Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PVE35_RS02265 Genome accession   NZ_CP120359
Coordinates   497527..498591 (+) Length   354 a.a.
NCBI ID   WP_268180919.1    Uniprot ID   -
Organism   Pseudomonas sp. AA-38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492527..503591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE35_RS02255 - 493487..494755 (+) 1269 WP_268180915.1 malic enzyme-like NAD(P)-binding protein -
  PVE35_RS02260 - 494934..497345 (-) 2412 WP_268180917.1 penicillin-binding protein 1A -
  PVE35_RS02265 pilM 497527..498591 (+) 1065 WP_268180919.1 pilus assembly protein PilM Machinery gene
  PVE35_RS02270 pilN 498591..499163 (+) 573 WP_268180921.1 type 4a pilus biogenesis protein PilN -
  PVE35_RS02275 pilO 499160..499780 (+) 621 WP_277373635.1 type 4a pilus biogenesis protein PilO -
  PVE35_RS02280 pilP 499780..500304 (+) 525 WP_277373636.1 type 4a pilus biogenesis lipoprotein PilP -
  PVE35_RS02285 pilQ 500362..502506 (+) 2145 WP_277373637.1 type IV pilus secretin PilQ Machinery gene
  PVE35_RS02290 aroK 502512..503030 (+) 519 WP_277373638.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37772.12 Da        Isoelectric Point: 4.4647

>NTDB_id=805181 PVE35_RS02265 WP_268180919.1 497527..498591(+) (pilM) [Pseudomonas sp. AA-38]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLSKAKTSVKSAAV
AVAGSAVITKSIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGPAARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYALLESQLGAGHDELTVAIVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDAEVLQPFKEAVVQQVSRSLQFFFAAGQFSDVDYVLLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=805181 PVE35_RS02265 WP_268180919.1 497527..498591(+) (pilM) [Pseudomonas sp. AA-38]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACCTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCTTACGCAGTCGAGCCGCTCCCGCCAAATGCTGTCGTGGAAAAGAACA
TCGCCGAGCTGGAGGGGGTGGGGCAGGCGCTGTCTCGAGTGTTGAGCAAGGCCAAGACGAGCGTCAAATCAGCCGCAGTG
GCCGTTGCCGGCTCCGCCGTGATCACCAAGAGCATCGAGATGGAGGCCGGGCTGTCGGACGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCTTATCCGCTCGAGGAAGTGGCGATCGATTTCGAGGTTCAGGGGCCGGCTGCAC
GTAATCCCGAACGGGTCGAAGTGCTGCTGGCCGCCTGCCGCAAGGAGAACGTCGAAGTGCGTGAGGCCGCCCTCGCCCTG
GCAGGACTGACCGCCAAGGTAGTGGATGTCGAAGCCTATGCCCTCGAGCGTGCTTACGCGCTGCTGGAGAGTCAGTTGGG
CGCCGGTCATGACGAGCTCACCGTGGCCATCGTCGATATCGGTGCCACCATGACTACGCTGAGCGTGCTGCACAACGGTC
GCACCATCTATACCCGCGAGCAGTTGTTCGGTGGCAAGCAGCTCACCGAAGAAATTCAGCGACGCTACGGGCTGTCCGTC
GAGGAAGCCGGGCTGGCCAAGAAGCAGGGTGGCCTGCCGGACGACTACGATGCCGAAGTCCTGCAGCCATTCAAGGAAGC
CGTGGTGCAGCAGGTGTCGCGCTCGCTGCAGTTCTTCTTCGCTGCTGGTCAGTTCAGTGATGTCGATTACGTCCTGCTGG
CAGGCGGAACGGCTTCCATTCCCGACCTTGACCGTCTCATCCAGCAGAAAATCGGTACGCAAACTCTAGTGGCCAACCCT
TTTGCCGATATGGCTCTGGGTAGCAAGGTCAACGCAGGTGCGTTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGCGG
TCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463