Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   P3G59_RS02130 Genome accession   NZ_CP119967
Coordinates   481915..483996 (+) Length   693 a.a.
NCBI ID   WP_277760266.1    Uniprot ID   -
Organism   Pseudomonas sp. A34-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476915..488996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G59_RS02110 pilM 479127..480191 (+) 1065 WP_277760262.1 pilus assembly protein PilM Machinery gene
  P3G59_RS02115 - 480191..480757 (+) 567 WP_277760263.1 PilN domain-containing protein -
  P3G59_RS02120 pilO 480754..481377 (+) 624 WP_277760264.1 type 4a pilus biogenesis protein PilO -
  P3G59_RS02125 - 481374..481901 (+) 528 WP_277760265.1 pilus assembly protein PilP -
  P3G59_RS02130 pilQ 481915..483996 (+) 2082 WP_277760266.1 type IV pilus secretin PilQ Machinery gene
  P3G59_RS02135 aroK 484001..484519 (+) 519 WP_277760267.1 shikimate kinase AroK -
  P3G59_RS02140 aroB 484670..485770 (+) 1101 WP_277760268.1 3-dehydroquinate synthase -
  P3G59_RS02145 - 485781..487373 (+) 1593 WP_277760269.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75144.68 Da        Isoelectric Point: 5.5748

>NTDB_id=801240 P3G59_RS02130 WP_277760266.1 481915..483996(+) (pilQ) [Pseudomonas sp. A34-9]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKQLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGAPAPAPRPAAVAPRAAASVAAPAKAFVPKNRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFARTQLPDKLRVRLDVKDFATPVQFVNAAVTADRTVITVEPSG
TYEYSTFQTDNKLTISIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=801240 P3G59_RS02130 WP_277760266.1 481915..483996(+) (pilQ) [Pseudomonas sp. A34-9]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCGCAACCCAAGG
GTTACACCACTGAATCACCGGCGCGGATTGCGCTGGATCTGCCCGGTGTCGCCAGTCAATTGGCCAGCAAGCAACTTGAT
CTGGGTAGCGGTAATGCGCGTACGGCCACCGTGGTCGAAGCCAAGGATCGGACGCGGCTGATCGTCAGCCTCACGCAACT
GGCGCCTTACAGCACGCGGGTCGAGGGTAACAACCTTTTCGTAGTGGTCGGTCAAGGCGCACCGGCACCCGCGCCGCGTC
CTGCCGCCGTGGCACCCCGTGCTGCCGCTAGCGTCGCTGCGCCCGCCAAGGCGTTTGTACCGAAAAACCGCGCGATTCGC
GGTGTGGACTTCCAGCGCGGTACGGCGGGGGAAGGCAATGTCGTCATCGATCTGTCCGATCCGACTATCGCCCCGGATAT
TCAGGAGCATGACGGCAAGATCATCCTCAACTTCGCCCGCACCCAGTTGCCGGACAAGCTGCGGGTGCGCCTCGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGCAGTGACCGCTGATCGCACGGTTATTACCGTCGAGCCCAGCGGC
ACCTATGAGTACTCCACCTTCCAGACCGACAACAAACTGACCATCAGCATCCGTCCGATGACCGTCGATGACCTGCAAAA
ACGTAACGCTGATCGTCAGGCTTACGTCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAACCTGGTCGCCAGCGATACCGTGCAGGGTGGCATCACCTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTTTTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAATGTACTGCTGGT
TGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGGCAGGAACTGGAGTCGCAGAAACAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAGCTGTTGCAAGTGAATTACGCCAAGGCGGCGGACATCGCCAAGCTCTTTCAGTCGGTGACCAGCGCCGAGGCG
AAAATCGACGAACGTGGCTCGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAGCTGCGGCGGATCGTGGCGCAGCTGGATATTCCCGTGCGTCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACG
TCGATTACGACAAAAGCCTGGGCGTACGCTGGGGCGGGTCGATCCAGAACAAGGGCAACTGGAACACTTCGGGCGTCAGC
AATGGCTCATCGACCACCATTGGTACACCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGTACCGTGGGCAA
CACTTCGGGGATCGGTATCGCCTTCATCACTGACAACGTCTTGCTGGATCTCGAGCTGACCGCGATGGAGAAGACCGGCA
ACGGCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCCGACAAGGAAACTGCGAAGATTCTCAAGGGCACCGAGATTCCG
TACCAGGAAGCCAGCTCCAGCGGCGCGACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACCCCGCAGAT
CACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGGGGCGTTTTCTCA
AATACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.045

100

0.755
  comQ Acinetobacter baylyi ADP1

41.21

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.14

100

0.408
  pilQ Acinetobacter baumannii strain A118

38.14

100

0.408
  pilQ Legionella pneumophila strain Lp02

38.018

97.547

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.87

97.547

0.369