Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   P3G59_RS02110 Genome accession   NZ_CP119967
Coordinates   479127..480191 (+) Length   354 a.a.
NCBI ID   WP_277760262.1    Uniprot ID   -
Organism   Pseudomonas sp. A34-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474127..485191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G59_RS02100 - 474876..476144 (+) 1269 WP_038360054.1 malic enzyme-like NAD(P)-binding protein -
  P3G59_RS02105 - 476463..478910 (-) 2448 WP_277760261.1 penicillin-binding protein 1A -
  P3G59_RS02110 pilM 479127..480191 (+) 1065 WP_277760262.1 pilus assembly protein PilM Machinery gene
  P3G59_RS02115 - 480191..480757 (+) 567 WP_277760263.1 PilN domain-containing protein -
  P3G59_RS02120 pilO 480754..481377 (+) 624 WP_277760264.1 type 4a pilus biogenesis protein PilO -
  P3G59_RS02125 - 481374..481901 (+) 528 WP_277760265.1 pilus assembly protein PilP -
  P3G59_RS02130 pilQ 481915..483996 (+) 2082 WP_277760266.1 type IV pilus secretin PilQ Machinery gene
  P3G59_RS02135 aroK 484001..484519 (+) 519 WP_277760267.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38081.64 Da        Isoelectric Point: 4.8109

>NTDB_id=801239 P3G59_RS02110 WP_277760262.1 479127..480191(+) (pilM) [Pseudomonas sp. A34-9]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGASPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=801239 P3G59_RS02110 WP_277760262.1 479127..480191(+) (pilM) [Pseudomonas sp. A34-9]
GTGCTGGGACTCTTCAATAAAAAAACCAATACGTTACTGGGGATCGACATCAGCTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTACGCGGTAGAGCCATTGCCCGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTGGAAGGCGTCGGTCAGGCGCTGAGCCGTGTGCTGGTCAAGGCCCGTACCGGGCTCAAGAGCGTGGCGGTT
GCGGTGGCCGGTTCCGCCGTGATCACCAAAGTCATCGAGATGGACGCCGGTCTTTCCGACGACGAGCTGGAAAACCAGCT
GAAAATCGAAGCCGACCAGTACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGTGCATCGCCAC
GCAACCCCGAGCGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCAGCACTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGCTCGTTCGGCTTGCTCGCGACGCAACTGGC
CGCCTCGCAGGAGCGCCTGACCGTCGCGGTGGTCGACATCGGTGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGGCGTTATGGCCTGACCCTC
GAACAGGCGGGGCTGGCGAAAAAACAGGGTGGCCTGCCCGATGATTACATCAGCGAAGTCCTGCAGCCGTTTCGTGAGGC
GCTGGTACAGCAGGTGTCACGCTCGCTGCAATTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCGTCAGTGCCTGGGCTGGATCGGCTGATCGAGCAGCGCCTGAGCACGCCGACACAAGTGGCCAACCCG
TTTGCCAATATGGCCTTGAGCAGCAAGGTCAACGCCGGCGCGCTGGCAAGCGATGCGCCAGCGCTGATGATCGCCTGCGG
GCTCGCGCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475