Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   PUW70_RS02385 Genome accession   NZ_CP118029
Coordinates   454499..455164 (+) Length   221 a.a.
NCBI ID   WP_024052083.1    Uniprot ID   A0A412PNM5
Organism   Streptococcus anginosus strain VSI68     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449499..460164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW70_RS02360 (PUW70_02360) - 450140..450556 (+) 417 WP_024052089.1 hypothetical protein -
  PUW70_RS02365 (PUW70_02365) dcm 450713..451158 (+) 446 Protein_424 DNA (cytosine-5-)-methyltransferase -
  PUW70_RS02370 (PUW70_02370) cysK 451480..452409 (-) 930 WP_024052086.1 cysteine synthase A -
  PUW70_RS02375 (PUW70_02375) - 452509..453144 (-) 636 WP_024052085.1 YigZ family protein -
  PUW70_RS02380 (PUW70_02380) comFA/cflA 453201..454502 (+) 1302 WP_024052084.1 DEAD/DEAH box helicase Machinery gene
  PUW70_RS02385 (PUW70_02385) comFC/cflB 454499..455164 (+) 666 WP_024052083.1 ComF family protein Machinery gene
  PUW70_RS02390 (PUW70_02390) raiA 455242..455784 (+) 543 WP_003025754.1 ribosome-associated translation inhibitor RaiA -
  PUW70_RS02395 (PUW70_02395) rsmD 455972..456520 (+) 549 WP_171021853.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PUW70_RS02400 (PUW70_02400) coaD 456510..457007 (+) 498 WP_021001374.1 pantetheine-phosphate adenylyltransferase -
  PUW70_RS02405 (PUW70_02405) sepM 456988..458043 (+) 1056 WP_024052081.1 SepM family pheromone-processing serine protease Regulator
  PUW70_RS02410 (PUW70_02410) - 458182..458745 (+) 564 WP_024052080.1 YutD-like domain-containing protein -
  PUW70_RS02415 (PUW70_02415) rlmN 458786..459868 (+) 1083 WP_024052079.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 25367.43 Da        Isoelectric Point: 7.9118

>NTDB_id=789233 PUW70_RS02385 WP_024052083.1 454499..455164(+) (comFC/cflB) [Streptococcus anginosus strain VSI68]
MNSCVLCENPLDEKLKFCDLLTLTKPKARACDACFEKFQRISGQHCPKCYRDGASEVCMDCQVWIEKGKEVYHKSCFVYN
EAMKDFFSQYKFQGDYALYSVFAAVLQKELKVYRDYALVPIPVSAEKYQTRGFNQVTALLEGAKLPFKEVLEKYDTIAQS
SKTREERLQSRQCFKLKDNVQLPPKILLIDDIYTTGSTLQLAKEILVEAGVKEIVTFSLAR

Nucleotide


Download         Length: 666 bp        

>NTDB_id=789233 PUW70_RS02385 WP_024052083.1 454499..455164(+) (comFC/cflB) [Streptococcus anginosus strain VSI68]
ATGAATTCATGTGTTTTATGTGAAAATCCACTTGATGAAAAGCTGAAATTCTGTGATTTATTGACCTTGACGAAGCCAAA
AGCAAGGGCTTGTGATGCCTGCTTTGAAAAATTTCAACGCATTTCAGGGCAGCATTGCCCGAAGTGTTATCGTGACGGGG
CAAGTGAAGTCTGCATGGATTGCCAAGTGTGGATAGAAAAAGGAAAAGAAGTTTACCATAAAAGCTGTTTTGTGTATAAT
GAAGCCATGAAAGACTTCTTCAGTCAATATAAATTTCAAGGAGATTATGCCCTTTATTCTGTATTTGCTGCAGTTTTGCA
AAAGGAATTGAAAGTTTACCGTGATTATGCGCTTGTTCCCATTCCGGTAAGTGCCGAAAAGTATCAGACAAGAGGGTTTA
ATCAAGTAACAGCTCTTCTTGAGGGCGCAAAGTTGCCATTTAAGGAAGTTTTAGAAAAGTATGATACAATTGCTCAATCC
AGTAAAACAAGAGAGGAGCGATTGCAAAGCCGGCAGTGTTTTAAATTGAAAGACAACGTTCAACTTCCACCTAAAATCCT
TTTGATAGATGACATTTATACAACAGGCTCAACCCTACAATTAGCCAAGGAAATTTTAGTGGAAGCAGGAGTGAAAGAAA
TTGTGACATTTTCATTAGCCAGATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PNM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus mitis SK321

49.083

98.643

0.484
  comFC/cflB Streptococcus pneumoniae TIGR4

47.706

98.643

0.471
  comFC/cflB Streptococcus mitis NCTC 12261

47.706

98.643

0.471
  comFC/cflB Streptococcus pneumoniae Rx1

47.248

98.643

0.466
  comFC/cflB Streptococcus pneumoniae D39

47.248

98.643

0.466
  comFC/cflB Streptococcus pneumoniae R6

47.248

98.643

0.466
  comFC Bacillus subtilis subsp. subtilis str. 168

35.683

100

0.367