Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   P3T65_RS03190 Genome accession   NZ_CP120376
Coordinates   685240..687381 (-) Length   713 a.a.
NCBI ID   WP_084358664.1    Uniprot ID   -
Organism   Pseudomonas nitroreducens strain L4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 680240..692381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T65_RS03175 (P3T65_03175) - 681873..683549 (-) 1677 WP_277369026.1 AAA family ATPase -
  P3T65_RS03180 (P3T65_03180) aroB 683556..684662 (-) 1107 WP_024764143.1 3-dehydroquinate synthase -
  P3T65_RS03185 (P3T65_03185) aroK 684713..685204 (-) 492 WP_052239087.1 shikimate kinase AroK -
  P3T65_RS03190 (P3T65_03190) pilQ 685240..687381 (-) 2142 WP_084358664.1 type IV pilus secretin PilQ Machinery gene
  P3T65_RS03195 (P3T65_03195) pilP 687435..687959 (-) 525 WP_024764145.1 type 4a pilus biogenesis lipoprotein PilP -
  P3T65_RS03200 (P3T65_03200) pilO 687956..688609 (-) 654 WP_037010140.1 type 4a pilus biogenesis protein PilO -
  P3T65_RS03205 (P3T65_03205) pilN 688606..689199 (-) 594 WP_024764147.1 type 4a pilus biogenesis protein PilN -
  P3T65_RS03210 (P3T65_03210) comM 689199..690263 (-) 1065 WP_024764148.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 713 a.a.        Molecular weight: 76183.40 Da        Isoelectric Point: 5.4052

>NTDB_id=805253 P3T65_RS03190 WP_084358664.1 685240..687381(-) (pilQ) [Pseudomonas nitroreducens strain L4]
MNRTLSRLSISLLAAVFAPALLAANLQSVDVAALPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLSTLVPYSTRAEGANLYVVVGGAGSASSSYASASPTPSAVAKPVQAKPYIPAGKAIR
NVDFQRGDQGEGNVVIDLSDPTVSPDIQEQGGKIRLDFPRTRLPDNLRVRLDVKDFATPVQFVNASASGDKASILIEPTG
LYDYLVYQTDNRMTVSIKPLTQDDAEKRKKDTFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQISELAPLRRELIQVNYAKASDIAKLFSSVTGAGA
PGAPAAAQGDNRGSITVDDRTNSIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEASVDYSKSLGVRWGGAFTFGNW
ASWGKNGNIGTGGDNGTSCGPFGGGGCTLPTTNNVPTPSVDLGVTNSTSGIGIGFVSNNAILDLQLSAMEKGGNGEVVSQ
PKVVTSDKETAKILKGTEIPYQEASSSGATSVSFKEAALALEVTPQITPDNRIIMEVKVNKDEPDFANALNGIPPIKKNE
VNAKVLVNDGETIVIGGVFSNTQTKAVDKVPFLGDVPVLGRLFRRDSVTDSKNELLVFLTPRIMNNQAIAVGR

Nucleotide


Download         Length: 2142 bp        

>NTDB_id=805253 P3T65_RS03190 WP_084358664.1 685240..687381(-) (pilQ) [Pseudomonas nitroreducens strain L4]
ATGAATAGAACGCTTTCCCGCCTGAGCATTTCCCTGCTCGCGGCCGTATTTGCGCCAGCTTTGCTGGCCGCCAACCTGCA
GAGCGTCGATGTGGCGGCCCTGCCGGGAGACCGCGTGGAGCTCAAACTCGGTTTCGACGAGCCGGTGGCTGCACCGCGTG
GCTATACCATCGAGCAGCCCGCGCGTATCGCACTGGATCTTCCGGGCGTGCAGAACAAGCTGGGTAGCAAGAACCGTGAA
CTGGGTGTGGGGAACGCCCGTAGCCTGACAGTCGTTGAGGCTAAGGATCGAACCCGTCTTATCGTCAACCTGAGCACCCT
GGTGCCTTACAGCACGCGCGCCGAAGGTGCCAACCTGTATGTGGTGGTGGGTGGGGCAGGTTCTGCTTCCAGTTCATACG
CTTCTGCGTCGCCGACTCCCTCGGCAGTTGCCAAGCCTGTGCAGGCCAAGCCTTACATTCCGGCTGGTAAGGCAATCCGT
AATGTGGATTTCCAGCGCGGCGATCAGGGCGAAGGCAACGTCGTAATCGATCTTTCCGATCCGACGGTCAGCCCGGATAT
CCAGGAACAAGGCGGCAAGATCCGTCTGGACTTCCCTCGCACCCGGTTGCCGGACAATCTGCGCGTTCGTCTGGATGTGA
AGGACTTCGCCACGCCGGTGCAGTTCGTCAATGCATCCGCTTCGGGCGACAAGGCCAGCATCTTGATCGAACCGACCGGG
CTGTATGACTATCTGGTCTACCAGACCGACAATCGCATGACCGTCAGCATTAAGCCGCTGACCCAGGACGATGCCGAGAA
GCGCAAGAAAGACACCTTTGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGATGTGCGCTCGGTCTTGC
AGTTGATTGCCGACTTCACCGACCTGAACCTGGTGGCATCGGATACCGTGCAGGGCAACATCACCCTGCGCCTGCAGAAT
GTGCCTTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAATGTGCTGCTGGT
TGCGCCGGCCGATGAAATCGCGGCTCGCGAGCGCCAGGAACTGGAAGCACAGAAGCAGATTTCCGAGCTGGCCCCGCTGC
GCCGTGAGCTGATCCAGGTCAACTACGCCAAGGCATCGGATATCGCCAAACTGTTCAGCTCGGTGACTGGTGCAGGGGCT
CCAGGTGCTCCAGCTGCAGCACAGGGTGATAACCGTGGATCAATCACCGTAGATGATCGTACCAACAGCATCATCGCCTA
CCAGACGCAGGATCGCCTGGATGAGCTGCGCCGTATCGTTGCTCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGG
CGCGTATCGTTGAGGCTTCGGTCGATTACAGCAAGAGTCTCGGGGTCCGTTGGGGGGGTGCCTTTACCTTCGGAAACTGG
GCCAGTTGGGGCAAGAACGGGAACATTGGTACCGGCGGCGATAACGGCACCAGCTGCGGTCCGTTCGGTGGTGGTGGCTG
TACCCTGCCGACAACCAACAACGTTCCTACTCCGTCGGTTGACCTTGGTGTGACCAACAGCACTTCTGGCATCGGCATCG
GCTTCGTGTCCAACAATGCGATTCTGGATCTGCAATTGTCGGCGATGGAGAAGGGCGGTAACGGCGAAGTCGTATCCCAG
CCGAAGGTTGTCACTTCCGACAAGGAAACTGCCAAGATCCTGAAAGGTACGGAGATTCCGTACCAGGAAGCCAGTTCCAG
TGGTGCAACATCGGTTTCCTTCAAGGAAGCTGCATTAGCGCTGGAAGTGACTCCGCAGATCACCCCAGACAATCGCATCA
TCATGGAAGTGAAGGTCAACAAGGACGAGCCGGACTTCGCCAACGCGCTGAACGGCATTCCGCCGATCAAGAAGAACGAA
GTGAACGCCAAGGTGCTGGTCAACGATGGCGAGACCATCGTAATCGGTGGCGTGTTCTCCAACACCCAGACCAAAGCCGT
GGACAAGGTTCCGTTCCTGGGTGACGTTCCGGTGCTGGGCCGACTGTTCCGTCGAGATTCGGTAACCGACAGCAAGAACG
AGCTACTGGTTTTCCTTACCCCGCGGATCATGAATAATCAGGCCATTGCAGTCGGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-449)

Bacterial type II and III secretion system protein (Secretin)

(548-704)

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(303-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

79.694

100

0.804
  comQ Acinetobacter baylyi ADP1

41.313

100

0.424
  pilQ Acinetobacter baumannii strain A118

40.108

100

0.418
  pilQ Acinetobacter baumannii D1279779

40.108

100

0.418
  pilQ Legionella pneumophila strain ERS1305867

38.776

96.213

0.373
  pilQ Legionella pneumophila strain Lp02

38.63

96.213

0.372