Detailed information NTG0079076

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PotMrB4_RS26140
• Genome accession: NZ_AP022642
• Coordinates: 5648266..5649330 (-)
• Length: 354 a.a.
• NCBI ID: WP_074973682.1
• Uniprot ID: -
• Organism: Metapseudomonas otitidis strain MrB4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5643266..5654330

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PotMrB4_RS26115 (PtoMrB4_52030)	aroK	5643839..5644357 (-)	519	WP_074973697.1	shikimate kinase AroK	-
PotMrB4_RS26120 (PtoMrB4_52040)	pilQ	5644363..5646489 (-)	2127	WP_172434850.1	type IV pilus secretin PilQ	Machinery gene
PotMrB4_RS26125 (PtoMrB4_52050)	pilP	5646544..5647074 (-)	531	WP_172435161.1	type 4a pilus biogenesis lipoprotein PilP	-
PotMrB4_RS26130 (PtoMrB4_52060)	pilO	5647074..5647697 (-)	624	WP_142013726.1	type 4a pilus biogenesis protein PilO	-
PotMrB4_RS26135 (PtoMrB4_52070)	pilN	5647694..5648266 (-)	573	WP_172434851.1	type 4a pilus biogenesis protein PilN	-
PotMrB4_RS26140 (PtoMrB4_52080)	pilM	5648266..5649330 (-)	1065	WP_074973682.1	pilus assembly protein PilM	Machinery gene
PotMrB4_RS26145 (PtoMrB4_52090)	-	5649704..5651935 (+)	2232	WP_394354048.1	penicillin-binding protein 1A	-
PotMrB4_RS26150 (PtoMrB4_52100)	-	5652041..5653309 (-)	1269	WP_172434852.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37729.05 Da
• Isoelectric Point: 4.3980

>NTDB_id=79076 PotMrB4_RS26140 WP_074973682.1 5648266..5649330(-) (pilM) [Metapseudomonas otitidis strain MrB4]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKAKAGSKLVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNPERMDVLLAACRRENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLASQLGNGSEDLTVAIVDIGATMTTLSVLNNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=79076 PotMrB4_RS26140 WP_074973682.1 5648266..5649330(-) (pilM) [Metapseudomonas otitidis strain MrB4]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATTCGCTGCTGGGGATCGACATAAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGTGGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCGCCAAATGCTGTCGTCGAAAAGAACA
TTGCTGAGCTGGAAGGCGTCGGCCAGGCACTGTCGCGTGTGGTCGCCAAGGCGAAGGCAGGCTCCAAGCTGGTAGCCGTT
GCTGTGGCGGGTTCGGCCGTCATCACCAAGACGATCGAGATGGATGCGGGGTTGTCCGATGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCTGATCAGTACATTCCTTATCCCCTTGAAGAAGTAGCCATCGATTTCGAGGTTCAGGGCGTTTCTGCAC
GAAACCCCGAGCGAATGGATGTGCTGCTGGCAGCCTGCCGTCGCGAGAACGTTGAAGTGCGCGAGGCAGCGCTTGCTCTC
GCCGGGCTCACCGCGAAAGTCGTGGATGTCGAGGCCTATGCGCTTGAGCGCGCCTATGGGCTCCTTGCTTCCCAGCTGGG
GAATGGATCGGAGGACCTGACGGTTGCGATCGTGGACATCGGCGCGACGATGACCACCCTGAGCGTCTTGAATAATGGTC
GCACCATATACAGTCGCGAGCAGTTGTTCGGAGGTCGCCAGTTGACCGAAGAAATTCAGCGCCGCTATGGCCTGTCTGTC
GAGGAAGCGGGTTTGGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAGGC
AGTTGTCCAGCAAGTCTCGCGGTCGCTGCAGTTCTTTTTCGCAGCGGGTCAGTACAACGATGTCGACTACATCCTCCTGG
CCGGCGGTACAGCCTCTATTCCCGATTTAGATCGCCTGATCCAGCAGAAGATCGGAACGCCGACGCTGGTCGCAAACCCC
TTCGCCGACATGGCACTGAGCGGCAAAGTCAACGCAGGTGCGCTGGCAAGTGATGCGCCGGCCTTGATGATCGCTTGTGG
CTTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.78	100	0.568
comM	Acinetobacter nosocomialis M2	56.78	100	0.568
comM	Acinetobacter baylyi ADP1	53.955	100	0.54
pilM	Legionella pneumophila strain ERS1305867	48.305	100	0.483