Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P1P91_RS08815 Genome accession   NZ_CP119391
Coordinates   1875222..1876964 (+) Length   580 a.a.
NCBI ID   WP_311882020.1    Uniprot ID   -
Organism   Halomonas piscis strain SG2L-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1866822..1875019 1875222..1876964 flank 203


Gene organization within MGE regions


Location: 1866822..1876964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P1P91_RS08765 (P1P91_08755) - 1867079..1867852 (-) 774 WP_311882012.1 IS110 family transposase -
  P1P91_RS08770 (P1P91_08760) - 1868077..1868715 (-) 639 WP_311882014.1 DUF2335 domain-containing protein -
  P1P91_RS08775 (P1P91_08765) - 1869127..1870506 (-) 1380 WP_311882016.1 sigma-54 dependent transcriptional regulator -
  P1P91_RS08780 (P1P91_08770) - 1871443..1871721 (-) 279 WP_311882017.1 transposase -
  P1P91_RS08785 (P1P91_08775) - 1872038..1872316 (-) 279 WP_311882017.1 transposase -
  P1P91_RS08790 (P1P91_08780) - 1872633..1873667 (-) 1035 WP_311882018.1 IS110 family transposase -
  P1P91_RS08800 (P1P91_08790) - 1874110..1874373 (-) 264 WP_311885754.1 hypothetical protein -
  P1P91_RS08805 (P1P91_08795) pilA 1874370..1874501 (-) 132 WP_311885756.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  P1P91_RS08810 (P1P91_08800) - 1874564..1875019 (-) 456 WP_311882019.1 pilin -
  P1P91_RS08815 (P1P91_08805) pilB 1875222..1876964 (+) 1743 WP_311882020.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63267.42 Da        Isoelectric Point: 5.0857

>NTDB_id=798777 P1P91_RS08815 WP_311882020.1 1875222..1876964(+) (pilB) [Halomonas piscis strain SG2L-4]
MSQPPYESMLSHAAARGGRRGIAERLVRQGLLSDTGLAAAEAAAAREDTELLRYVVEEGLVAPAPAAVAAGREYGLPVLD
LDAVNPEALPPAREYPARILRELQVLPLARRGRLLTVAIPYPATLTQLDELQFATGLRVEGVLAPVDQLAGTLQRYIAQN
ERSMLESLGQIDDAISSLETEDDEYHPELDDDTDDAPIIKFVNKILLDAANSDASDIHFEPYEHLFRVRFRVDGILAEVA
QPPFSLRDRIAARLKVMARLDVSERRLPQDGNIRLRLSRTRSLDFRVNSLPTVYGEKLVLRLLDPAAAQMGIDELGFSEV
QRRQYEKALDKPQGMVLVTGPTGSGKTVTLYTGLNILNQVERNICTAEDPVEIRLPGVNQVNVKPRIGLDFASALRAFLR
QDPDVVMVGEVRDLETAENAVKASQTGHLVLSTLHTNSAAETLVRLSNIGVTAFNIASSVNLIIAQRLVRKLCPACKMPA
EIPPEALAAMGLAEADTAGATLYRAAGCKKCTQGYKGRTGVYEVLPVSETMRGLIMRNASAMEIDALAREEGYVSLYHSG
VQKVLAGITSLEELNRVTKE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=798777 P1P91_RS08815 WP_311882020.1 1875222..1876964(+) (pilB) [Halomonas piscis strain SG2L-4]
ATGAGCCAACCCCCTTACGAGTCCATGCTGAGCCACGCCGCGGCCCGGGGCGGGCGGCGCGGCATCGCCGAACGGCTGGT
GCGCCAGGGACTTTTGAGCGACACCGGCCTCGCCGCCGCCGAAGCCGCGGCCGCCCGGGAGGACACCGAGCTTCTGCGCT
ACGTGGTGGAAGAAGGCCTGGTCGCCCCGGCCCCGGCGGCGGTGGCCGCGGGCCGGGAGTACGGCCTGCCGGTGCTCGAT
CTCGACGCCGTCAACCCCGAGGCGCTGCCCCCGGCCCGGGAATACCCGGCGCGAATCCTGCGCGAGCTGCAGGTGCTGCC
GCTGGCCCGGCGCGGCCGGCTGCTCACCGTGGCCATTCCCTACCCCGCCACGCTCACCCAGCTCGACGAGCTGCAGTTTG
CCACCGGGCTGCGGGTGGAGGGGGTGCTGGCGCCGGTGGACCAGCTCGCCGGCACCCTGCAGCGCTACATCGCCCAGAAC
GAGCGCAGCATGCTGGAAAGCCTGGGCCAGATCGACGACGCCATTTCGTCGCTGGAAACCGAGGACGACGAGTACCACCC
GGAGCTTGACGACGACACCGACGACGCCCCGATCATCAAGTTCGTCAACAAGATACTTCTCGACGCGGCCAACTCCGACG
CCTCGGACATCCACTTCGAGCCCTACGAGCACCTCTTCCGGGTGCGCTTCCGGGTGGACGGCATCCTCGCCGAGGTGGCG
CAGCCGCCGTTTTCCCTGCGCGACCGCATCGCCGCCCGGCTCAAGGTGATGGCGCGGCTGGACGTCTCCGAGCGCCGCCT
GCCCCAGGACGGCAACATCCGCCTGCGGCTGTCGCGCACCCGCTCGCTGGACTTTCGCGTCAACTCGCTGCCCACCGTCT
ACGGCGAGAAGCTGGTGCTGCGCCTGCTCGATCCCGCCGCCGCGCAGATGGGCATCGACGAGCTGGGCTTCAGCGAGGTG
CAGCGCCGCCAGTACGAAAAGGCGCTGGACAAGCCCCAGGGCATGGTGCTGGTGACCGGCCCCACGGGCAGCGGCAAAAC
GGTGACGCTCTACACCGGACTCAACATCCTCAACCAGGTCGAGCGCAACATCTGCACCGCCGAAGACCCGGTGGAAATCC
GCCTGCCCGGGGTCAACCAGGTCAACGTCAAGCCGCGCATCGGGCTCGACTTTGCCAGCGCCCTGCGGGCCTTTCTGCGC
CAGGACCCGGACGTGGTGATGGTGGGCGAAGTCCGCGACCTGGAAACCGCGGAGAACGCCGTCAAGGCCTCGCAGACCGG
CCACCTGGTGCTTTCCACCCTGCACACCAACTCGGCGGCGGAAACCCTGGTACGCCTGTCGAACATCGGCGTGACGGCGT
TCAACATCGCCAGTTCGGTGAACCTGATCATCGCCCAGCGGCTGGTGCGCAAACTCTGCCCGGCCTGTAAAATGCCCGCG
GAGATTCCCCCCGAGGCGCTGGCGGCGATGGGCCTTGCCGAGGCCGACACCGCCGGGGCCACGCTCTACCGCGCGGCGGG
CTGCAAGAAGTGCACCCAGGGCTACAAGGGCCGAACCGGCGTCTACGAGGTACTTCCGGTCAGCGAAACCATGCGCGGGC
TGATCATGCGCAACGCCAGCGCCATGGAGATCGACGCCCTGGCTCGGGAGGAAGGCTACGTCAGCCTCTACCACAGCGGC
GTGCAGAAGGTGCTGGCCGGTATCACCAGCCTCGAGGAACTCAACCGGGTCACCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-161)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

49.474

98.276

0.486
  pilB Legionella pneumophila strain ERS1305867

50.089

97.069

0.486
  pilB Acinetobacter baylyi ADP1

47.295

98.793

0.467
  pilB Vibrio cholerae strain A1552

51.473

87.759

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

53.388

83.966

0.448
  pilF Neisseria gonorrhoeae MS11

47.339

93.966

0.445
  pilB Vibrio campbellii strain DS40M4

53.249

82.241

0.438
  pilB/pilB1 Synechocystis sp. PCC 6803

39.964

94.483

0.378
  pilF Thermus thermophilus HB27

38.214

96.552

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.504

94.483

0.364