Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ORD17_RS04020 Genome accession   NZ_CP118747
Coordinates   770272..771972 (+) Length   566 a.a.
NCBI ID   WP_308389600.1    Uniprot ID   -
Organism   Acidithiobacillus sp. AMEEHan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765272..776972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORD17_RS04005 - 767805..768290 (+) 486 WP_308389597.1 peptidylprolyl isomerase -
  ORD17_RS04010 - 768312..769448 (+) 1137 WP_308389598.1 anhydro-N-acetylmuramic acid kinase -
  ORD17_RS04015 - 769442..770296 (+) 855 WP_308389599.1 shikimate dehydrogenase -
  ORD17_RS04020 pilB 770272..771972 (+) 1701 WP_308389600.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ORD17_RS04025 - 772010..773563 (+) 1554 WP_308389601.1 ATP-binding protein -
  ORD17_RS04030 pilR 773560..774699 (+) 1140 WP_308389602.1 sigma-54 dependent transcriptional regulator Regulator
  ORD17_RS04035 - 774741..774923 (+) 183 WP_308389603.1 hypothetical protein -
  ORD17_RS04040 - 774907..775710 (+) 804 WP_308389604.1 prepilin peptidase -
  ORD17_RS04045 coaE 775707..776303 (+) 597 WP_308389605.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62224.88 Da        Isoelectric Point: 5.1250

>NTDB_id=794960 ORD17_RS04020 WP_308389600.1 770272..771972(+) (pilB) [Acidithiobacillus sp. AMEEHan]
MAANLEAITLTPLLRGLVGAGLADENSLRALLNDPTRGKQPLLPFLVEKKAISAIALVEYLSERYKMPMLDLDAVQLDET
VIRRIDRALLERHQVLPISLHRDALYLAMADPTDVKATEDIRFNAGGLQINPILVEANKLAKAVAEFSGGFKNQLEEFFA
PDDAQQEQDEFDLAGDARGEDAPVVRFVQQLLLDAIQKGVSDIHIEPYERDIRVRYRLDGVLQDMLHPPIGLRDGITSRL
KILCRLDISERRLPQDGRLRVRIPPARIIDFRVSFLPTNFGETIVLRLLDPASSKVPIEALGFLPQQLRAFDDAIHRPYG
MILVTGPTGSGKTTTLYTALNILNTGDVNISTAEDPVEIPVYGINQVNINERIGLNFAAALRSFLRQDPDVIMVGEVRDL
ETAETAVKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGSVHLVMAQRLVRKLCVACKKPEKIPEAALLEAGFAP
EDLPGLQPMGPVGCEQCNGGYRGRMGLYQVMPISEAMREVILQGGSAMDLSRQAAAEGILSMRQSGLLRVKEGITSLEEV
LRVTNL

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=794960 ORD17_RS04020 WP_308389600.1 770272..771972(+) (pilB) [Acidithiobacillus sp. AMEEHan]
ATGGCTGCAAACCTCGAAGCTATAACCTTGACCCCCCTTTTGCGTGGGCTCGTCGGGGCAGGCTTGGCAGATGAGAACTC
TCTTCGCGCCCTGCTGAACGATCCCACACGCGGCAAGCAGCCGCTGCTTCCTTTTCTGGTAGAGAAGAAAGCGATTTCGG
CAATCGCCTTGGTGGAATATCTCTCCGAACGCTACAAAATGCCGATGCTCGACCTGGATGCCGTGCAACTGGACGAGACC
GTCATTCGTCGCATCGATCGAGCGCTTCTGGAGCGGCATCAGGTCCTCCCCATTTCGTTGCATCGCGACGCGCTCTATCT
GGCCATGGCCGACCCCACCGATGTCAAGGCTACCGAAGACATCCGCTTCAATGCTGGCGGTCTGCAGATCAATCCCATTC
TGGTCGAGGCCAACAAGCTGGCCAAGGCGGTTGCGGAATTCTCGGGCGGGTTCAAGAATCAGCTCGAAGAATTTTTCGCC
CCCGATGACGCCCAGCAAGAACAGGATGAATTTGATCTGGCCGGTGATGCCCGCGGGGAGGACGCCCCCGTCGTCCGCTT
TGTCCAGCAGCTCCTCCTCGATGCGATTCAAAAGGGCGTATCGGACATTCATATCGAGCCCTACGAACGGGACATTCGCG
TGCGCTATCGCCTTGATGGCGTGCTGCAGGACATGCTCCACCCCCCGATCGGTCTGCGCGATGGCATCACATCTCGGCTG
AAGATCCTCTGTCGTCTCGATATTTCCGAACGGCGCCTGCCCCAGGATGGACGCCTGCGGGTTCGCATCCCCCCGGCACG
AATCATCGATTTCCGCGTCTCTTTCTTGCCCACCAACTTCGGCGAGACCATTGTGCTGCGTCTGCTCGATCCGGCCAGCT
CGAAGGTGCCCATCGAAGCGCTGGGCTTCCTGCCACAGCAATTGCGCGCCTTTGACGATGCCATCCACCGGCCCTACGGG
ATGATCCTCGTTACCGGTCCGACCGGATCCGGCAAAACCACGACCCTGTATACCGCGCTCAACATCCTCAACACCGGCGA
CGTCAATATCAGCACGGCGGAGGATCCGGTAGAAATCCCCGTTTATGGCATCAATCAGGTGAACATCAACGAGCGCATCG
GGCTCAACTTTGCCGCGGCCCTCCGCTCCTTTCTGCGTCAGGATCCCGATGTGATCATGGTCGGCGAGGTGCGCGATCTG
GAAACCGCCGAGACCGCCGTGAAGGCGGCCCAGACCGGACACTTGGTGCTCGCCACCCTGCACACCAATGACGCGCCGCA
AAGCCTGACCCGCTTGGAAAATATGGGAATTCCGACCTACAACATCGCCGGCAGCGTGCATCTGGTCATGGCACAGCGCC
TAGTACGAAAGCTTTGTGTCGCCTGCAAAAAGCCCGAAAAAATCCCGGAGGCGGCGCTCTTGGAAGCAGGCTTCGCGCCC
GAGGATCTCCCAGGTCTGCAGCCCATGGGACCGGTCGGCTGCGAACAGTGCAACGGCGGCTATCGCGGCCGTATGGGTCT
TTATCAGGTCATGCCCATCAGCGAGGCCATGCGCGAGGTCATTCTGCAGGGGGGGTCGGCCATGGATCTGTCCCGTCAGG
CCGCGGCGGAAGGCATTCTCAGCATGCGTCAGTCCGGTCTCTTGCGGGTAAAGGAAGGCATCACCAGTCTTGAAGAAGTC
CTTCGCGTCACCAACCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.835

95.23

0.484
  pilB Acinetobacter baumannii D1279779

46.087

100

0.468
  pilB Acinetobacter baylyi ADP1

47.491

98.587

0.468
  pilB Vibrio cholerae strain A1552

47.619

92.756

0.442
  pilF Neisseria gonorrhoeae MS11

44.704

98.41

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.731

93.11

0.426
  pilB Vibrio campbellii strain DS40M4

46.408

90.989

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

35.669

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.535

98.763

0.39
  pilF Thermus thermophilus HB27

38.889

98.587

0.383