Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CupriaWKF_RS14725 Genome accession   NZ_CP119572
Coordinates   3170569..3172299 (+) Length   576 a.a.
NCBI ID   WP_276098584.1    Uniprot ID   -
Organism   Cupriavidus sp. WKF15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3165569..3177299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CupriaWKF_RS14690 (CupriaWKF_14690) proB 3165673..3166791 (-) 1119 WP_276098581.1 glutamate 5-kinase -
  CupriaWKF_RS14695 (CupriaWKF_14695) obgE 3166878..3167975 (-) 1098 WP_276098582.1 GTPase ObgE -
  CupriaWKF_RS14700 (CupriaWKF_14700) rpmA 3168089..3168349 (-) 261 WP_092306246.1 50S ribosomal protein L27 -
  CupriaWKF_RS14705 (CupriaWKF_14705) rplU 3168389..3168700 (-) 312 WP_011299103.1 50S ribosomal protein L21 -
  CupriaWKF_RS14710 (CupriaWKF_14710) - 3168939..3169145 (-) 207 WP_276098583.1 hypothetical protein -
  CupriaWKF_RS14715 (CupriaWKF_14715) ispB 3169195..3170124 (+) 930 WP_276100819.1 octaprenyl diphosphate synthase -
  CupriaWKF_RS14725 (CupriaWKF_14725) pilB 3170569..3172299 (+) 1731 WP_276098584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CupriaWKF_RS14730 (CupriaWKF_14730) pilC 3172331..3173596 (+) 1266 WP_276098585.1 type II secretion system F family protein Machinery gene
  CupriaWKF_RS14735 (CupriaWKF_14735) pilD 3173600..3174484 (+) 885 WP_276098586.1 A24 family peptidase Machinery gene
  CupriaWKF_RS14740 (CupriaWKF_14740) coaE 3174545..3175171 (+) 627 WP_276098587.1 dephospho-CoA kinase -
  CupriaWKF_RS14745 (CupriaWKF_14745) zapD 3175384..3176142 (+) 759 WP_224082031.1 cell division protein ZapD -
  CupriaWKF_RS14750 (CupriaWKF_14750) - 3176147..3176338 (+) 192 WP_011299110.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 63198.75 Da        Isoelectric Point: 7.8002

>NTDB_id=799704 CupriaWKF_RS14725 WP_276098584.1 3170569..3172299(+) (pilB) [Cupriavidus sp. WKF15]
MTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGSGTMSAHDVALFAADKYQLPLLDLTQYNLNKVPPALAGNREF
HAHRLLPLGRRENRLVVAISDPSNQAGLDAIKQKYNLPVDTVVVEHDKLMKHVRSAGEAAGTLKDIAPGATPTQRKLIEY
DPVAAATAQRNRTATSDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIK
VLSRLDISEKRVPQDGRMKLLIPLPQGKDSKEIVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALL
MDTIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDI
IMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTCKRAGSIPHEA
LLDAGFKEAQIDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARRDGVLSLREAGLLKV
KQGVTSLEEVLATTNA

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=799704 CupriaWKF_RS14725 WP_276098584.1 3170569..3172299(+) (pilB) [Cupriavidus sp. WKF15]
ATGACACTTGGTCTCGCCCTTGCACAGAGCCGTCGCATCGCGCCTGCGCTGCTCGCCCAGCTGGAACAGTCCGCGCGCGA
AAAGCAGACGCAGTTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGATGTCGCGCTGTTCGCCGCCGACA
AGTACCAGCTGCCGCTGCTCGACCTGACGCAGTACAACCTGAACAAGGTTCCGCCTGCGCTGGCGGGCAACCGCGAATTT
CATGCTCATCGACTGCTGCCGCTAGGCCGGCGCGAGAACCGCCTGGTGGTGGCGATCTCCGACCCGTCCAACCAGGCCGG
GCTCGACGCCATCAAGCAGAAGTACAACCTGCCGGTCGATACCGTGGTGGTGGAGCACGACAAGCTGATGAAGCATGTGC
GCTCGGCCGGGGAAGCGGCGGGCACGCTTAAGGACATCGCGCCCGGGGCAACGCCCACCCAGCGCAAGCTGATCGAGTAC
GACCCCGTGGCCGCTGCGACTGCGCAGCGCAATCGCACCGCCACCAGCGACATCGACGATGCGCCGGTCGTGCGCTTCCT
GCAGAAGCTGCTGACCGAGGCTTTCCACCGCGGCGCATCAGACCTTCACTTCGAGCCATTCGAAACTTTCTACCGCGTCC
GCTTCCGTGTCGACGGGGTGCTGCAAGAGGTAGCACGCCCGCCGCTCGATATCCGCGACAAGATCGCGACGCGCATCAAG
GTGCTGTCGCGCCTGGACATCTCCGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTGCTGATCCCGCTGCCGCAGGG
CAAGGACTCCAAGGAAATCGTCGAGAAGGCGGTGGACTTCCGCGTGTCGACGCTGCCTACGCTCTTCGGCGAGAAGATCG
TAATGCGTATCCTGGAATCGTCGAGCGACAAGCTGGACATCGACCAGCTCGGCTACGAGCCCGAGCAGAAGGCGCTGCTG
ATGGACACCATCAAGCGCCCATACGGAATGATCCTGGTCACCGGCCCCACCGGCAGCGGCAAGACCGTTTCGCTCTACAC
GTTCCTGAACCTGCTGAACCAGGGCGACATCAATATCTCGACGGCCGAAGACCCGGCTGAAATCCAGCTGCCCGGCATCA
ACCAGGTCAACGTCAACGACAAGGCGGGCCTGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGATATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTACTGTC
GACGCTGCACACCAACGATGCGCCGACTACGCTGACGCGCCTGATGAACATGGGCGTGGCGCCATTCAATATTGCCTCCA
GCGTGCTGCTGATCACGGCGCAGCGGCTGGCGCGGCGGCTGTGCACATGCAAGCGCGCGGGCTCGATCCCGCATGAGGCA
CTGCTCGATGCGGGCTTCAAGGAAGCGCAGATCGATGGCAGCTGGCAGCCTTATCATCCGGTCGGTTGCGAGCGCTGCAA
CGGCAGCGGCTACAAGGGACGCTGCGGCATCTACCAGGTCATGCCGATCACCGAGGCCATGCAGCAGATCATCCTGTCGC
ACGGCACGGCGCTGCAGATTGCCGAACAGGCCCGGCGCGACGGCGTGCTATCGTTGCGGGAAGCCGGCTTGCTGAAGGTC
AAGCAGGGGGTCACGTCACTCGAAGAAGTGCTGGCCACAACCAATGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.826

99.826

0.517
  pilF Neisseria gonorrhoeae MS11

50.775

100

0.512
  pilB Acinetobacter baylyi ADP1

51.971

96.875

0.503
  pilB Legionella pneumophila strain ERS1305867

50

96.528

0.483
  pilB Vibrio cholerae strain A1552

44.367

100

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

45.12

94.271

0.425
  pilB Vibrio campbellii strain DS40M4

45.203

94.097

0.425
  pilF Thermus thermophilus HB27

39.341

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.693

98.264

0.38