Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PYW33_RS01365 Genome accession   NZ_CP118963
Coordinates   323186..324046 (-) Length   286 a.a.
NCBI ID   WP_004281094.1    Uniprot ID   N9QGW2
Organism   Acinetobacter lwoffii strain DSM 2403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318186..329046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW33_RS01340 recN 318322..319983 (-) 1662 WP_004644925.1 DNA repair protein RecN -
  PYW33_RS01345 - 320426..320749 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  PYW33_RS01350 rlmB 320884..321633 (+) 750 WP_004281091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PYW33_RS01355 - 321640..322563 (+) 924 WP_004644926.1 DMT family transporter -
  PYW33_RS01360 coaE 322585..323184 (-) 600 WP_004281093.1 dephospho-CoA kinase -
  PYW33_RS01365 pilD 323186..324046 (-) 861 WP_004281094.1 prepilin peptidase Machinery gene
  PYW33_RS01370 pilC 324046..325275 (-) 1230 WP_004281096.1 type II secretion system F family protein Machinery gene
  PYW33_RS01375 pilB 325307..327016 (-) 1710 WP_004644929.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PYW33_RS01380 tpiA 327294..328085 (+) 792 WP_004281100.1 triose-phosphate isomerase -
  PYW33_RS01385 secG 328099..328428 (+) 330 WP_004281101.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32010.35 Da        Isoelectric Point: 8.0624

>NTDB_id=796905 PYW33_RS01365 WP_004281094.1 323186..324046(-) (pilD) [Acinetobacter lwoffii strain DSM 2403]
MHDFLQYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQMFLHPEQPVIDETKLSLSKPASACPKCHQP
IRWYQNIPVISWLVLRGKCSNCQNPISIRYPLVEILTAVCALLVVAVFGPTLQMLFGLILTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTLPGAAIWGYIIGFLCLWVVYYAFKIVTGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=796905 PYW33_RS01365 WP_004281094.1 323186..324046(-) (pilD) [Acinetobacter lwoffii strain DSM 2403]
ATGCACGATTTTCTTCAATATTTTATTCAAAACCCAACGGCGTTGTATATCGCAGTTGGGCTTTTTAGTTTATGTATCGG
CAGCTTTCTGAATGTCGTGATTTACCGCACTCCCAAGATGATGGAACAGGAATGGCGTACTGATTGCCAGATGTTCCTGC
ATCCGGAACAGCCGGTGATTGATGAAACTAAGTTAAGTTTAAGCAAACCCGCTTCAGCTTGCCCCAAGTGTCATCAGCCA
ATCCGCTGGTACCAGAATATTCCCGTCATTAGCTGGCTGGTCCTGCGCGGAAAATGTAGCAACTGCCAGAATCCGATCAG
CATCCGTTATCCTTTGGTTGAAATTCTGACGGCAGTTTGTGCACTCCTTGTCGTAGCAGTATTTGGCCCTACCCTACAAA
TGCTGTTTGGCCTGATCCTGACTTATGTGCTGATCGCACTCACTTTTATTGATTTTGACACCCAGTTATTGCCTGACCGT
TATACTTTACCCTTGGCCGCGCTAGGTTTGGGGGTAAATAGTTATGCACTTTATACCTTACCCGGTGCTGCCATCTGGGG
CTATATTATTGGTTTCCTGTGTCTGTGGGTGGTGTATTATGCATTTAAAATCGTTACCGGCAAGGAAGGTATGGGCTATG
GCGATTTTAAATTGCTTGCCGCCCTGGGTGCCTGGATGGGGCCTTTATTACTGCCTTTAATTGTGCTGCTGTCTTCTCTG
GTTGGTGCGATTATCGGGATTATCCTACTGAAAGTCCGCAAGGAAAATCAGCCTTTTGCCTTTGGTCCCTATATCGCGAT
TGCCGGCTGGATTGCTTTTTTGTGGGGCGAGCAGATTATGAAAGTGTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QGW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Acinetobacter baumannii D1279779

79.649

99.65

0.794
  pilD Vibrio cholerae strain A1552

51.237

98.951

0.507
  pilD Vibrio campbellii strain DS40M4

49.826

100

0.5
  pilD Neisseria gonorrhoeae MS11

46.909

96.154

0.451