Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PY824_RS03375 Genome accession   NZ_CP119172
Coordinates   603706..604371 (+) Length   221 a.a.
NCBI ID   WP_039670586.1    Uniprot ID   A0A380K4J8
Organism   Streptococcus macedonicus strain SGM CIP105683     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598706..609371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY824_RS03355 (PY824_03355) - 600126..601706 (+) 1581 WP_014294203.1 DEAD/DEAH box helicase -
  PY824_RS03360 (PY824_03360) - 601749..602021 (-) 273 WP_014294204.1 GIY-YIG nuclease family protein -
  PY824_RS03365 (PY824_03365) - 601996..602757 (-) 762 WP_115266022.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PY824_RS03370 (PY824_03370) - 602865..603611 (+) 747 WP_014294206.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  PY824_RS03375 (PY824_03375) comEA 603706..604371 (+) 666 WP_039670586.1 helix-hairpin-helix domain-containing protein Machinery gene
  PY824_RS03380 (PY824_03380) comEC/celB 604364..606595 (+) 2232 WP_115266023.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PY824_RS03385 (PY824_03385) holA 606667..607704 (+) 1038 WP_039670612.1 DNA polymerase III subunit delta -
  PY824_RS03390 (PY824_03390) sodA 607793..608401 (+) 609 WP_014294210.1 superoxide dismutase SodA -
  PY824_RS03395 (PY824_03395) - 608552..609277 (+) 726 WP_014294211.1 YebC/PmpR family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 23662.45 Da        Isoelectric Point: 4.3842

>NTDB_id=798043 PY824_RS03375 WP_039670586.1 603706..604371(+) (comEA) [Streptococcus macedonicus strain SGM CIP105683]
MIEEIKEKLLENKTLVAMLGTILVMLIGFFAWSNVTKTTVDAQNDLPALSTTFSASSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSNGRRVTDVVKLAGGFTEDADKKSVNLAEKVTDESMIYVARVGENVAPATTNSQVNGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide


Download         Length: 666 bp        

>NTDB_id=798043 PY824_RS03375 WP_039670586.1 603706..604371(+) (comEA) [Streptococcus macedonicus strain SGM CIP105683]
ATGATTGAAGAAATAAAAGAAAAACTTTTAGAGAACAAAACGTTGGTAGCGATGTTGGGAACAATACTTGTCATGTTAAT
CGGATTTTTTGCTTGGTCAAACGTGACAAAAACAACAGTGGATGCACAAAATGACTTACCTGCCCTAAGCACGACTTTTT
CGGCAAGTAGCAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAAACTCAAAAAGTGTTTGTAGATATC
AAAGGTGCTGTGAAAAATGAGGGTGTTTATGAGCTTTCAAACGGTAGGCGTGTGACAGATGTGGTTAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATTTGGCTGAAAAAGTAACCGATGAATCAATGATTTATGTGGCAAGAG
TTGGTGAAAATGTTGCTCCTGCAACGACTAATTCACAAGTAAACGGTAGCGCTCAGCAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCAGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGACATTATTGACTA
TCGTGATGCCAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGCATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(78-131)

Helix-hairpin-helix motif (HHH_3)

(157-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380K4J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Streptococcus thermophilus LMD-9

47.639

100

0.502
  comEA/celA/cilE Streptococcus mitis SK321

47.059

100

0.471
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

45.909

99.548

0.457
  comEA/celA/cilE Streptococcus mitis NCTC 12261

45.455

99.548

0.452
  comEA/celA/cilE Streptococcus pneumoniae Rx1

44.545

99.548

0.443
  comEA/celA/cilE Streptococcus pneumoniae D39

44.545

99.548

0.443
  comEA/celA/cilE Streptococcus pneumoniae R6

44.545

99.548

0.443
  comEA Latilactobacillus sakei subsp. sakei 23K

38.813

99.095

0.385