Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   PUW71_RS04915 Genome accession   NZ_CP118053
Coordinates   976572..977276 (-) Length   234 a.a.
NCBI ID   WP_024051601.1    Uniprot ID   A0A412PQ70
Organism   Streptococcus anginosus strain VSI40     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 971572..982276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW71_RS04900 (PUW71_04900) - 972102..973088 (-) 987 WP_024051597.1 Gfo/Idh/MocA family oxidoreductase -
  PUW71_RS04905 (PUW71_04905) - 973718..974278 (-) 561 WP_230309747.1 DUF805 domain-containing protein -
  PUW71_RS04910 (PUW71_04910) comEC/celB 974360..976588 (-) 2229 WP_024051600.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PUW71_RS04915 (PUW71_04915) comEA/celA/cilE 976572..977276 (-) 705 WP_024051601.1 helix-hairpin-helix domain-containing protein Machinery gene
  PUW71_RS04920 (PUW71_04920) smpB 977459..977926 (-) 468 WP_024051602.1 SsrA-binding protein SmpB -
  PUW71_RS04925 (PUW71_04925) rnr 977889..980228 (-) 2340 WP_150888697.1 ribonuclease R -
  PUW71_RS04930 (PUW71_04930) secG 980320..980553 (-) 234 WP_003024744.1 preprotein translocase subunit SecG -
  PUW71_RS04935 (PUW71_04935) rpmG 980593..980742 (-) 150 WP_003024746.1 50S ribosomal protein L33 -
  PUW71_RS04940 (PUW71_04940) - 980726..981931 (-) 1206 WP_171021860.1 multidrug efflux MFS transporter -

Sequence

Protein


Download         Length: 234 a.a.        Molecular weight: 24966.91 Da        Isoelectric Point: 4.7586

>NTDB_id=789613 PUW71_RS04915 WP_024051601.1 976572..977276(-) (comEA/celA/cilE) [Streptococcus anginosus strain VSI40]
MLETIIEKMKEYKILIGLSLIGLIIAGFFMINVQSSRQSNVAELAQETVTSSGAESEEISTGTKKNSQKEKAEPQTSSSE
ESEFLTVDVKGAVKNPGIYQLKKTSRINDAIQKAGGLTTDADSKSINLAQKLTDEAVVYVATMGENAASVSSNTGQSSTS
GTSEVASQKGNKVNLNTADLSELQTISGIGQKRAQDILDYREANGKFNSVDDLKNVSGVGAKTLEKLKEYVTVD

Nucleotide


Download         Length: 705 bp        

>NTDB_id=789613 PUW71_RS04915 WP_024051601.1 976572..977276(-) (comEA/celA/cilE) [Streptococcus anginosus strain VSI40]
ATGTTAGAGACAATCATTGAAAAGATGAAAGAGTATAAAATTTTGATTGGTTTAAGTTTGATTGGTTTGATAATAGCAGG
ATTTTTTATGATAAATGTTCAATCTAGTAGACAATCAAATGTAGCAGAGCTCGCACAGGAAACAGTTACGAGTTCGGGGG
CAGAATCAGAAGAAATTTCAACTGGAACGAAGAAAAACTCACAGAAAGAAAAAGCAGAGCCTCAAACGAGTTCAAGTGAA
GAATCAGAATTTTTAACCGTAGATGTCAAGGGTGCAGTCAAAAATCCAGGTATTTATCAACTGAAAAAGACTAGCCGTAT
CAATGATGCGATTCAAAAAGCAGGTGGTTTAACGACAGACGCTGACAGCAAGTCTATCAATTTGGCGCAGAAACTAACGG
ATGAAGCTGTTGTTTATGTGGCAACTATGGGTGAGAATGCGGCGAGTGTTTCAAGTAATACAGGACAATCTTCAACGTCA
GGAACTAGTGAAGTCGCATCGCAAAAAGGAAATAAGGTCAATCTAAATACAGCTGATTTATCTGAGCTGCAGACCATTTC
TGGTATTGGTCAAAAACGTGCGCAAGATATTTTAGATTATCGTGAGGCAAACGGGAAATTTAATTCCGTTGATGACTTGA
AAAATGTATCAGGAGTAGGCGCTAAAACACTAGAAAAATTGAAAGAATATGTCACAGTGGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(87-140)

Helix-hairpin-helix motif (HHH_3)

(169-232)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PQ70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae D39

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus pneumoniae R6

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus pneumoniae Rx1

53.219

99.573

0.53
  comEA/celA/cilE Streptococcus mitis NCTC 12261

51.502

99.573

0.513
  comEA/celA/cilE Streptococcus mitis SK321

50.215

99.573

0.5
  comEA Streptococcus thermophilus LMD-9

44.255

100

0.444
  comEA Lactococcus lactis subsp. cremoris KW2

42.06

99.573

0.419