Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P3G59_RS24715 Genome accession   NZ_CP119967
Coordinates   5536472..5538172 (+) Length   566 a.a.
NCBI ID   WP_277759332.1    Uniprot ID   -
Organism   Pseudomonas sp. A34-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5531472..5543172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G59_RS24695 - 5531626..5534574 (+) 2949 WP_277759330.1 DUF748 domain-containing protein -
  P3G59_RS24700 - 5534574..5534870 (+) 297 WP_277762201.1 DUF2845 domain-containing protein -
  P3G59_RS24705 - 5535156..5535512 (-) 357 WP_242207033.1 BON domain-containing protein -
  P3G59_RS24710 - 5535809..5536246 (-) 438 WP_277759331.1 pilin -
  P3G59_RS24715 pilB 5536472..5538172 (+) 1701 WP_277759332.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P3G59_RS24720 pilC 5538175..5539392 (+) 1218 WP_277759333.1 type II secretion system F family protein Machinery gene
  P3G59_RS24725 pilD 5539394..5540266 (+) 873 WP_277759334.1 A24 family peptidase Machinery gene
  P3G59_RS24730 coaE 5540263..5540886 (+) 624 WP_277759335.1 dephospho-CoA kinase -
  P3G59_RS24735 yacG 5540883..5541083 (+) 201 WP_007959585.1 DNA gyrase inhibitor YacG -
  P3G59_RS24740 - 5541089..5541304 (-) 216 WP_277759336.1 hypothetical protein -
  P3G59_RS24745 - 5541370..5542059 (-) 690 WP_277759337.1 energy-coupling factor ABC transporter permease -
  P3G59_RS24750 - 5542251..5542877 (+) 627 WP_008078503.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62841.89 Da        Isoelectric Point: 5.9184

>NTDB_id=801253 P3G59_RS24715 WP_277759332.1 5536472..5538172(+) (pilB) [Pseudomonas sp. A34-9]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
LDVESIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=801253 P3G59_RS24715 WP_277759332.1 5536472..5538172(+) (pilB) [Pseudomonas sp. A34-9]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCTGAACTGCTGACAGAAAAAAGTGCCCAGCAAGC
CTGGCAACAGGCCCAGCGTAATCGCCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTGGCAAGTGG
CCGAGATTGCCAGCGAGCATTTCGGCATGGCGTTCCTCGATCTCAATTGCCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGCGAAAAACTTGTGCGTCAGCACCATGCCCTGCCCTTGTGGCGACGTGGCAACAAGCTGTTCGTCGGCATCTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGCACCGGACTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGATGCCATCGAGAAGTTCTTCGACACCCACACCAGTGGCCTGGAAGACATGGCCGATGTCGATCTCGACGGC
CTCGATGTCGAATCCATCGACGACAGCAAGCAGGACGCCATCGGTGGCCTCGATGCCGACGATGCGCCGGTGGTGCGTTT
CGTTCACAAGATGCTGCTCGACGCGATCAAGAGTGGCTCTTCCGACTTGCACTTCGAGCCTTACGAGAAAAACTATCGGG
TGCGGATGCGCACCGACGGCATCCTGCGTGAGGTGGCCAAGCCACCGATTCAACTGGCCGGGCGCATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTCCGCGTCAACACCTTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGTATCGACGCCCTCGGCTATGAGCCCGAGCAGAAGGATCTGTACATGGCCGCGCTCAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGCCCCACCGGGTCGGGTAAAACCGTGTCGCTCTACACCGGCCTGAATATCCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAAG
GACTCGACTTTGCTCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGTGATCTG
GAGACTGCAGAGATTGCGATCAAGGCTGCGCAGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGCCTGCACAATATGGGCATTCCCGGCTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAGAAACCCATCGAAATCCCACGCGAAACCTTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAAGGACGCGTGGGGAT
ATATGAAGTGGTGAAGAACACACCAGAGCTGCAACGTTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGCCGCGACGGCTTTGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.615

99.47

0.583
  pilB Acinetobacter baumannii D1279779

58.333

99.647

0.581
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilB Vibrio campbellii strain DS40M4

48.611

100

0.495
  pilF Thermus thermophilus HB27

36.918

98.587

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362