Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RS171_RS11340 Genome accession   NZ_CP135649
Coordinates   2230294..2231679 (+) Length   461 a.a.
NCBI ID   WP_001025193.1    Uniprot ID   -
Organism   Escherichia coli strain TUM14664     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2225294..2236679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS171_RS11315 (RS171_11315) aroP 2225845..2227218 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  RS171_RS11320 (RS171_11320) ampE 2227261..2228115 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RS171_RS11325 (RS171_11325) ampD 2228112..2228663 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RS171_RS11330 (RS171_11330) nadC 2228751..2229644 (+) 894 WP_001514911.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RS171_RS11335 (RS171_11335) ppdD 2229844..2230284 (+) 441 WP_032277822.1 prepilin peptidase-dependent pilin -
  RS171_RS11340 (RS171_11340) pilB 2230294..2231679 (+) 1386 WP_001025193.1 type II secretion system protein GspE Machinery gene
  RS171_RS11345 (RS171_11345) hofC 2231669..2232871 (+) 1203 WP_032277820.1 protein transport protein HofC -
  RS171_RS11350 (RS171_11350) guaC 2232906..2233949 (-) 1044 WP_001217338.1 GMP reductase -
  RS171_RS11355 (RS171_11355) - 2234105..2234149 (-) 45 WP_120795372.1 protein YacM -
  RS171_RS11360 (RS171_11360) coaE 2234174..2234794 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  RS171_RS11365 (RS171_11365) zapD 2234794..2235537 (+) 744 WP_001194722.1 cell division protein ZapD -
  RS171_RS11370 (RS171_11370) yacG 2235547..2235744 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  RS171_RS11375 (RS171_11375) mutT 2235960..2236349 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50623.30 Da        Isoelectric Point: 6.5396

>NTDB_id=811035 RS171_RS11340 WP_001025193.1 2230294..2231679(+) (pilB) [Escherichia coli strain TUM14664]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCIHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=811035 RS171_RS11340 WP_001025193.1 2230294..2231679(+) (pilB) [Escherichia coli strain TUM14664]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCACCGCTGCCTCACTGGCAGGCACCCGGTTGTATACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36