Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PYG29_RS01665 Genome accession   NZ_CP118686
Coordinates   373170..374849 (-) Length   559 a.a.
NCBI ID   WP_239423046.1    Uniprot ID   -
Organism   Snodgrassella communis strain B10998     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368170..379849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYG29_RS01635 (PYG29_01640) - 368499..369416 (-) 918 WP_239423041.1 ABC transporter ATP-binding protein -
  PYG29_RS01640 (PYG29_01645) yacG 369465..369662 (-) 198 WP_239423042.1 DNA gyrase inhibitor YacG -
  PYG29_RS01645 (PYG29_01650) zapD 369662..370423 (-) 762 WP_239423043.1 cell division protein ZapD -
  PYG29_RS01650 (PYG29_01655) coaE 370420..371034 (-) 615 WP_100101728.1 dephospho-CoA kinase -
  PYG29_RS01655 (PYG29_01660) pilD 371031..371882 (-) 852 WP_239423045.1 A24 family peptidase Machinery gene
  PYG29_RS01660 (PYG29_01665) pilG 371889..373121 (-) 1233 WP_100121065.1 type II secretion system F family protein Machinery gene
  PYG29_RS01665 (PYG29_01670) pilF 373170..374849 (-) 1680 WP_239423046.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PYG29_RS01670 (PYG29_01675) recJ 375056..376753 (-) 1698 WP_309618753.1 single-stranded-DNA-specific exonuclease RecJ -
  PYG29_RS01675 (PYG29_01680) - 376750..377721 (-) 972 WP_239423047.1 hypothetical protein -
  PYG29_RS01680 (PYG29_01685) - 377769..379535 (-) 1767 WP_309619064.1 PDZ domain-containing protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61937.36 Da        Isoelectric Point: 5.7285

>NTDB_id=794351 PYG29_RS01665 WP_239423046.1 373170..374849(-) (pilF) [Snodgrassella communis strain B10998]
MSVGLIRVLYKHQIIDAEQAEKLRQELEQKRPVISALLQSGTINADKLASFLSGVFSIPYLNLELYSQNLVPLDLINLKI
MQQHMCVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQLLENLDHESSNLLDGMDSIGDTEATGSN
AMSADEEDGENGPIAKFVFKILGDALAGGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRSQVASRIKVMSKMDISE
KRIPQDGRIQVQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCSCKAPAERPPEYALKKAGFSDEDLAKDWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSEEMQRIIMNNGNEVDISTQALKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=794351 PYG29_RS01665 WP_239423046.1 373170..374849(-) (pilF) [Snodgrassella communis strain B10998]
ATGAGTGTAGGATTGATTCGGGTTTTGTATAAGCACCAGATTATTGACGCAGAACAAGCAGAAAAACTGCGTCAGGAGCT
GGAGCAGAAACGTCCGGTTATATCGGCACTGTTGCAATCTGGTACCATTAATGCCGATAAGCTGGCCAGCTTCCTGTCTG
GAGTATTTAGTATTCCTTACCTGAATCTGGAACTGTATAGCCAAAATCTGGTACCGTTAGATTTAATCAATCTGAAAATT
ATGCAGCAACACATGTGCGTACCGATATTTAAGCGCGGTAACCGCCTCTTTCTTGCTGTATCTGACCCTACATCAATTCA
ACATTACCAGAAAATTGCTACCGATGCTGGCTGTATACTGAACTTAGTTATTGTTCGTGATGATCAGTTGCGTCAGTTAC
TGGAAAATCTTGATCATGAATCCAGCAACCTGCTGGACGGCATGGATTCAATTGGTGACACAGAAGCAACTGGCAGCAAC
GCCATGTCTGCTGATGAGGAAGATGGCGAAAATGGGCCAATTGCCAAATTTGTCTTCAAAATACTGGGGGATGCTCTGGC
TGGCGGGGCATCGGATATCCATTTCGAATTTTATGAAGAAATGGCACGGGTGCGTTTCCGTATTGATGGACAATTACGTG
AAGTAGTGCGTATGCCTCTGAATATTCGCAGTCAGGTTGCCTCTCGCATTAAAGTCATGTCCAAAATGGATATTTCGGAA
AAACGTATTCCTCAGGATGGTCGTATTCAAGTTCAGTTCCGCAAAGGTGCCAAACCAATTGATTTTCGTGTCAGCACACT
GCCCACGCTGTTTGGTGAAAAAATTGTAATGCGTATTCTGAATTCGGATGCAGCCACCCTCAATATCGAACAGCTCGGTT
TTGAACCATTCCAGAAAGAGTTACTGCTGGAAGCCATTCACCGCCCCTATGGCATGGTGCTAGTTACCGGTCCAACCGGT
TCCGGTAAAACTGTATCACTTTATACCTGTCTGAATATACTGAATACCGACAGCGTTAATATTTCTACGGCCGAAGACCC
TGCGGAAATTAACTTGCCTGGTATCAATCAGGTCAACGTCAATGATAAACAGGGGCTGACATTTGCTGCAGCCCTGAAAT
CTTTCCTCCGTCAGGATCCGGATATTATCATGGTGGGGGAGATTCGAGATCTGGAAACCGCAGATATCGCCATCAAGGCT
GCACAAACAGGCCATATGGTTTTCTCCACCCTACATACCAATAACGCTCCGGCCACATTATCGCGTATGCTGAATATGGG
CGTTGCACCATTCAACATTGCCAGCTCTGTTAATCTGATTATGGCTCAGCGTCTGGTACGACGTTTATGTAGCTGCAAGG
CACCTGCAGAACGTCCACCTGAATATGCTTTGAAAAAAGCCGGCTTCTCGGATGAAGATCTGGCTAAAGACTGGACATTT
TACCGCCCAGTAGGCTGTGAACGTTGCAAAGGTAAGGGCTACAAGGGACGTGCTGGTGTTTATGAAGTCATGCCTGTCAG
CGAAGAAATGCAACGTATCATTATGAACAACGGTAATGAAGTTGATATCAGCACGCAGGCGCTCAAAGAAGGACTGGTTG
ATTTACGTCATGCCGGTTTGCTTAAAGTTATGGAAGGCATCACGTCTCTGGACGAAATTCTGGCAACCACCAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.214

100

0.683
  pilB Acinetobacter baylyi ADP1

50.796

100

0.513
  pilB Acinetobacter baumannii D1279779

53.084

95.707

0.508
  pilB Legionella pneumophila strain ERS1305867

48.506

100

0.494
  pilB Vibrio campbellii strain DS40M4

44.19

100

0.449
  pilB Vibrio cholerae strain A1552

44.17

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

46.053

95.17

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.789

95.17

0.388
  pilF Thermus thermophilus HB27

39.179

95.886

0.376