Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   P6910_RS06460 Genome accession   NZ_CP120717
Coordinates   1772569..1774650 (+) Length   693 a.a.
NCBI ID   WP_317145456.1    Uniprot ID   -
Organism   Endozoicomonas sp. 8E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1767569..1779650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6910_RS06440 (P6910_06435) comM 1769534..1770598 (+) 1065 WP_317145452.1 pilus assembly protein PilM Machinery gene
  P6910_RS06445 (P6910_06440) - 1770598..1771164 (+) 567 WP_317145453.1 PilN domain-containing protein -
  P6910_RS06450 (P6910_06445) - 1771161..1771775 (+) 615 WP_317145454.1 type 4a pilus biogenesis protein PilO -
  P6910_RS06455 (P6910_06450) - 1771775..1772302 (+) 528 WP_317145455.1 pilus assembly protein PilP -
  P6910_RS06460 (P6910_06455) pilQ 1772569..1774650 (+) 2082 WP_317145456.1 type IV pilus secretin PilQ family protein Machinery gene
  P6910_RS06465 (P6910_06460) - 1774778..1775137 (+) 360 Protein_1281 DUF29 domain-containing protein -
  P6910_RS06470 (P6910_06465) aroK 1775358..1775876 (+) 519 WP_317145457.1 shikimate kinase AroK -
  P6910_RS06475 (P6910_06470) aroB 1775958..1777049 (+) 1092 WP_317146513.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 74916.18 Da        Isoelectric Point: 4.5614

>NTDB_id=808237 P6910_RS06460 WP_317145456.1 1772569..1774650(+) (pilQ) [Endozoicomonas sp. 8E]
MNNKPVLKASAVRMLLYMVLAWLPLSVWGITLKKMDAGTLPGDIVELRLMFDGPAPEAKGYTIEQPPRISLDLPFTRSDL
PKYNEVGFQNTESVTVLEAGGRTRLVVNLKTAAGFTTKADGNVLYVYVGAEGSNTRSVLPTVASSGAPLADPASQGITHI
DFQRGEEGEGNVVITLADPNIPMDMNELGGRIRLEFQGNVLPGRLRNRLDVIDFATPVKFIDAKVEDGNAVVVIEPKGDY
SYLAYQADNTLTVSVKPVSDKDYGRDRKGLAYKGDKLSLNFQDIEVRAVLQLIADFTDLNLVASDTVSGNVTLRLQNVPW
DQALDIVLKSKGLDKRLEGNVLTVAPAEEIAARERQQLENDKQIRELAPVYTDLIQINYADAEEIATVLAGEGEETSLLT
ERGSVQVVARTNSLLVKDTQEKLDELRALIQRLDIPVRQVMIEARIVNMNSNFTRDLGVKWGGATDFDRSANRDVAVGGQ
GTQATIVDGVPTVQLPDNAPFLDFGVAGENAATLAIGFATNSTLLNLELSAILSDGGGETVSQPKVITADKTKAVIKSGK
EIPYEEKTSSGATSVAFKDAVLSLEVTPQITPEGSIIMDVTVTNDSQGADAPNGVPTINKNEVNTQVLVRDGETVVLGGV
FTQEKKKGAVKVPVLGDIPYVGRLFRKDTNSDVKTELMIFITPRVVNDNITLR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=808237 P6910_RS06460 WP_317145456.1 1772569..1774650(+) (pilQ) [Endozoicomonas sp. 8E]
ATGAATAATAAGCCCGTGTTGAAAGCCAGCGCAGTACGAATGCTGCTGTATATGGTGTTGGCGTGGTTGCCGCTTTCTGT
CTGGGGTATAACACTCAAGAAAATGGATGCTGGCACCCTGCCGGGTGATATCGTAGAGCTGCGTCTGATGTTCGATGGCC
CTGCACCTGAGGCCAAGGGGTACACGATAGAGCAGCCTCCCAGGATTTCTTTGGATTTACCGTTCACTCGCAGCGATCTG
CCCAAATATAACGAAGTTGGATTTCAGAACACAGAGAGTGTCACAGTGCTTGAGGCTGGGGGCAGAACCCGGCTTGTTGT
TAATTTGAAAACAGCCGCCGGTTTTACCACTAAAGCAGACGGTAATGTGCTCTATGTTTATGTGGGCGCAGAAGGTTCTA
ATACTCGTTCAGTTCTTCCAACAGTAGCCAGTTCTGGTGCGCCTTTGGCTGATCCTGCCAGCCAGGGTATTACCCATATT
GATTTCCAGAGGGGGGAAGAAGGAGAGGGTAATGTTGTTATTACGCTCGCAGATCCCAACATTCCAATGGATATGAATGA
GCTGGGTGGTCGCATTCGTCTTGAGTTTCAGGGGAATGTTCTGCCAGGCCGGTTACGTAACCGTTTGGACGTCATTGATT
TCGCTACTCCCGTAAAATTCATTGATGCCAAGGTCGAGGACGGGAATGCAGTGGTCGTTATCGAGCCCAAGGGGGATTAC
AGCTACCTTGCTTATCAGGCAGATAACACACTGACAGTCAGTGTTAAGCCGGTATCCGATAAAGATTATGGTCGTGACCG
GAAAGGTCTGGCCTATAAGGGAGACAAGCTGTCCCTCAACTTTCAGGATATCGAAGTGCGCGCTGTTCTTCAGTTGATTG
CTGACTTTACCGATCTGAACCTGGTGGCCTCAGATACGGTCAGCGGTAACGTGACACTGCGTCTGCAGAATGTGCCCTGG
GATCAGGCTCTGGACATCGTTCTCAAATCAAAAGGTCTGGACAAGCGACTTGAAGGTAATGTCCTGACTGTGGCTCCAGC
CGAAGAGATTGCAGCCAGAGAGCGTCAGCAACTGGAGAATGACAAGCAGATTCGTGAGCTGGCGCCTGTTTATACCGATC
TGATTCAGATTAATTACGCAGATGCAGAAGAGATTGCGACGGTTCTTGCCGGTGAAGGCGAAGAGACCAGTCTGCTGACT
GAGCGTGGTTCGGTTCAGGTGGTGGCTCGTACTAACAGTCTTCTGGTTAAGGATACCCAGGAAAAGCTGGATGAACTGCG
TGCGCTGATTCAACGTCTCGATATCCCTGTCAGACAGGTGATGATCGAGGCGAGGATTGTCAATATGAACTCCAACTTCA
CCCGTGATCTGGGGGTAAAGTGGGGAGGCGCGACAGACTTTGATCGTTCAGCCAATCGGGATGTAGCTGTCGGTGGTCAG
GGGACTCAGGCGACTATCGTCGATGGTGTGCCAACTGTTCAATTGCCAGACAACGCTCCCTTTCTAGATTTTGGAGTGGC
TGGAGAAAACGCTGCAACGTTGGCGATTGGTTTTGCAACCAACAGTACTCTGCTGAATCTTGAGCTTTCAGCCATTCTGT
CAGATGGTGGTGGTGAAACGGTTTCTCAGCCAAAAGTGATAACCGCTGACAAGACTAAAGCAGTGATCAAGTCGGGTAAG
GAAATTCCTTACGAAGAGAAAACATCCAGTGGTGCGACCTCTGTAGCTTTCAAGGATGCGGTTCTGTCTCTCGAAGTGAC
TCCGCAGATCACGCCAGAGGGCAGTATTATTATGGATGTGACGGTTACCAATGACTCTCAGGGTGCTGACGCGCCTAATG
GTGTTCCTACCATCAATAAGAATGAAGTGAATACTCAGGTGTTGGTCAGAGATGGTGAAACAGTTGTTCTGGGTGGAGTA
TTTACTCAGGAGAAAAAGAAAGGGGCAGTTAAGGTGCCAGTGTTGGGCGATATTCCTTATGTTGGAAGATTGTTTAGAAA
AGACACCAATTCAGATGTCAAAACCGAGCTGATGATTTTCATTACACCCAGAGTGGTCAACGATAATATTACCCTGCGTT
AA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Bacterial type II and III secretion system protein (Secretin)

(534-686)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

52.199

100

0.531
  comQ Acinetobacter baylyi ADP1

44.2

98.268

0.434
  pilQ Acinetobacter baumannii D1279779

42.775

100

0.431
  pilQ Acinetobacter baumannii strain A118

42.632

100

0.43
  pilQ Legionella pneumophila strain Lp02

37.612

96.681

0.364
  pilQ Legionella pneumophila strain ERS1305867

37.651

95.815

0.361