Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PYW33_RS01375 Genome accession   NZ_CP118963
Coordinates   325307..327016 (-) Length   569 a.a.
NCBI ID   WP_004644929.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain DSM 2403     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320307..332016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW33_RS01345 - 320426..320749 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  PYW33_RS01350 rlmB 320884..321633 (+) 750 WP_004281091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PYW33_RS01355 - 321640..322563 (+) 924 WP_004644926.1 DMT family transporter -
  PYW33_RS01360 coaE 322585..323184 (-) 600 WP_004281093.1 dephospho-CoA kinase -
  PYW33_RS01365 pilD 323186..324046 (-) 861 WP_004281094.1 prepilin peptidase Machinery gene
  PYW33_RS01370 pilC 324046..325275 (-) 1230 WP_004281096.1 type II secretion system F family protein Machinery gene
  PYW33_RS01375 pilB 325307..327016 (-) 1710 WP_004644929.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PYW33_RS01380 tpiA 327294..328085 (+) 792 WP_004281100.1 triose-phosphate isomerase -
  PYW33_RS01385 secG 328099..328428 (+) 330 WP_004281101.1 preprotein translocase subunit SecG -
  PYW33_RS01415 rimP 329387..329911 (+) 525 WP_004281103.1 ribosome maturation factor RimP -
  PYW33_RS01420 nusA 329946..331430 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63586.74 Da        Isoelectric Point: 4.7884

>NTDB_id=796907 PYW33_RS01375 WP_004644929.1 325307..327016(-) (pilB) [Acinetobacter lwoffii strain DSM 2403]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLFPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFRNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKDLFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=796907 PYW33_RS01375 WP_004644929.1 325307..327016(-) (pilB) [Acinetobacter lwoffii strain DSM 2403]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCATTAGCGCATGCCAAACAAGAAAAAATCGATATTGTGGCTGAGTTAATTAACCAGCAGCAACTTTTTC
CAACCGTCATTGCTGAAACGATTTCAGTCGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCTCAAATT
CTTCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATTCTGCCGATTTTTAGAAATTCCAGTATTTTATATGT
TGCGACCAGTAACCCGACCAATATCGAAGCGATTGATGCAGTCCGCTTTTCCACCAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGTACCTTTGAATTCGATGAAGACTTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCACCGATTGTCAA
ATATATTAATAAATTACTAATTGATGCGATCCGTATGGGGGCTTCAGACTTACATTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTAGATGGGGTATTACGCCAGATTGCAACACCACCACTACAGCTGGCAAATCGCTTGTCTTCGCGT
CTTAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGTGAGAAGCTGGTACTGCGAATTCTGGATCCCTCCA
GCGCGATGTTGGGAATTGATGCACTAGGTTATGAACCGGAACAAAAAGACCTTTTTATGCAAGCGCTGGACAAACCGCAA
GGCATGCTCCTGATCACTGGTCCAACAGGTTCCGGTAAAACCGTTTCACTGTATACCGGTCTGAATATCCTCAATCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTACAAGGCATTAATCAAGTCAACGTCAACAATAAAG
TTGGTCTTACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGACATCGTAATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCGGCGCAGACCGGTCATATGGTGATGTCGACCTTGCATACCAATAGTGCGCC
TGAGACCTTGACCCGTTTACGAAACATGGGGGTGCCCTCTTTCAATATTGCCACTTCAGTAAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGTTTGTGTTCACAATGCAAGAAACCTGCAGATATTCCGCAACAAAGCTTACTGGAGATGGGCTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGTCGTGAAGGCTATAAGGGTCG
TGTAGGTATTTATGAAGTTATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CAGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.175

100

0.803
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

50.883

99.473

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

49.374

98.243

0.485
  pilB Vibrio campbellii strain DS40M4

48.746

98.067

0.478
  pilF Neisseria gonorrhoeae MS11

48.485

98.594

0.478
  pilB Vibrio cholerae strain A1552

50.48

91.564

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

35.073

100

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.763

100

0.371
  pilF Thermus thermophilus HB27

37.168

99.297

0.369