NTDB

Overview

Name	comEA/celA/cilE	Type	Machinery gene
Locus tag	PUW52_RS05495	Genome accession	NZ_CP118078
Coordinates	1086676..1087380 (-)	Length	234 a.a.
NCBI ID	WP_024051601.1	Uniprot ID	A0A412PQ70
Organism	Streptococcus anginosus strain VSI16
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1081676..1092380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW52_RS05475 (PUW52_05475)	-	1082106..1082576 (-)	471	WP_024051595.1	8-oxo-dGTP diphosphatase	-
PUW52_RS05480 (PUW52_05480)	-	1082613..1083266 (-)	654	WP_024051596.1	uracil-DNA glycosylase	-
PUW52_RS05485 (PUW52_05485)	-	1083290..1084276 (-)	987	WP_024051597.1	Gfo/Idh/MocA family oxidoreductase	-
PUW52_RS05490 (PUW52_05490)	-	1084773..1086692 (-)	1920	Protein_1038	ComEC/Rec2 family competence protein	-
PUW52_RS05495 (PUW52_05495)	comEA/celA/cilE	1086676..1087380 (-)	705	WP_024051601.1	helix-hairpin-helix domain-containing protein	Machinery gene
PUW52_RS05500 (PUW52_05500)	smpB	1087563..1088030 (-)	468	WP_024051602.1	SsrA-binding protein SmpB	-
PUW52_RS05505 (PUW52_05505)	rnr	1087993..1090332 (-)	2340	WP_274984260.1	ribonuclease R	-
PUW52_RS05510 (PUW52_05510)	secG	1090424..1090657 (-)	234	WP_003024744.1	preprotein translocase subunit SecG	-
PUW52_RS05515 (PUW52_05515)	rpmG	1090697..1090846 (-)	150	WP_003024746.1	50S ribosomal protein L33	-
PUW52_RS05520 (PUW52_05520)	-	1090830..1092035 (-)	1206	WP_180363719.1	multidrug efflux MFS transporter	-

Sequence

Protein

Download Length: 234 a.a. Molecular weight: 24966.91 Da Isoelectric Point: 4.7586

                                >NTDB_id=789851 PUW52_RS05495 WP_024051601.1 1086676..1087380(-) (comEA/celA/cilE) [Streptococcus anginosus strain VSI16]

MLETIIEKMKEYKILIGLSLIGLIIAGFFMINVQSSRQSNVAELAQETVTSSGAESEEISTGTKKNSQKEKAEPQTSSSE

ESEFLTVDVKGAVKNPGIYQLKKTSRINDAIQKAGGLTTDADSKSINLAQKLTDEAVVYVATMGENAASVSSNTGQSSTS

GTSEVASQKGNKVNLNTADLSELQTISGIGQKRAQDILDYREANGKFNSVDDLKNVSGVGAKTLEKLKEYVTVD

Nucleotide

Download Length: 705 bp

                                >NTDB_id=789851 PUW52_RS05495 WP_024051601.1 1086676..1087380(-) (comEA/celA/cilE) [Streptococcus anginosus strain VSI16]

ATGTTAGAGACAATCATTGAAAAGATGAAAGAGTATAAAATTTTGATTGGTTTAAGTTTGATTGGTTTGATAATAGCAGG

ATTTTTTATGATAAATGTTCAATCTAGTAGACAATCAAATGTAGCAGAGCTCGCACAGGAAACAGTTACGAGTTCGGGGG

CAGAATCAGAAGAAATTTCAACTGGAACGAAGAAAAACTCACAGAAAGAAAAAGCAGAGCCTCAAACGAGTTCAAGTGAA

GAATCAGAATTTTTAACCGTAGATGTCAAGGGTGCAGTCAAAAATCCAGGTATTTATCAACTGAAAAAGACTAGCCGTAT

CAATGATGCGATTCAAAAAGCAGGTGGTTTAACGACAGACGCTGACAGCAAGTCTATCAATTTGGCGCAGAAACTAACGG

ATGAAGCTGTTGTTTATGTGGCAACTATGGGTGAGAATGCGGCGAGTGTTTCAAGTAATACAGGACAATCTTCAACGTCA

GGAACTAGTGAAGTCGCATCGCAAAAAGGAAATAAGGTCAATCTAAATACAGCTGATTTATCTGAGCTGCAGACCATTTC

TGGTATTGGTCAAAAACGTGCGCAAGATATTTTAGATTATCGTGAGGCAAACGGGAAATTTAATTCCGTTGATGACTTGA

AAAATGTATCAGGAGTAGGCGCTAAAACACTAGAAAAATTGAAAGAATATGTCACAGTGGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(87-140)

Helix-hairpin-helix motif (HHH_3)

(169-232)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A412PQ70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA/celA/cilE	Streptococcus pneumoniae D39	53.219	99.573	0.53
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	53.219	99.573	0.53
comEA/celA/cilE	Streptococcus pneumoniae R6	53.219	99.573	0.53
comEA/celA/cilE	Streptococcus pneumoniae Rx1	53.219	99.573	0.53
comEA/celA/cilE	Streptococcus mitis NCTC 12261	51.502	99.573	0.513
comEA/celA/cilE	Streptococcus mitis SK321	50.215	99.573	0.5
comEA	Streptococcus thermophilus LMD-9	44.255	100	0.444
comEA	Lactococcus lactis subsp. cremoris KW2	42.06	99.573	0.419

Detailed information NTG0789851