Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PotMrB4_RS26120 Genome accession   NZ_AP022642
Coordinates   5644363..5646489 (-) Length   708 a.a.
NCBI ID   WP_172434850.1    Uniprot ID   A0A679GX09
Organism   Metapseudomonas otitidis strain MrB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5639363..5651489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS26105 (PtoMrB4_52010) - 5641005..5642672 (-) 1668 WP_172434849.1 SPOR domain-containing protein -
  PotMrB4_RS26110 (PtoMrB4_52020) aroB 5642683..5643786 (-) 1104 WP_142013730.1 3-dehydroquinate synthase -
  PotMrB4_RS26115 (PtoMrB4_52030) aroK 5643839..5644357 (-) 519 WP_074973697.1 shikimate kinase AroK -
  PotMrB4_RS26120 (PtoMrB4_52040) pilQ 5644363..5646489 (-) 2127 WP_172434850.1 type IV pilus secretin PilQ Machinery gene
  PotMrB4_RS26125 (PtoMrB4_52050) pilP 5646544..5647074 (-) 531 WP_172435161.1 type 4a pilus biogenesis lipoprotein PilP -
  PotMrB4_RS26130 (PtoMrB4_52060) pilO 5647074..5647697 (-) 624 WP_142013726.1 type 4a pilus biogenesis protein PilO -
  PotMrB4_RS26135 (PtoMrB4_52070) pilN 5647694..5648266 (-) 573 WP_172434851.1 type 4a pilus biogenesis protein PilN -
  PotMrB4_RS26140 (PtoMrB4_52080) pilM 5648266..5649330 (-) 1065 WP_074973682.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 708 a.a.        Molecular weight: 76289.88 Da        Isoelectric Point: 5.4089

>NTDB_id=79075 PotMrB4_RS26120 WP_172434850.1 5644363..5646489(-) (pilQ) [Metapseudomonas otitidis strain MrB4]
MNSSLTRLGLSLLAAMFSPMLLAADLKALDVAALPGGKVELKLGFDGPVVQPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTLVEAKDRTRMIINLSTLVPYSTRVEGNNLYVLVGDAAGAAQQAARTSALVAAPSQPVAPVKAFAPAGKAI
SNIDFQRGEQGEGNVVITLSDPSVSPNIQEQGGKIRLDFSKTQIPESLRVRLDVKDFATPVQFVSASGSSGDASIVIEPA
GFYDYLAYQTENKLTVSIKPLTQEDMEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQ
NVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQISELAPLRRELIQVNYAKAADIAKLFQSVTSGE
PNVTDERGSITVDDRTNSIIAYQTQEKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVRWGGAYSNGNWRNFGK
SGNVGIKDGVAGASCGPFQGQCSLPVTNNSPTPFVDMGVSNSTSGIGIGFISSHAILDLQLSAMEKTGNGEIVSQPKVVT
SDKETAKILKGAEIPYQEASSSGATTTTFKEAALSLEVTPQITPDNRIIMEVKVNKDAPDFANQLNGVPPINKNEVNAKV
LVSDGETIVIGGVFSNTQTKSVDKVPFLGDLPFVGRVFRRDIVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2127 bp        

>NTDB_id=79075 PotMrB4_RS26120 WP_172434850.1 5644363..5646489(-) (pilQ) [Metapseudomonas otitidis strain MrB4]
ATGAACAGCTCCCTAACGCGCCTTGGACTTTCTCTTCTGGCGGCCATGTTTTCACCTATGTTGCTGGCTGCTGACCTCAA
GGCCCTAGACGTCGCGGCCCTGCCGGGCGGAAAGGTCGAGCTGAAACTCGGTTTCGACGGCCCCGTTGTACAGCCGAGAG
GCTACACGATTGAGCAGCCTGCAAGGATCGCATTGGACCTGCCTGGCGTTGCCAACAAACTTGGCAGCAAGAACCGCGAG
CTCGGGGTAGGTAATGCTCGGAGCGTCACTCTTGTCGAGGCAAAAGATCGCACTCGCATGATCATCAACCTGTCGACGTT
GGTGCCTTACAGCACGCGAGTCGAGGGTAACAACCTTTACGTTCTGGTTGGCGACGCTGCCGGCGCTGCTCAACAGGCTG
CACGCACCAGTGCGCTGGTAGCGGCTCCGTCACAGCCTGTCGCACCTGTCAAAGCGTTCGCGCCTGCGGGCAAGGCAATC
AGCAACATTGATTTCCAGAGAGGCGAGCAGGGCGAAGGTAATGTCGTCATTACCCTATCCGACCCCAGTGTGAGTCCCAA
CATCCAGGAGCAGGGTGGAAAGATCCGCCTGGACTTTTCCAAGACTCAAATCCCGGAGTCTCTGCGTGTTCGTCTTGATG
TAAAGGATTTCGCGACCCCGGTCCAGTTCGTCAGTGCCTCTGGCAGCTCGGGTGATGCCAGTATCGTGATCGAGCCGGCG
GGCTTCTACGACTACCTGGCCTACCAGACTGAAAACAAGCTGACTGTCAGTATCAAACCGCTTACCCAAGAAGATATGGA
GAAGCGCAAGACCGAGCGCTTTGCCTATTCCGGCGAAAAGCTCTCGCTCAATTTCCAGGATATTGATGTCCGCTCGGTAC
TGCAGCTGATCGCTGACTTCACCGATCTGAACCTGGTTGCGTCCGATACCGTACAAGGCAACATCACGCTCCGCCTGCAG
AATGTACCTTGGGATCAGGCACTTGACCTCGTCCTGAAAACCAAGGGGTTGGACAAGCGCAAGGTCGGTAACGTGCTGCT
GGTTGCACCGGCGGACGAGATCGCTGCCCGTGAACGCCAAGAACTGGAGTCTCAGAAGCAGATTTCCGAATTGGCTCCGC
TGCGCCGCGAGCTCATACAGGTCAACTACGCCAAAGCTGCTGATATCGCCAAGCTCTTTCAGTCTGTTACTAGTGGTGAG
CCGAATGTTACAGATGAGCGCGGTTCGATAACTGTTGATGATCGGACCAACAGTATTATCGCTTACCAGACTCAAGAGAA
GCTGGATGAATTGCGTCGTATTGTTACTCAGCTTGATATTCCTGTGCGCCAAGTAATGATTGAAGCCCGTATTGTTGAGG
CTAATGTTGATTACGATAAGGCTCTGGGGGTGAGGTGGGGTGGCGCTTACAGTAATGGAAATTGGCGGAATTTCGGGAAG
AGCGGTAATGTTGGGATTAAGGATGGTGTCGCTGGTGCTTCCTGTGGTCCTTTTCAGGGCCAGTGCTCACTGCCTGTTAC
TAATAATAGCCCGACTCCCTTCGTCGATATGGGGGTTTCTAATAGCACCTCTGGCATCGGTATAGGCTTCATTTCCAGTC
ATGCAATCCTCGATTTGCAGCTTTCCGCTATGGAGAAGACCGGTAACGGCGAGATTGTTTCCCAGCCTAAAGTAGTTACC
TCTGACAAGGAGACTGCAAAAATTCTGAAAGGCGCGGAGATTCCATATCAGGAAGCTAGTTCCAGTGGGGCGACTACCAC
GACTTTTAAAGAAGCCGCTCTTTCTCTGGAGGTCACTCCGCAAATTACGCCAGACAATCGGATCATCATGGAGGTTAAGG
TCAATAAGGATGCTCCGGACTTTGCAAACCAACTCAATGGTGTGCCTCCGATCAATAAGAACGAGGTAAATGCCAAAGTG
TTGGTGAGTGATGGCGAGACAATCGTAATTGGCGGTGTCTTCTCTAATACACAGACTAAATCTGTGGACAAAGTGCCGTT
CCTCGGTGACCTGCCGTTTGTAGGGCGTGTGTTCAGAAGAGACATAGTCCAAGACAAGAAATCCGAGCTGCTGGTATTCC
TGACTCCGCGTATCATGAACAACCAGGCTATCGCTGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

Secretin and TonB N terminus short domain (STN)

(304-350)

AMIN domain (AMIN)

(40-121)

Bacterial type II and III secretion system protein (Secretin)

(543-699)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679GX09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

78.243

100

0.792
  pilQ Acinetobacter baumannii strain A118

41.89

100

0.419
  pilQ Acinetobacter baumannii D1279779

41.89

100

0.419
  comQ Acinetobacter baylyi ADP1

41.88

99.153

0.415
  pilQ Legionella pneumophila strain Lp02

38.733

95.904

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.733

95.904

0.371

Multiple sequence alignment