Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PWW25_RS11320 Genome accession   NZ_CP118584
Coordinates   2441341..2441769 (+) Length   142 a.a.
NCBI ID   WP_009698754.1    Uniprot ID   A0A8B3DN92
Organism   Vibrio harveyi strain fish5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2436341..2446769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW25_RS11305 pdhR 2438373..2439140 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PWW25_RS11310 ampD 2439545..2440096 (-) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW25_RS11315 nadC 2440189..2441076 (+) 888 WP_029790346.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW25_RS11320 pilA 2441341..2441769 (+) 429 WP_009698754.1 pilin Machinery gene
  PWW25_RS11325 pilB 2441769..2443454 (+) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW25_RS11330 pilC 2443488..2444711 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW25_RS11335 pilD 2444784..2445653 (+) 870 WP_274876439.1 A24 family peptidase Machinery gene
  PWW25_RS11340 coaE 2445654..2446268 (+) 615 WP_274876440.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14524.57 Da        Isoelectric Point: 5.7554

>NTDB_id=793654 PWW25_RS11320 WP_009698754.1 2441341..2441769(+) (pilA) [Vibrio harveyi strain fish5]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSEAAAGLATLRSLTTNIDTFIADTGTFPADADAPTLGAA
VDMNKLGTISFADSGASGATATFTFDGTASALASTDTVVLAKDATTGLWTCSHSTGVTLKGC

Nucleotide


Download         Length: 429 bp        

>NTDB_id=793654 PWW25_RS11320 WP_009698754.1 2441341..2441769(+) (pilA) [Vibrio harveyi strain fish5]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATCGTGGTTGCGGTTATTGGTGTTTT
GGCAGCGATTGCTATTCCACAGTATCAGAATTATGTAAAAAAATCAGAGGCTGCAGCTGGGCTTGCAACTCTTCGTTCAC
TAACTACAAATATTGATACCTTCATTGCTGATACTGGCACCTTTCCTGCTGATGCCGACGCACCCACGTTAGGTGCTGCT
GTTGATATGAACAAACTTGGAACAATTTCTTTTGCAGATAGTGGTGCATCGGGAGCGACAGCAACGTTTACTTTTGATGG
TACAGCTAGCGCACTAGCTTCTACTGATACCGTTGTCCTTGCGAAGGATGCAACTACCGGACTTTGGACCTGCTCTCATA
GTACTGGCGTAACTCTAAAAGGCTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B3DN92

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.299

100

0.556
  pilA Vibrio cholerae strain A1552

51.299

100

0.556
  pilA Vibrio cholerae C6706

51.299

100

0.556
  pilA Vibrio parahaemolyticus RIMD 2210633

48.951

100

0.493
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472
  pilA Acinetobacter baumannii strain A118

35.294

100

0.38