Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PYU99_RS18745 Genome accession   NZ_CP118993
Coordinates   4086638..4088344 (+) Length   568 a.a.
NCBI ID   WP_042649025.1    Uniprot ID   -
Organism   Aeromonas media strain K521     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4081638..4093344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU99_RS18720 (PYU99_18720) pdhR 4082761..4083525 (-) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PYU99_RS18725 (PYU99_18725) ampD 4083802..4084374 (-) 573 WP_182787467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PYU99_RS18730 (PYU99_18730) - 4084520..4084981 (+) 462 WP_371316622.1 retropepsin-like aspartic protease family protein -
  PYU99_RS18735 (PYU99_18735) nadC 4084985..4085848 (+) 864 WP_270824598.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PYU99_RS21780 - 4086173..4086634 (+) 462 WP_082030008.1 pilin -
  PYU99_RS18745 (PYU99_18745) pilB 4086638..4088344 (+) 1707 WP_042649025.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PYU99_RS18750 (PYU99_18750) pilC 4088474..4089715 (+) 1242 WP_042649026.1 type II secretion system F family protein Machinery gene
  PYU99_RS18755 (PYU99_18755) pilD 4089796..4090668 (+) 873 WP_042649027.1 prepilin peptidase Machinery gene
  PYU99_RS18760 (PYU99_18760) coaE 4090687..4091301 (+) 615 WP_042649028.1 dephospho-CoA kinase -
  PYU99_RS18765 (PYU99_18765) zapD 4091339..4092061 (+) 723 WP_042649029.1 cell division protein ZapD -
  PYU99_RS18770 (PYU99_18770) yacG 4092071..4092265 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  PYU99_RS18775 (PYU99_18775) mutT 4092341..4092745 (-) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62696.11 Da        Isoelectric Point: 5.8224

>NTDB_id=797208 PYU99_RS18745 WP_042649025.1 4086638..4088344(+) (pilB) [Aeromonas media strain K521]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDNVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=797208 PYU99_RS18745 WP_042649025.1 4086638..4088344(+) (pilB) [Aeromonas media strain K521]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCCTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTAG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGACTTTGGCTTCAGCTTCGGTCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCTGATGAGAGTTCGCGGCTCGACGACAACGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTAGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCAGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCACGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.473

99.648

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37