Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   PUW49_RS05230 Genome accession   NZ_CP118054
Coordinates   1039033..1039875 (+) Length   280 a.a.
NCBI ID   WP_024051528.1    Uniprot ID   A0A412PQ29
Organism   Streptococcus anginosus strain VSI37     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1034033..1044875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW49_RS05205 (PUW49_05205) - 1035349..1035840 (+) 492 Protein_988 MBL fold metallo-hydrolase -
  PUW49_RS05210 (PUW49_05210) ylqF 1035960..1036811 (+) 852 WP_024051524.1 ribosome biogenesis GTPase YlqF -
  PUW49_RS05215 (PUW49_05215) - 1036798..1037580 (+) 783 WP_024051525.1 ribonuclease HII -
  PUW49_RS05220 (PUW49_05220) - 1037577..1038403 (+) 827 Protein_991 PRD domain-containing protein -
  PUW49_RS05225 (PUW49_05225) - 1038393..1038950 (+) 558 WP_070815076.1 sugar O-acetyltransferase -
  PUW49_RS05230 (PUW49_05230) dprA/cilB/dalA 1039033..1039875 (+) 843 WP_024051528.1 DNA-processing protein DprA Machinery gene
  PUW49_RS05235 (PUW49_05235) topA 1039961..1042054 (+) 2094 WP_024051529.1 type I DNA topoisomerase -
  PUW49_RS05240 (PUW49_05240) - 1042118..1042615 (+) 498 WP_024051530.1 phosphoribosylanthranilate isomerase -
  PUW49_RS05245 (PUW49_05245) trmFO 1042789..1044123 (+) 1335 WP_274995211.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 31102.62 Da        Isoelectric Point: 5.0589

>NTDB_id=789676 PUW49_RS05230 WP_024051528.1 1039033..1039875(+) (dprA/cilB/dalA) [Streptococcus anginosus strain VSI37]
MDNFDIYKLKEAGLTNQQVINVLSYAEIQEKELSVKDMAVVSECRNPALFIEKYLQLDDDLLCQEFEKFPSFSILDDVYP
WDLSEIYNPPVLLFYKGNLDLLKLPKVAVVGSRNSSRVGSQSVQKIIKELNNELVIVSGLARGIDTSAHMAALQNGGRTI
AVIGTGLDVFYPRANKKLQSYLGEHHLVLSEYSSEAEPLKFHFPERNRIIAGLCRGVIVADAKMRSGSLITCERAMEEGR
DVFAIPGSILDGRSDGCHHLIQEGAKCITSGADVLSEFQF

Nucleotide


Download         Length: 843 bp        

>NTDB_id=789676 PUW49_RS05230 WP_024051528.1 1039033..1039875(+) (dprA/cilB/dalA) [Streptococcus anginosus strain VSI37]
ATGGATAATTTTGATATCTATAAATTAAAAGAAGCTGGCTTGACCAATCAGCAGGTCATCAATGTTCTCTCGTATGCTGA
GATACAAGAAAAAGAATTATCAGTAAAAGATATGGCAGTTGTGTCGGAATGCCGTAATCCAGCTCTTTTCATTGAAAAAT
ATCTGCAGCTAGATGATGATTTGTTATGTCAAGAATTTGAAAAATTTCCGTCTTTTTCTATCTTGGATGATGTGTATCCT
TGGGATTTGAGTGAAATTTATAATCCACCCGTTCTTCTTTTTTATAAAGGTAATTTGGATTTGCTAAAATTACCTAAAGT
AGCTGTTGTAGGAAGTCGGAATAGTAGCCGAGTAGGCAGTCAATCCGTTCAAAAGATTATTAAAGAGCTAAACAATGAAT
TGGTTATCGTGAGTGGTTTAGCACGCGGAATTGATACCTCGGCTCACATGGCAGCATTGCAAAATGGTGGTCGAACAATT
GCAGTTATCGGAACGGGTCTAGACGTTTTTTATCCAAGGGCGAATAAAAAACTCCAATCGTATTTGGGAGAACATCATCT
AGTACTTAGTGAATATAGTTCTGAAGCAGAGCCATTAAAATTTCATTTTCCAGAGCGTAATCGGATTATAGCAGGATTGT
GCAGAGGGGTTATTGTTGCTGATGCCAAAATGCGCTCAGGCAGCCTTATTACTTGTGAGCGAGCAATGGAAGAAGGGCGA
GATGTATTTGCTATTCCAGGGTCCATTTTAGATGGAAGAAGTGATGGCTGTCATCATTTGATTCAGGAGGGAGCGAAATG
CATCACATCAGGGGCTGACGTACTTTCTGAATTCCAATTTTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-60)

DNA recombination-mediator protein A (DNA_processg_A)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PQ29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

75.714

100

0.757
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

75.357

100

0.754
  dprA/cilB/dalA Streptococcus pneumoniae D39

75.357

100

0.754
  dprA/cilB/dalA Streptococcus pneumoniae R6

75.357

100

0.754
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

75.357

100

0.754
  dprA/cilB/dalA Streptococcus mitis SK321

75

100

0.75
  dprA Streptococcus mutans UA159

65

100

0.65
  dprA Lactococcus lactis subsp. cremoris KW2

56.786

100

0.568
  dprA Staphylococcus aureus N315

36.879

100

0.371
  dprA Staphylococcus aureus MW2

36.655

100

0.368
  dprA Bacillus subtilis subsp. subtilis str. 168

45.374

81.071

0.368