Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PVE35_RS02285 Genome accession   NZ_CP120359
Coordinates   500362..502506 (+) Length   714 a.a.
NCBI ID   WP_277373637.1    Uniprot ID   -
Organism   Pseudomonas sp. AA-38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495362..507506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE35_RS02265 pilM 497527..498591 (+) 1065 WP_268180919.1 pilus assembly protein PilM Machinery gene
  PVE35_RS02270 pilN 498591..499163 (+) 573 WP_268180921.1 type 4a pilus biogenesis protein PilN -
  PVE35_RS02275 pilO 499160..499780 (+) 621 WP_277373635.1 type 4a pilus biogenesis protein PilO -
  PVE35_RS02280 pilP 499780..500304 (+) 525 WP_277373636.1 type 4a pilus biogenesis lipoprotein PilP -
  PVE35_RS02285 pilQ 500362..502506 (+) 2145 WP_277373637.1 type IV pilus secretin PilQ Machinery gene
  PVE35_RS02290 aroK 502512..503030 (+) 519 WP_277373638.1 shikimate kinase AroK -
  PVE35_RS02295 aroB 503082..504185 (+) 1104 WP_268180930.1 3-dehydroquinate synthase -
  PVE35_RS02300 - 504196..505788 (+) 1593 WP_277373639.1 AAA family ATPase -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 76383.76 Da        Isoelectric Point: 5.0024

>NTDB_id=805182 PVE35_RS02285 WP_277373637.1 500362..502506(+) (pilQ) [Pseudomonas sp. AA-38]
MNGSLSCLSAALLMAFLSSSALAANLQGMDVASLPGDRVELKLSFDEPVAVPRGYTIEQPARIALDLPGVANKLGAKNRE
LGAGNARSVTIVEAKDRTRLIVNLGTLAPYSTRVEGNDLYVLVGESTAAPASRPAVAATPVAPAPSKKVYGAPTRAVKNI
DFQRGEQGEGNVVITLSDPSVSPDIQEQGGKIRLDFAKTELPESLRVKLDVKDFATPVQFVSASGAADKTSILIEPVGQY
DYLAYQTDDKLTLSVKPLTQEDAERRSAERFVYKGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVSGNITLRLQNVP
WDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTGTNADA
GGSGAASEERGSLAVDERTNSIIAYQTQERLDELRRIVAQLDIAVRQVMIEARIVEANVDYDKSLGVRWGGTLGGAGSKW
RSYGKDGATGVNPQGAGTCGPFGGNCTRPTVGTTNVPVPFVDMGVVNSSSGIGIGFITDNAILDLQLSAMEKSGNGEIVS
QPKVVTSDKETAKILKGTEVPYQESSSSGATTVAFKEAALALEVTPQITPDNRIIMEVKVNKDEPDYPNAVLGVPPIKKN
EVNAKVLINDGETIVIGGVFSNTRSSAVDKVPFLGDLPFVGRVFRRDVVRDTKSELLVFLTPRIMNNQAIAVNR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=805182 PVE35_RS02285 WP_277373637.1 500362..502506(+) (pilQ) [Pseudomonas sp. AA-38]
ATGAATGGCTCTCTTTCTTGCTTGAGTGCCGCCTTATTGATGGCGTTCTTATCGTCCTCGGCTTTGGCGGCTAATCTTCA
AGGTATGGATGTCGCGAGCTTGCCGGGTGATCGGGTCGAGCTGAAATTGTCCTTCGACGAGCCTGTGGCAGTGCCGCGTG
GCTATACCATCGAGCAGCCAGCGCGTATCGCCCTGGATCTGCCAGGCGTTGCCAACAAGCTCGGCGCGAAGAACCGGGAG
CTTGGCGCTGGCAATGCCCGTAGCGTGACCATAGTTGAGGCCAAGGATCGCACGCGATTGATCGTCAATCTCGGCACCCT
GGCGCCCTACAGCACGCGTGTGGAGGGTAACGATCTCTACGTGCTGGTCGGTGAATCCACTGCTGCGCCGGCCAGCCGAC
CGGCCGTGGCGGCCACCCCGGTCGCTCCAGCTCCGAGCAAGAAGGTCTATGGCGCACCGACCAGGGCCGTGAAGAACATC
GATTTCCAGCGTGGTGAGCAAGGTGAGGGAAATGTCGTCATCACGCTTTCCGATCCCTCCGTCAGCCCTGATATTCAGGA
GCAGGGTGGCAAGATTCGTCTGGACTTCGCCAAGACCGAACTGCCCGAGTCCCTGCGCGTAAAGCTCGATGTGAAGGACT
TCGCTACGCCGGTGCAGTTCGTCAGTGCGAGCGGCGCGGCCGACAAGACCAGCATCCTGATCGAGCCGGTGGGGCAGTAC
GATTATCTCGCCTATCAGACGGACGACAAGCTCACCCTGAGCGTCAAGCCGCTGACCCAGGAAGATGCCGAGCGTCGCAG
TGCCGAGCGCTTCGTCTACAAGGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGCGCTCCGTGCTGCAGTTGA
TCGCGGACTTCACCAACTTGAACCTGGTTGCCAGTGATACCGTTTCCGGCAATATCACTCTGCGTCTGCAGAACGTGCCC
TGGGATCAGGCTCTGGATCTGGTGCTCAAGACCAAGGGACTGGACAAGCGTCAGGTCGGCAACGTGTTGTTGGTTGCACC
GGCGGATGAAATCGCCGCGCGCGAGCGCCAGGAGTTGGAATCGCAGAAGCAGATCGCCGAGCTGGCACCGCTGCGTCGTG
AGTTGATTCAGGTGAACTATGCCAAGGCGGCTGATATAGCCAAGTTGTTCCAGTCGGTTACTGGTACTAATGCGGACGCA
GGGGGTAGTGGGGCTGCGTCTGAAGAGCGTGGTTCGCTGGCTGTCGATGAGCGTACCAATAGTATTATCGCCTACCAGAC
CCAGGAGCGCCTCGATGAGTTGCGCCGTATCGTTGCGCAGTTGGATATCGCAGTGCGTCAGGTCATGATCGAGGCGAGAA
TCGTTGAGGCCAATGTTGATTATGACAAGAGCCTCGGAGTTCGCTGGGGAGGAACCTTGGGCGGTGCAGGTTCCAAGTGG
CGCTCATACGGTAAGGATGGTGCGACTGGTGTTAATCCTCAAGGTGCCGGAACCTGCGGTCCTTTCGGAGGAAACTGTAC
TCGTCCTACAGTGGGTACCACTAACGTGCCTGTACCTTTCGTGGATATGGGGGTGGTGAATAGTTCCTCTGGTATCGGTA
TTGGTTTCATTACCGATAACGCTATTCTGGATTTGCAACTTTCTGCGATGGAGAAGAGTGGTAACGGTGAAATCGTTTCC
CAGCCAAAGGTAGTAACCTCCGATAAGGAAACCGCCAAGATACTCAAGGGTACTGAGGTGCCCTATCAGGAGTCGAGTTC
CAGTGGCGCGACCACTGTCGCCTTCAAGGAAGCTGCTCTTGCTCTGGAGGTCACGCCGCAGATCACTCCTGATAACCGTA
TTATCATGGAAGTTAAAGTGAACAAGGATGAGCCAGACTATCCCAATGCAGTCTTGGGCGTTCCGCCAATCAAGAAGAAT
GAAGTTAACGCGAAGGTGCTTATCAATGATGGGGAAACAATCGTAATCGGTGGTGTGTTCTCCAACACCCGAAGCTCTGC
TGTGGATAAGGTTCCTTTTCTGGGCGATCTGCCTTTCGTGGGGCGTGTCTTCCGCCGCGACGTGGTGCGCGATACCAAAT
CGGAATTGCTTGTCTTCCTTACCCCGCGCATCATGAACAACCAAGCCATTGCCGTGAATCGATGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(549-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-447)

AMIN domain (AMIN)

(39-120)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.997

100

0.749
  comQ Acinetobacter baylyi ADP1

40.47

100

0.41
  pilQ Acinetobacter baumannii strain A118

38.784

100

0.402
  pilQ Acinetobacter baumannii D1279779

38.784

100

0.402
  pilQ Legionella pneumophila strain Lp02

37.341

99.02

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.787

97.479

0.368