Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P0M07_RS20265 Genome accession   NZ_CP119431
Coordinates   4295247..4296953 (+) Length   568 a.a.
NCBI ID   WP_407161101.1    Uniprot ID   -
Organism   Aeromonas caviae strain K523     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4290247..4301953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0M07_RS20240 (P0M07_20280) pdhR 4291425..4292189 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  P0M07_RS20245 (P0M07_20285) ampD 4292466..4293038 (-) 573 WP_151027295.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  P0M07_RS20250 (P0M07_20290) - 4293167..4293637 (+) 471 WP_203763248.1 TIGR02281 family clan AA aspartic protease -
  P0M07_RS20255 (P0M07_20295) nadC 4293641..4294504 (+) 864 WP_102949048.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P0M07_RS20260 (P0M07_20300) - 4294815..4295243 (+) 429 WP_102949047.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P0M07_RS20265 (P0M07_20305) pilB 4295247..4296953 (+) 1707 WP_407161101.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  P0M07_RS20270 (P0M07_20310) pilC 4297081..4298322 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  P0M07_RS20275 (P0M07_20315) pilD 4298400..4299275 (+) 876 WP_201926360.1 A24 family peptidase Machinery gene
  P0M07_RS20280 (P0M07_20320) coaE 4299294..4299908 (+) 615 WP_324038184.1 dephospho-CoA kinase -
  P0M07_RS20285 (P0M07_20325) zapD 4299946..4300668 (+) 723 WP_010675825.1 cell division protein ZapD -
  P0M07_RS20290 (P0M07_20330) yacG 4300679..4300873 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  P0M07_RS20295 (P0M07_20335) mutT 4300929..4301333 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62636.01 Da        Isoelectric Point: 5.8224

>NTDB_id=799292 P0M07_RS20265 WP_407161101.1 4295247..4296953(+) (pilB) [Aeromonas caviae strain K523]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=799292 P0M07_RS20265 WP_407161101.1 4295247..4296953(+) (pilB) [Aeromonas caviae strain K523]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCGCAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAACCCTTTGTCACTTTTCTCATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CAGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCTTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACAGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGATATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAACTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTACAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
TTGGAAACCGCCGAGATTGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCAGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAACCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATTTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.12

0.495
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37