectric Point: 4.4899

>NTDB_id=81202 PSm6_RS07845 WP_021221345.1 1697531..1698595(-) (pilM) [Pseudomonas solani strain Sm006]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLSKAKASSKSVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSSRNSERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAAQLGSGHDELTVAVVDIGATMTTLSVLHNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=81202 PSm6_RS07845 WP_021221345.1 1697531..1698595(-) (pilM) [Pseudomonas solani strain Sm006]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCACCGAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGTTGTCCCGTGTTCTGAGCAAGGCGAAAGCCAGCTCCAAGTCCGTGGCTGTT
GCCGTGGCGGGCTCCGCTGTAATCACCAAGACCATCGAGATGGATGCCGGTCTTTCCGACGACGAGTTGGAAAACCAGCT
GAAGATCGAGGCGGACCAGTACATCCCTTACCCGCTCGAAGAAGTCGCCATCGACTTCGAGGTGCAGGGTGTGTCTTCGC
GCAACTCCGAGCGGGTTGATGTGCTGCTTGCGGCATGCCGCAAGGAGAACGTCGAGGTCCGTGAGGCGGCGCTGGCACTG
GCGGGTCTGACCGCCAAGGTCGTCGACGTCGAAGCCTATGCGTTGGAACGTGCCTATGGGCTGCTCGCTGCGCAACTGGG
CAGCGGCCATGACGAGCTGACCGTCGCAGTGGTGGATATCGGCGCGACCATGACGACGCTGAGCGTTCTGCATAACGGGC
GGACCATTTACAGCCGCGAGCAGCTTTTCGGTGGGCGTCAGCTCACCGAGGAGATTCAGCGCCGCTATGGCCTTTCCGTG
GAAGAGGCTGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAGCCGTTCAAGGATGC
AGTGGTCCAGCAGGTGTCTCGCTCGCTGCAGTTCTTCTTCGCTGCGGGTCAGTTCAACGATGTCGACTACATCCTCCTGG
CGGGCGGTACTGCGTCCATTCCTGATCTGGATCGGCTGATCCAGCAGAAGATCGGCACTCCTACCCAGGTTGCCAACCCC
TTCGCGGAGATGGCGCTGAGCGGCAAGGTCAATGCGGGTGCATTGGCCAGTGATGCCCCGGCGCTGATGATTGCCTGCGG
ACTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

48.451

100

0.486

Multiple sequence alignment