Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PU634_RS01545 Genome accession   NZ_CP118224
Coordinates   323778..325490 (-) Length   570 a.a.
NCBI ID   WP_306762327.1    Uniprot ID   A0AA50KPW8
Organism   Oceanimonas pelagia strain NTOU-MSR1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318778..330490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PU634_RS01525 (PU634_01525) trmB 319928..320644 (+) 717 WP_306762323.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  PU634_RS01530 (PU634_01530) coaE 320719..321345 (-) 627 WP_306762324.1 dephospho-CoA kinase -
  PU634_RS01535 (PU634_01535) pilD 321342..322196 (-) 855 WP_306762325.1 A24 family peptidase Machinery gene
  PU634_RS01540 (PU634_01540) pilC 322526..323752 (-) 1227 WP_306762326.1 type II secretion system F family protein Machinery gene
  PU634_RS01545 (PU634_01545) pilB 323778..325490 (-) 1713 WP_306762327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PU634_RS17215 - 325497..325940 (-) 444 WP_371319616.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PU634_RS01555 (PU634_01555) nadC 326234..327088 (-) 855 WP_306762328.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PU634_RS01560 (PU634_01560) - 327138..327656 (-) 519 WP_306762329.1 TIGR02281 family clan AA aspartic protease -
  PU634_RS01565 (PU634_01565) ampD 327729..328271 (+) 543 WP_306762330.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PU634_RS01570 (PU634_01570) pdhR 328745..329506 (+) 762 WP_306762331.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62639.16 Da        Isoelectric Point: 5.6993

>NTDB_id=791101 PU634_RS01545 WP_306762327.1 323778..325490(-) (pilB) [Oceanimonas pelagia strain NTOU-MSR1]
MTIPVNTSRSGLARSLAASSLLSATELDQVQTLARQEGKPLSTMLVEKQLLSSRELARFCEREYGQPLLDLDDFDIEQLP
PQFLSMDLIERHHAIPVKLDNRVLYLAMSDPTHVAALEDFGFRFNLVTDTLLVEEDKLQQLLLRLQQSGGTGLDIDGIGD
DDIAGLELGEDPGERDDEVGRNEDDAPIVRYINKIMLDAVRKEASDLHFEPFERFFRIRFRIDGMLHEVASPPVHLAGRF
AARLKVMARLDIAERRLPQDGRIKLKLTASKSVDMRVNTLPTMWGEKVVIRILDSSAARLDIDQLGYSEAQKALYLKALK
RPQGMILVTGPTGSGKTVSLYTGLSILNTVTANISTAEDPVEINLPGINQVQINPKAGLSFAHALRAFLRQDPDIIMVGE
IRDLETAEISVKAAQTGHLVLSTLHTNSAAETLTRLGNMGLPAYNIASSVSLIMAQRLARKLCPHCRAPEVLPTDQLTRL
GFTPAQLAEGITLYRAVGCPQCTEGYKGRIGVYEVMAMSERLAHLIMEGAGSLALARAAEEEGMVTLRRAALDKARLGII
SLAEVNRITG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=791101 PU634_RS01545 WP_306762327.1 323778..325490(-) (pilB) [Oceanimonas pelagia strain NTOU-MSR1]
ATGACCATCCCCGTTAATACCTCCAGAAGCGGCCTGGCCCGAAGCCTGGCCGCTTCTTCGCTGCTGTCTGCCACCGAGCT
GGATCAGGTGCAGACCCTGGCCCGGCAGGAAGGCAAGCCCCTCAGCACCATGCTGGTGGAAAAACAACTGCTGTCTTCCC
GGGAGCTGGCCCGCTTCTGCGAGCGGGAATACGGCCAGCCATTGCTGGATCTCGACGACTTCGACATCGAACAGTTGCCG
CCCCAGTTTCTGAGCATGGATCTGATCGAGCGCCACCACGCCATTCCGGTCAAGCTGGACAACCGGGTGCTCTACCTGGC
CATGTCGGATCCCACCCATGTGGCGGCGCTGGAAGACTTCGGCTTTCGCTTCAACCTGGTCACCGACACCCTGCTGGTGG
AGGAAGACAAGCTGCAGCAATTGCTGCTGCGCCTGCAGCAAAGCGGCGGCACCGGGTTGGATATTGACGGCATCGGCGAC
GACGACATCGCCGGTCTGGAGCTGGGGGAAGACCCCGGCGAGCGCGACGACGAGGTGGGCCGCAATGAAGACGACGCCCC
CATCGTGCGCTACATCAACAAGATCATGCTGGATGCGGTACGCAAGGAAGCCTCGGATCTGCACTTTGAGCCCTTTGAGC
GCTTTTTCCGCATTCGCTTTCGCATCGACGGCATGCTGCACGAGGTGGCCTCGCCACCGGTGCACCTGGCCGGCCGTTTT
GCCGCCCGGCTCAAGGTGATGGCCCGGCTCGACATTGCCGAGCGCCGCCTGCCCCAGGACGGCCGCATCAAGCTCAAGCT
GACCGCGTCCAAGTCGGTGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGGTCGTTATTCGTATTCTCG
ACAGCTCCGCCGCCCGCCTCGATATCGATCAGCTGGGCTACAGCGAGGCACAGAAGGCGCTGTACCTCAAGGCGCTGAAA
CGCCCCCAGGGCATGATACTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGGCCTGAGCATTCT
CAATACCGTGACCGCCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGTATCAACCAGGTGCAGATCA
ACCCCAAGGCCGGGCTGTCCTTTGCCCATGCCCTGCGCGCCTTTCTGCGCCAGGATCCCGACATCATCATGGTGGGGGAA
ATCCGCGATCTGGAAACCGCCGAGATCTCGGTCAAGGCGGCGCAAACCGGCCACCTGGTGCTGTCTACCCTGCACACCAA
CTCCGCCGCCGAAACCCTCACCCGGCTCGGCAACATGGGACTGCCGGCCTACAACATCGCCTCATCGGTCAGCCTGATCA
TGGCCCAGCGGCTGGCGCGCAAACTGTGCCCCCACTGCCGTGCCCCCGAGGTGCTGCCCACCGACCAGCTGACCCGGCTG
GGGTTTACCCCCGCCCAGCTGGCCGAGGGCATTACCCTGTACCGGGCGGTGGGCTGCCCCCAGTGCACCGAGGGCTACAA
GGGCCGCATCGGCGTGTATGAGGTTATGGCCATGAGCGAGCGTCTGGCCCACCTGATCATGGAAGGCGCCGGCTCTCTGG
CCCTGGCCCGGGCGGCGGAAGAGGAAGGCATGGTCACCCTGCGCCGGGCGGCCCTGGACAAGGCGCGGCTCGGCATTATC
AGCCTGGCGGAAGTCAACCGCATCACCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.369

98.947

0.528
  pilB Vibrio cholerae strain A1552

52.566

99.123

0.521
  pilB Acinetobacter baylyi ADP1

50.977

98.772

0.504
  pilB Acinetobacter baumannii D1279779

50.89

98.596

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.265

99.474

0.5
  pilB Vibrio campbellii strain DS40M4

50.178

98.596

0.495
  pilF Neisseria gonorrhoeae MS11

48.208

97.895

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

37.641

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.194

95.789

0.375