Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP65_RS29030 Genome accession   NZ_CP118137
Coordinates   6454153..6455370 (+) Length   405 a.a.
NCBI ID   WP_124325536.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain NCCB 47033     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6449153..6460370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP65_RS29015 (PUP65_29015) - 6449609..6452524 (+) 2916 WP_197723015.1 DUF748 domain-containing protein -
  PUP65_RS29020 (PUP65_29020) - 6452727..6453083 (-) 357 WP_124325535.1 BON domain-containing protein -
  PUP65_RS29025 (PUP65_29025) - 6453373..6453789 (-) 417 WP_025807491.1 pilin -
  PUP65_RS29030 (PUP65_29030) pilC 6454153..6455370 (+) 1218 WP_124325536.1 type II secretion system F family protein Machinery gene
  PUP65_RS29035 (PUP65_29035) pilD 6455373..6456245 (+) 873 WP_063433051.1 prepilin peptidase Machinery gene
  PUP65_RS29040 (PUP65_29040) coaE 6456242..6456865 (+) 624 WP_124325537.1 dephospho-CoA kinase -
  PUP65_RS29045 (PUP65_29045) yacG 6456862..6457065 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP65_RS29050 (PUP65_29050) - 6457070..6457285 (-) 216 WP_025807496.1 hypothetical protein -
  PUP65_RS29055 (PUP65_29055) - 6457351..6458040 (-) 690 WP_124325538.1 energy-coupling factor ABC transporter permease -
  PUP65_RS29060 (PUP65_29060) - 6458037..6458504 (-) 468 WP_102677068.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44634.47 Da        Isoelectric Point: 10.4288

>NTDB_id=790346 PUP65_RS29030 WP_124325536.1 6454153..6455370(+) (pilC) [Pseudomonas chlororaphis strain NCCB 47033]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHNPSLIRAQLRKQGISPVRVRKKSRSLFQRTPPIRARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMAELKQDIAAGSSFTTALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGGLLLSVFGLR
HAHRHSPRFRQWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPSMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790346 PUP65_RS29030 WP_124325536.1 6454153..6455370(+) (pilC) [Pseudomonas chlororaphis strain NCCB 47033]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATAACCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGTCAGGGTCAGGAAGAAATCCC
GTTCGCTGTTCCAGCGCACGCCCCCGATCAGGGCCCGCGACATCAGCTTGTTCACCCGGCAGCTGGCGACCCTGATCAAG
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAACTGATGGCCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCACAGCATTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACGCGCATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGTTGGCGGGCGGTCTGTTGCTCAGCGTGTTCGGCCTGCGC
CATGCCCACCGGCACTCGCCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCACTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAATCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACTGGCATG
CAGTTGCATTTTTCCATGCGCGCGTCCGGCATCTTTCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTGGCGATCCACTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.902

100

0.553
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilG Neisseria gonorrhoeae MS11

41.045

99.259

0.407
  pilC Vibrio cholerae strain A1552

41.582

96.79

0.402
  pilG Neisseria meningitidis 44/76-A

40.299

99.259

0.4
  pilC Vibrio campbellii strain DS40M4

38.308

99.259

0.38