Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PX335_RS01710 Genome accession   NZ_CP118933
Coordinates   350604..352316 (-) Length   570 a.a.
NCBI ID   WP_017385371.1    Uniprot ID   A0A429KCK1
Organism   Acinetobacter pittii strain ML4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 345604..357316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX335_RS01680 (PX335_01680) - 345768..346094 (+) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -
  PX335_RS01685 (PX335_01685) rlmB 346197..346946 (+) 750 WP_016142380.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  PX335_RS01690 (PX335_01690) - 346982..347896 (+) 915 WP_017385368.1 DMT family transporter -
  PX335_RS01695 (PX335_01695) coaE 347893..348489 (-) 597 WP_017385369.1 dephospho-CoA kinase -
  PX335_RS01700 (PX335_01700) pilD 348491..349351 (-) 861 WP_017385370.1 A24 family peptidase Machinery gene
  PX335_RS01705 (PX335_01705) pilC 349351..350577 (-) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  PX335_RS01710 (PX335_01710) pilB 350604..352316 (-) 1713 WP_017385371.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PX335_RS01715 (PX335_01715) tpiA 352608..353402 (+) 795 WP_017385372.1 triose-phosphate isomerase -
  PX335_RS01720 (PX335_01720) secG 353415..353744 (+) 330 WP_016142373.1 preprotein translocase subunit SecG -
  PX335_RS01740 (PX335_01740) rimP 354382..354906 (+) 525 WP_002114922.1 ribosome maturation factor RimP -
  PX335_RS01745 (PX335_01745) nusA 354944..356428 (+) 1485 WP_002115134.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63579.96 Da        Isoelectric Point: 5.0932

>NTDB_id=796492 PX335_RS01710 WP_017385371.1 350604..352316(-) (pilB) [Acinetobacter pittii strain ML4]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESNFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=796492 PX335_RS01710 WP_017385371.1 350604..352316(-) (pilB) [Acinetobacter pittii strain ML4]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAACTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTAGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTTCGTTATCGAGTCGATGGAGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAATCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTCAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A429KCK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.912

100

0.949
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.323

99.474

0.511
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381
  pilF Thermus thermophilus HB27

36.833

98.596

0.363