Detailed information NTG0079305

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HRS36_RS11280
• Genome accession: NZ_AP022839
• Coordinates: 2377702..2378760 (-)
• Length: 352 a.a.
• NCBI ID: WP_173237388.1
• Uniprot ID: A0A6F8T6Y1
• Organism: Legionella antarctica strain TUM19329
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2372702..2383760

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HRS36_RS11255 (TUM19329_22720)	aroK	2373089..2373616 (-)	528	WP_018576163.1	shikimate kinase AroK	-
HRS36_RS11260 (TUM19329_22730)	pilQ	2373887..2376007 (-)	2121	WP_173237384.1	type IV pilus secretin PilQ	Machinery gene
HRS36_RS11265 (TUM19329_22750)	pilP	2376017..2376541 (-)	525	WP_173237385.1	pilus assembly protein PilP	Machinery gene
HRS36_RS11270 (TUM19329_22760)	pilO	2376538..2377137 (-)	600	WP_173237386.1	type 4a pilus biogenesis protein PilO	Machinery gene
HRS36_RS11275 (TUM19329_22770)	pilN	2377152..2377697 (-)	546	WP_173237387.1	PilN domain-containing protein	Machinery gene
HRS36_RS11280 (TUM19329_22780)	pilM	2377702..2378760 (-)	1059	WP_173237388.1	type IV pilus assembly protein PilM	Machinery gene
HRS36_RS11285 (TUM19329_22790)	-	2379086..2380159 (+)	1074	WP_173237389.1	hypothetical protein	-
HRS36_RS11290 (TUM19329_22800)	-	2380308..2382680 (+)	2373	WP_173237390.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38425.06 Da
• Isoelectric Point: 4.5289

>NTDB_id=79305 HRS36_RS11280 WP_173237388.1 2377702..2378760(-) (pilM) [Legionella antarctica strain TUM19329]
MLKLFQSRQHSILGVDISSSSVKILEISSSGDGVCVESYGRELLPLNALDGNVIKDIEAVSNCIKKLIVNARLTCKKVAL
AVPDSAVISKVVQINDGLNNEEMEELIAIEADKYIPYPIDEINLDFEILGHSAKNSAMLDVLIVASRAENVNSRVEAVNR
AGLTATIVDVESYAVERAAQQLAMPAGGQDKIVAIIDIGARFSHLFVLHGMKLVFSREEKFGSIQLVDAIAEHYQMTQQQ
VIEAQAQRTLPDDYDSEVLEPFKETLLLQVKRTLQFFYSTSQDGFVDHILLAGGLARQPDLATLIQEQLGVSTTVANPLS
HMSFSEKIDIAAVTQDAPSLMVACGLALRNME

Nucleotide

• Length: 1059 bp

>NTDB_id=79305 HRS36_RS11280 WP_173237388.1 2377702..2378760(-) (pilM) [Legionella antarctica strain TUM19329]
GTGCTCAAACTCTTTCAATCCAGGCAGCACTCCATCTTGGGAGTAGACATAAGCTCATCATCAGTGAAAATATTAGAAAT
TTCCAGCTCTGGAGATGGGGTTTGTGTTGAAAGTTATGGGCGTGAATTATTACCCTTAAATGCTCTTGACGGAAATGTGA
TTAAGGATATTGAAGCGGTATCAAACTGTATTAAAAAACTTATTGTTAATGCACGTTTGACCTGTAAAAAAGTGGCATTA
GCTGTTCCTGATTCTGCAGTTATCAGTAAAGTGGTACAAATCAACGATGGTTTAAATAATGAGGAAATGGAAGAGCTGAT
TGCTATAGAGGCTGATAAGTACATCCCATATCCGATAGATGAAATTAATCTTGACTTTGAAATTCTAGGTCATTCTGCAA
AAAACTCAGCAATGTTAGACGTACTAATAGTTGCCTCAAGGGCCGAAAATGTAAACAGTCGTGTGGAAGCAGTAAATCGT
GCGGGGTTAACCGCAACAATTGTTGATGTGGAGTCTTATGCAGTTGAGCGTGCAGCGCAACAGCTGGCAATGCCAGCTGG
GGGGCAAGATAAAATAGTAGCAATTATTGATATTGGGGCAAGGTTCAGTCATTTGTTCGTATTGCATGGAATGAAGTTAG
TCTTCTCAAGAGAGGAGAAGTTTGGGAGTATCCAACTTGTTGATGCTATTGCTGAACATTACCAAATGACACAACAGCAA
GTCATCGAGGCTCAAGCTCAAAGAACATTACCTGATGATTATGATTCTGAGGTATTAGAGCCTTTTAAAGAAACGCTTTT
ATTACAGGTAAAACGTACTTTACAGTTTTTCTATTCTACCAGTCAGGATGGTTTTGTGGATCATATTTTACTCGCTGGCG
GGTTGGCCAGGCAGCCAGATCTTGCTACGTTAATTCAAGAGCAGCTCGGTGTGAGTACTACAGTTGCAAACCCTTTATCT
CACATGTCTTTTAGTGAAAAAATTGATATTGCGGCTGTAACCCAAGATGCTCCTTCTTTAATGGTGGCCTGTGGCTTGGC
TCTAAGAAATATGGAGTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-350)

3D structure

• AlphaFold DB: A0A6F8T6Y1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	71.751	100	0.722
comM	Acinetobacter nosocomialis M2	42.655	100	0.429
comM	Acinetobacter baylyi ADP1	42.135	100	0.426
pilM	Acinetobacter baumannii D1279779	42.373	100	0.426