Detailed information NTG0805254

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: P3T65_RS03210
• Genome accession: NZ_CP120376
• Coordinates: 689199..690263 (-)
• Length: 354 a.a.
• NCBI ID: WP_024764148.1
• Uniprot ID: A0A1B8VEU1
• Organism: Pseudomonas nitroreducens strain L4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 684199..695263

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3T65_RS03185 (P3T65_03185)	aroK	684713..685204 (-)	492	WP_052239087.1	shikimate kinase AroK	-
P3T65_RS03190 (P3T65_03190)	pilQ	685240..687381 (-)	2142	WP_084358664.1	type IV pilus secretin PilQ	Machinery gene
P3T65_RS03195 (P3T65_03195)	pilP	687435..687959 (-)	525	WP_024764145.1	type 4a pilus biogenesis lipoprotein PilP	-
P3T65_RS03200 (P3T65_03200)	pilO	687956..688609 (-)	654	WP_037010140.1	type 4a pilus biogenesis protein PilO	-
P3T65_RS03205 (P3T65_03205)	pilN	688606..689199 (-)	594	WP_024764147.1	type 4a pilus biogenesis protein PilN	-
P3T65_RS03210 (P3T65_03210)	comM	689199..690263 (-)	1065	WP_024764148.1	pilus assembly protein PilM	Machinery gene
P3T65_RS03215 (P3T65_03215)	-	690449..692878 (+)	2430	WP_024764149.1	penicillin-binding protein 1A	-
P3T65_RS03220 (P3T65_03220)	-	693055..693522 (+)	468	WP_024764150.1	hypothetical protein	-
P3T65_RS03225 (P3T65_03225)	-	693516..694010 (-)	495	WP_084358665.1	M48 family metallopeptidase	-
P3T65_RS03230 (P3T65_03230)	-	694060..694830 (-)	771	WP_024764152.1	class I SAM-dependent methyltransferase	-
P3T65_RS03235 (P3T65_03235)	-	694868..695185 (-)	318	WP_277369029.1	hypothetical protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37761.04 Da
• Isoelectric Point: 4.4486

>NTDB_id=805254 P3T65_RS03210 WP_024764148.1 689199..690263(-) (comM) [Pseudomonas nitroreducens strain L4]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKARTAVKTSAV
AVAGSAVITKTIEMDGGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGTSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFSLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=805254 P3T65_RS03210 WP_024764148.1 689199..690263(-) (comM) [Pseudomonas nitroreducens strain L4]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTGCTGGGGATAGACATCAGTTCGACTTCGGTCAAACTTCTCGAATT
GAGCCGCTCTGGAGGCCGCTACAAGGTAGAGGCCTACGCTGTCGAGCCGCTCCCGCCCAATGCAGTGGTAGAAAAGAACA
TCGCTGAGCTGGAGGGTGTCGGCCAGGCGTTGTCACGTGTGGTCGCCAAGGCGCGGACCGCTGTGAAAACGTCTGCGGTC
GCGGTTGCCGGCTCGGCGGTCATCACCAAAACCATCGAGATGGACGGCGGACTCTCGGACGACGAGCTGGAAAACCAGCT
CAAGATCGAGGCCGACCAGTACATCCCCTATCCCCTCGAAGAGGTTGCCATCGACTTCGAGGTGCAAGGCACATCCGCGC
GCAACGCAGAGCGCGTCGATGTTCTTCTTGCCGCTTGCCGCAAGGAAAACGTCGAGGTGCGCGAAGCTGCGCTGGCGCTC
GCGGGTCTGACAGCCAAGGTCGTCGACGTCGAAGCCTATGCGCTGGAGCGAGCATTCAGTCTGCTGGGAGACCAGCTGGG
CGGCAATGCCGACGAGCTCACAGTTGCGGTGGTCGATATCGGCGCCACCATGACCACGCTGAGCGTCCTGCACCACGGAC
GCACCATCTATACGCGCGAACAGCTCTTCGGTGGGCGTCAGTTGACCGAGGAAATCCAGCGTCGCTATGGCCTGTCGGTC
GAAGAAGCTGGCCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGCTGCAACCGTTCAAGGATGC
CGTGGTCCAGCAGGTGTCTCGTTCCTTGCAGTTCTTTTTCGCCGCCGGTCAGTTCAACGATGTGGATTACATCCTGCTGG
CGGGTGGCACTGCGTCCATCCCTGACCTGGATCGCCTGATCCAGCAGAAGATCGGCACTCCGACCCTGGTGGCCAACCCG
TTCGCCGACATGGTGCTGAGCGGCAAGGTCAACGCCGGTGCGCTGGCCAGCGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1B8VEU1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466