Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P3T65_RS10980 Genome accession   NZ_CP120376
Coordinates   2362103..2363803 (-) Length   566 a.a.
NCBI ID   WP_024765633.1    Uniprot ID   A0A6G6IRV2
Organism   Pseudomonas nitroreducens strain L4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2357103..2368803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T65_RS10945 (P3T65_10945) - 2357511..2357945 (+) 435 WP_037007364.1 hypothetical protein -
  P3T65_RS10950 (P3T65_10950) - 2357942..2358631 (+) 690 WP_024765638.1 energy-coupling factor ABC transporter permease -
  P3T65_RS10955 (P3T65_10955) - 2358738..2358953 (+) 216 WP_024765637.1 hypothetical protein -
  P3T65_RS10960 (P3T65_10960) yacG 2359130..2359336 (-) 207 WP_024765636.1 DNA gyrase inhibitor YacG -
  P3T65_RS10965 (P3T65_10965) coaE 2359333..2359941 (-) 609 WP_024765635.1 dephospho-CoA kinase -
  P3T65_RS10970 (P3T65_10970) pilD 2360006..2360878 (-) 873 WP_038803226.1 prepilin peptidase Machinery gene
  P3T65_RS10975 (P3T65_10975) pilC 2360882..2362099 (-) 1218 WP_024765634.1 type II secretion system F family protein Machinery gene
  P3T65_RS10980 (P3T65_10980) pilB 2362103..2363803 (-) 1701 WP_024765633.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P3T65_RS10985 (P3T65_10985) - 2364030..2364566 (+) 537 WP_024765632.1 pilin -
  P3T65_RS10990 (P3T65_10990) tfpZ 2364662..2365384 (+) 723 WP_235376362.1 TfpX/TfpZ family type IV pilin accessory protein -
  P3T65_RS11000 (P3T65_11000) galE 2365587..2366603 (-) 1017 WP_038803227.1 UDP-glucose 4-epimerase GalE -
  P3T65_RS11005 (P3T65_11005) nadC 2366704..2367552 (-) 849 WP_277369277.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62358.35 Da        Isoelectric Point: 5.5929

>NTDB_id=805270 P3T65_RS10980 WP_024765633.1 2362103..2363803(-) (pilB) [Pseudomonas nitroreducens strain L4]
MNEQVVLSGLARQLVISELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAFCDLSSLDKEGFPKE
LVSEKLVRQHRVLPLWRRGNKLFVGVSDPTNHQAITDVQFSTGLTTEAILVEDDKLGEAIEKLFESVTGGLDDLGDIDLD
GLDIENGNTEAKEDAGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGMLHEVAKPPIQLATRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTMPTLWGEKIVMRILDSASAQLGIDALGYEDAQKQLYLEALKQPQG
MILVTGPTGSGKTVSLYTGINILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVAAFNLATSVNLIIAQRLARKLCPHCKKEHDVPAEALRHEGFPE
DKIGTFKLYSPVGCDNCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=805270 P3T65_RS10980 WP_024765633.1 2362103..2363803(-) (pilB) [Pseudomonas nitroreducens strain L4]
ATGAACGAACAAGTCGTCCTCTCCGGCCTCGCCCGGCAACTGGTGATTTCCGAGCTGCTCGACGAGAAGAGCGCCCAGCA
GGCCCAGCAACAGGCCCAACGCAACAAGTTGTCGCTGGTCACCTACCTGGTGCAGAACAAGTTGGTGAAGAGCCGGCCAT
TGACAGAGCTGGCTGCAGATCAATTCGGTGTGGCGTTCTGCGACCTGTCCAGCCTGGACAAGGAAGGCTTTCCAAAAGAG
CTGGTCAGCGAGAAGCTGGTGCGCCAGCACCGCGTGCTGCCGCTCTGGCGCCGGGGCAACAAGCTATTCGTGGGGGTCTC
CGACCCGACCAATCACCAGGCGATCACCGATGTCCAGTTCAGTACCGGCCTGACCACCGAAGCCATCCTGGTGGAAGACG
ACAAGCTCGGCGAAGCCATCGAAAAGCTGTTCGAGAGCGTCACCGGCGGGCTGGACGACCTGGGCGACATCGACCTCGAT
GGCCTGGACATCGAGAATGGCAATACCGAAGCGAAGGAAGATGCGGGCGGGGACGCCGACGATGCGCCCGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGACGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCTTATGAGAAAGTCTACCGAG
TGCGCTTCCGTACCGACGGCATGCTGCACGAGGTCGCCAAGCCGCCAATCCAGCTCGCCACCCGTATCTCCGCGCGCCTG
AAGGTGATGGCTGGCCTGGACATTTCCGAGCGCCGCAAGCCGCAGGACGGCCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCATGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCGCCAGCG
CCCAGTTGGGCATCGATGCGCTGGGTTACGAGGACGCACAGAAGCAGCTCTACCTGGAAGCACTCAAGCAGCCCCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTATCGCTGTATACCGGCATCAATATCCTCAACACCCCGGA
CATCAACATCTCCACCGCCGAAGACCCGGTAGAGATCAACCTCGAAGGCATCAACCAGGTCAACGTCAACCCCAAGCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCTATCAAAGCCGCGCAGACCGGTCACATGGTAATGTCGACGCTGCACACCAACAGCGCCGCGGA
AACCCTCACCCGCCTGCTGAACATGGGCGTGGCCGCCTTCAACCTGGCCACCTCGGTGAACCTGATCATCGCCCAGCGCC
TGGCACGCAAACTTTGCCCTCATTGCAAGAAAGAACACGATGTACCCGCCGAGGCACTGCGTCACGAAGGCTTCCCGGAA
GACAAGATCGGGACTTTCAAATTGTATTCGCCGGTCGGTTGCGATAATTGCAAGGGTGGTTACAAAGGTCGCGTGGGTAT
TTATGAAGTGGTTAAAAACACGCCGGCCCTGCAGCGAATTATCATGGAGGAAGGCAACTCCATTCAGATCGCCGAACAAG
CCCGCAAAGAAGGCTTCAATGATCTGCGCACCTCGGGCCTTCTGAAGGCCATGCAAGGCATCACCAGCCTCGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G6IRV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.829

99.293

0.574
  pilB Acinetobacter baumannii D1279779

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.466

100

0.549
  pilB Vibrio cholerae strain A1552

50.977

99.47

0.507
  pilF Neisseria gonorrhoeae MS11

50.53

100

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

52.126

95.583

0.498
  pilB Vibrio campbellii strain DS40M4

51.386

95.583

0.491
  pilF Thermus thermophilus HB27

38.421

100

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

39.923

92.049

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

93.463

0.367