Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PX653_RS15615 Genome accession   NZ_CP119083
Coordinates   3552501..3554189 (+) Length   562 a.a.
NCBI ID   WP_277413692.1    Uniprot ID   -
Organism   Pseudoduganella chitinolytica strain ACM 3522     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3547501..3559189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX653_RS15595 (PX653_15595) - 3547575..3549098 (-) 1524 WP_277413688.1 carboxylesterase/lipase family protein -
  PX653_RS15600 (PX653_15600) uraH 3549189..3549542 (+) 354 WP_277413689.1 hydroxyisourate hydrolase -
  PX653_RS15605 (PX653_15605) - 3549527..3550753 (-) 1227 WP_277413690.1 glutamate carboxypeptidase -
  PX653_RS15610 (PX653_15610) - 3551087..3552358 (+) 1272 WP_277413691.1 HlyC/CorC family transporter -
  PX653_RS15615 (PX653_15615) pilF 3552501..3554189 (+) 1689 WP_277413692.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PX653_RS15620 (PX653_15620) pilC 3554200..3555426 (+) 1227 WP_277413693.1 type II secretion system F family protein Machinery gene
  PX653_RS15625 (PX653_15625) pilD 3555571..3556449 (+) 879 WP_277413694.1 A24 family peptidase Machinery gene
  PX653_RS15630 (PX653_15630) coaE 3556446..3557068 (+) 623 Protein_3087 dephospho-CoA kinase -
  PX653_RS15635 (PX653_15635) zapD 3557270..3558025 (+) 756 WP_277413695.1 cell division protein ZapD -
  PX653_RS15640 (PX653_15640) yacG 3558038..3558217 (+) 180 WP_277413696.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 60967.64 Da        Isoelectric Point: 6.3228

>NTDB_id=797586 PX653_RS15615 WP_277413692.1 3552501..3554189(+) (pilF) [Pseudoduganella chitinolytica strain ACM 3522]
MSGLARALMQAGRLTPPQADALQKKSHADKLPFIDVLLASGIVNARDLALFCAETFAYPMLDLQAFAPAALPVKLIDQKL
MQSQRVVALAKRGNKMSVAISDPTNTQALDQIKFQTESSVEPVIVPHDALVRLLQELGKSSEQLMGELAGDEGEIQFAEE
QESTSVAEAPATDVEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLIEHAQPPIAIKDKLVSRIKVLAR
LDISEKRVPQDGRMRLIVSPTKTIDLRISTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKALLLDAIARPYGMVLVT
GPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIMVGEIRDLETADI
AIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSIVLITAQRLARRLCTCKQPVDISNDLLRRAGFKAHDLDGS
WKPYGPVGCERCNGTGYKGRVGIYQIMPITPAIEALILAHGNAMQIAAQSESEGVKSLRQSGLVKVKAGLTSLEEVLGCT
NE

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=797586 PX653_RS15615 WP_277413692.1 3552501..3554189(+) (pilF) [Pseudoduganella chitinolytica strain ACM 3522]
ATGTCGGGCCTGGCACGTGCCTTGATGCAGGCAGGGCGGCTCACGCCCCCGCAGGCGGACGCGCTGCAAAAGAAATCCCA
CGCCGACAAGCTCCCGTTCATCGACGTGCTGCTGGCCAGCGGCATCGTCAACGCGCGCGACCTGGCGCTGTTCTGTGCCG
AGACGTTCGCTTATCCGATGCTGGACCTGCAGGCGTTCGCACCGGCCGCGCTGCCCGTCAAGCTGATCGACCAGAAGCTG
ATGCAGAGCCAGCGCGTGGTGGCGCTGGCCAAGCGCGGCAACAAGATGTCGGTGGCGATCTCCGACCCCACCAACACGCA
GGCGCTGGACCAGATCAAGTTCCAGACCGAGTCGTCGGTGGAACCCGTCATCGTGCCGCACGACGCACTGGTGCGCCTGC
TGCAGGAACTGGGCAAGAGCAGCGAACAGCTGATGGGCGAGCTGGCCGGCGACGAAGGCGAGATCCAGTTCGCCGAGGAA
CAGGAATCGACGTCCGTGGCGGAAGCGCCGGCGACCGACGTCGAGGACGCGCCCATCGTGCGCTTCCTGAACAAGATGCT
GATGGACGCCGTCAACATGGGCGCCTCCGACCTGCACTTCGAACCGTTCGAGAAGTTCTACCGCATCCGCTTCCGTGTCG
ACGGCGTACTGATCGAGCATGCGCAGCCGCCCATCGCCATCAAGGACAAGCTGGTCTCGCGCATCAAGGTGCTGGCGCGC
CTGGACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGCATGCGCCTGATCGTCTCGCCCACGAAGACCATCGACCTGCG
CATCTCGACCCTGCCGACGCTGTTCGGCGAGAAGACCGTGATGCGGATCCTGGACGCGACGCAGGCCCAGATGGGCATCG
ACGCGCTGGGCTACGATCCGGACCAGAAGGCGCTGCTGCTGGACGCCATCGCCCGGCCCTATGGCATGGTGCTGGTGACG
GGGCCGACGGGCTCCGGCAAGACCGTGTCGCTGTACACGTGCCTGAACATCCTCAACAAGCCCGGCATCAATATCTCGAC
GGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCGTGAACCAGGTCAACGTCAACGACAAGGCGGGGCTGACCTTCCCCG
TCGCGCTGAAGTCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGCGAGATCCGCGACCTGGAGACGGCCGACATC
GCCATCAAGGCGGCGCAGACGGGCCACATGGTGTTCTCTACCTTGCACACGAACGACGCGCCGTCCACCCTCACGCGCCT
GATGAACATGGGCGTGGCGCCGTTCAATATCGCATCGTCGATCGTGTTGATCACGGCGCAGCGCCTGGCGCGCCGCCTGT
GCACGTGCAAGCAGCCGGTGGACATCTCGAACGACCTGCTGCGGCGGGCCGGCTTCAAGGCGCATGACCTGGACGGCAGC
TGGAAGCCGTACGGCCCGGTGGGCTGCGAGCGCTGCAACGGCACCGGCTACAAGGGCCGGGTGGGCATCTACCAGATCAT
GCCGATCACGCCGGCCATCGAGGCGCTGATCCTGGCGCACGGCAATGCGATGCAGATCGCCGCGCAGTCGGAAAGCGAGG
GCGTGAAGTCGCTGCGCCAGTCCGGCCTCGTGAAGGTCAAGGCGGGCCTCACCAGCCTGGAAGAAGTGCTGGGCTGTACC
AACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(185-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.464

100

0.536
  pilB Acinetobacter baylyi ADP1

52.837

100

0.53
  pilB Acinetobacter baumannii D1279779

55.871

93.95

0.525
  pilB Legionella pneumophila strain ERS1305867

48.754

100

0.488
  pilB Vibrio cholerae strain A1552

46.277

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

44.602

100

0.448
  pilB Vibrio campbellii strain DS40M4

44.523

100

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.286

99.644

0.391
  pilF Thermus thermophilus HB27

38.251

97.687

0.374