Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PY247_RS03415 Genome accession   NZ_CP119223
Coordinates   743132..744844 (+) Length   570 a.a.
NCBI ID   WP_159725455.1    Uniprot ID   A0A653K627
Organism   Acinetobacter proteolyticus strain AP54     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 738132..749844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY247_RS03380 (PY247_03380) nusA 738951..740437 (-) 1487 Protein_659 transcription termination factor NusA -
  PY247_RS03385 (PY247_03385) rimP 740475..740999 (-) 525 WP_004656212.1 ribosome maturation factor RimP -
  PY247_RS03405 (PY247_03405) secG 741626..741955 (-) 330 WP_004656213.1 preprotein translocase subunit SecG -
  PY247_RS03410 (PY247_03410) tpiA 741968..742759 (-) 792 WP_159725454.1 triose-phosphate isomerase -
  PY247_RS03415 (PY247_03415) pilB 743132..744844 (+) 1713 WP_159725455.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PY247_RS03420 (PY247_03420) pilC 744869..746095 (+) 1227 WP_004656216.1 type II secretion system F family protein Machinery gene
  PY247_RS03425 (PY247_03425) - 746109..746962 (+) 854 Protein_665 A24 family peptidase -
  PY247_RS03430 (PY247_03430) coaE 746964..747569 (+) 606 WP_004656219.1 dephospho-CoA kinase -
  PY247_RS03435 (PY247_03435) - 747566..748477 (-) 912 WP_101235316.1 DMT family transporter -
  PY247_RS03440 (PY247_03440) rlmB 748507..749253 (-) 747 WP_065343633.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PY247_RS03445 (PY247_03445) - 749388..749714 (-) 327 WP_004656225.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63283.56 Da        Isoelectric Point: 5.0134

>NTDB_id=798206 PY247_RS03415 WP_159725455.1 743132..744844(+) (pilB) [Acinetobacter proteolyticus strain AP54]
MSGLHTSPKFSGFIRRLVEDGHMSTTIMQTAIDTAKKSQQDIVAHLIEQHRLSPLTIAETIAAEFGEPLFDLAVYDTALL
PRDLADNKLIQKHKVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGESED
FDLDIDVSQDPSKEEDDAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCGQCKEEINIPKQSLLELGF
TEQDLNDPEFKIFQPVGCAECREGYKGRVGIYEVMKVTPEISRLIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=798206 PY247_RS03415 WP_159725455.1 743132..744844(+) (pilB) [Acinetobacter proteolyticus strain AP54]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTAGTTGAAGATGGACACATGTCCACGACAAT
CATGCAGACCGCAATTGATACGGCTAAAAAATCACAACAAGATATCGTTGCACATTTGATTGAACAGCATCGCTTATCAC
CGCTGACCATTGCGGAAACTATTGCTGCTGAATTTGGTGAACCACTTTTCGATCTGGCCGTATACGATACAGCCTTACTG
CCTCGAGATCTGGCGGATAACAAACTCATTCAAAAGCATAAAGTTGCTCCGCTGCTACAACGTGGTCAAATTTTATATGT
GGCGACCAGCAACCCCACCAATATCGAAGCGTTGGATGCGATTCGCTTCAATACCAAACTGAATATTGAACCGATTATTG
TTGAGCACACTAAACTTGAAAAACTGATTGAACAGCAGTTTGGGGACTCTGGCAGTTTTGACTTTGGTGAAAGTGAAGAC
TTTGATCTCGACATTGATGTAAGTCAAGACCCAAGCAAAGAAGAGGACGACGCGCCACAAGGTGATGAGTCTCCGATTGT
CAAATATATTAATAAACTGTTGATTGATGCGATTCGTATGGGTGCATCTGACCTGCATTTTGAACCCTATGAAAAAATGT
ATCGAGTACGTTACCGTGTCGATGGTGTATTGCGTCAGATTGCCAATCCACCCTTACAAATGGCAACCCGTCTTGCCTCC
CGTTTAAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGGATGCCACAAGATGGACGTATTAAACTCAAATTATCAAA
AACCAAGGCGATTGATTTCCGTGTCAACTCTCTGCCAACATTATTTGGTGAAAAGTTAGTTCTGCGTATTCTCGATCCGT
CCAGCGCCATGTTAGGGATTGAAGCACTCGGTTATGAGGATGAGCAAAAAGCCTTATTTATGGATGCACTGGATAAACCA
CAAGGTATGCTGCTCATCACTGGCCCAACAGGTTCAGGTAAAACCGTGTCTTTGTATACGGGTTTAAACATCCTCAATAC
TGAAGATACCAACATTTCGACCGCCGAAGACCCTGTCGAAATTAACCTGGAAGGCATCAATCAGGTTAACGTCAATCCGA
AAACAGGACTTACCTTCTCTGCAGCGCTGAAAGCCTTCTTACGTCAAGATCCTGATGTGATCATGGTCGGGGAGATTCGT
GATCTGGAAACGGCTGAAATTGCCATTAAAGCCGCACAAACGGGTCATATGGTGATGTCAACCCTGCATACCAACAGCGC
ACCAGAAACCTTGACGCGTCTACGCAACATGGGAGTGCCTTCTTTCAACATTGCAACCTCCGTCAATCTGGTGATTGCAC
AACGCTTGGCGCGTCGCCTCTGTGGTCAATGTAAAGAAGAGATTAATATCCCCAAACAAAGCTTGTTGGAACTTGGCTTT
ACTGAACAAGATTTAAATGACCCTGAATTCAAAATTTTTCAACCTGTAGGTTGTGCTGAATGCCGTGAAGGCTATAAAGG
TCGTGTGGGGATTTACGAAGTCATGAAGGTCACGCCAGAAATTTCCAGACTAATTATGGAAGATGGCAATGCCTTACAGA
TTGCAGCCGCATCAGAACAAGCAGGATTTAATAATCTACGTCGTTCAGGTCTGAAAAAAGTCATGCAAGGAATTACCTCT
TTGCAAGAAGTCAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A653K627

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.137

100

0.823
  pilB Acinetobacter baylyi ADP1

76.265

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.846

93.509

0.504
  pilF Neisseria gonorrhoeae MS11

49.027

99.123

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

48.741

97.544

0.475
  pilB Vibrio cholerae strain A1552

49.094

96.842

0.475
  pilB Vibrio campbellii strain DS40M4

51.252

91.053

0.467
  pilF Thermus thermophilus HB27

37.857

98.246

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

33.876

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.722

93.333

0.361