Detailed information NTG0805268

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P3T65_RS10970
• Genome accession: NZ_CP120376
• Coordinates: 2360006..2360878 (-)
• Length: 290 a.a.
• NCBI ID: WP_038803226.1
• Uniprot ID: -
• Organism: Pseudomonas nitroreducens strain L4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2355006..2365878

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3T65_RS10930 (P3T65_10930)	-	2355143..2356441 (-)	1299	WP_024765641.1	NAD(P)/FAD-dependent oxidoreductase	-
P3T65_RS10935 (P3T65_10935)	-	2356495..2356665 (-)	171	WP_037007365.1	DUF3094 family protein	-
P3T65_RS10940 (P3T65_10940)	-	2356760..2357389 (-)	630	WP_024765640.1	DUF1780 domain-containing protein	-
P3T65_RS10945 (P3T65_10945)	-	2357511..2357945 (+)	435	WP_037007364.1	hypothetical protein	-
P3T65_RS10950 (P3T65_10950)	-	2357942..2358631 (+)	690	WP_024765638.1	energy-coupling factor ABC transporter permease	-
P3T65_RS10955 (P3T65_10955)	-	2358738..2358953 (+)	216	WP_024765637.1	hypothetical protein	-
P3T65_RS10960 (P3T65_10960)	yacG	2359130..2359336 (-)	207	WP_024765636.1	DNA gyrase inhibitor YacG	-
P3T65_RS10965 (P3T65_10965)	coaE	2359333..2359941 (-)	609	WP_024765635.1	dephospho-CoA kinase	-
P3T65_RS10970 (P3T65_10970)	pilD	2360006..2360878 (-)	873	WP_038803226.1	prepilin peptidase	Machinery gene
P3T65_RS10975 (P3T65_10975)	pilC	2360882..2362099 (-)	1218	WP_024765634.1	type II secretion system F family protein	Machinery gene
P3T65_RS10980 (P3T65_10980)	pilB	2362103..2363803 (-)	1701	WP_024765633.1	type IV-A pilus assembly ATPase PilB	Machinery gene
P3T65_RS10985 (P3T65_10985)	-	2364030..2364566 (+)	537	WP_024765632.1	pilin	-
P3T65_RS10990 (P3T65_10990)	tfpZ	2364662..2365384 (+)	723	WP_235376362.1	TfpX/TfpZ family type IV pilin accessory protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32077.09 Da
• Isoelectric Point: 6.9297

>NTDB_id=805268 P3T65_RS10970 WP_038803226.1 2360006..2360878(-) (pilD) [Pseudomonas nitroreducens strain L4]
MSVLDFLASTPLAFVLCATLFGLLVGSFLNVVIHRLPRMMELDWQQQARETLGLPEGEKRPTYNLVLPNSQCPHCSHEIK
PWENIPVVSWLVLGGKCSSCKAPISKRYPLVELACGLLSAFIAWHYGFGWQAVAMLLLTWGLLAMSMIDVDHQLLPDALV
LPLLWLGLIVNYFGLFTSLGDALWGAVFGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGVIMLRLRNAETSTPIPFGPYLAIAGWIAVLWGDQITATYLQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=805268 P3T65_RS10970 WP_038803226.1 2360006..2360878(-) (pilD) [Pseudomonas nitroreducens strain L4]
ATGTCTGTTCTCGACTTCCTGGCCAGCACCCCGCTGGCCTTTGTTTTATGTGCCACCCTCTTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTGGTGATCCATCGCCTGCCGCGGATGATGGAGCTCGACTGGCAGCAGCAGGCGCGGGAAACCCTGGGTC
TGCCGGAGGGCGAGAAGCGACCGACCTATAACCTGGTCCTGCCCAACTCCCAGTGCCCGCACTGCAGCCACGAGATCAAG
CCGTGGGAGAACATCCCGGTGGTCAGTTGGCTGGTGCTAGGCGGCAAGTGCTCGTCGTGCAAGGCACCAATCAGCAAGCG
CTACCCGCTGGTCGAATTGGCCTGTGGCTTGCTCTCGGCCTTTATCGCCTGGCATTACGGCTTCGGCTGGCAGGCCGTGG
CGATGCTGTTGCTGACCTGGGGTTTGTTGGCGATGAGCATGATCGACGTCGATCACCAGTTGCTGCCGGACGCCCTGGTG
CTGCCGCTGCTCTGGCTGGGGCTGATCGTCAACTACTTCGGGCTCTTCACCAGCCTGGGCGACGCGCTCTGGGGTGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGGTCACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGCGCCTGGGGCGGCTGGCAGGTACTGCCGCTGACTATCCTGTTGTCATCGCTGGTAGGC
GCCGTACTCGGCGTGATCATGCTGCGCCTGCGCAACGCCGAGACCAGTACGCCAATCCCCTTCGGCCCCTATCTGGCAAT
CGCCGGCTGGATTGCGGTGCTCTGGGGTGATCAAATAACCGCAACCTATCTGCAGTTCGCCGGTTTCCGCTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	53.333	98.276	0.524
pilD	Acinetobacter baumannii D1279779	51.568	98.966	0.51
pilD	Acinetobacter nosocomialis M2	51.579	98.276	0.507
pilD	Neisseria gonorrhoeae MS11	49.097	95.517	0.469