Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   PUW70_RS02380 Genome accession   NZ_CP118029
Coordinates   453201..454502 (+) Length   433 a.a.
NCBI ID   WP_024052084.1    Uniprot ID   A0AAW5TE88
Organism   Streptococcus anginosus strain VSI68     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448201..459502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW70_RS02360 (PUW70_02360) - 450140..450556 (+) 417 WP_024052089.1 hypothetical protein -
  PUW70_RS02365 (PUW70_02365) dcm 450713..451158 (+) 446 Protein_424 DNA (cytosine-5-)-methyltransferase -
  PUW70_RS02370 (PUW70_02370) cysK 451480..452409 (-) 930 WP_024052086.1 cysteine synthase A -
  PUW70_RS02375 (PUW70_02375) - 452509..453144 (-) 636 WP_024052085.1 YigZ family protein -
  PUW70_RS02380 (PUW70_02380) comFA/cflA 453201..454502 (+) 1302 WP_024052084.1 DEAD/DEAH box helicase Machinery gene
  PUW70_RS02385 (PUW70_02385) comFC/cflB 454499..455164 (+) 666 WP_024052083.1 ComF family protein Machinery gene
  PUW70_RS02390 (PUW70_02390) raiA 455242..455784 (+) 543 WP_003025754.1 ribosome-associated translation inhibitor RaiA -
  PUW70_RS02395 (PUW70_02395) rsmD 455972..456520 (+) 549 WP_171021853.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PUW70_RS02400 (PUW70_02400) coaD 456510..457007 (+) 498 WP_021001374.1 pantetheine-phosphate adenylyltransferase -
  PUW70_RS02405 (PUW70_02405) sepM 456988..458043 (+) 1056 WP_024052081.1 SepM family pheromone-processing serine protease Regulator
  PUW70_RS02410 (PUW70_02410) - 458182..458745 (+) 564 WP_024052080.1 YutD-like domain-containing protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49050.81 Da        Isoelectric Point: 8.0447

>NTDB_id=789232 PUW70_RS02380 WP_024052084.1 453201..454502(+) (comFA/cflA) [Streptococcus anginosus strain VSI68]
MTELQDCLGRIFTKNQLSPELQLQAQTLTGMVEEKGRLSCNRCGQAIDKEKHQLPIGAYYCRSCLILGRVRSDEDLYYFP
QEEFPKANVLKWQGKLTEFQAKVSQGLVEAVTKRKDSLVHAVTGAGKTEMIYQVVAQVINQGGAVCLASPRIDVCLELYR
RLKVDFTCDISLLHGESEAYSRSPLVIATTHQLIKFYRAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKVEGTKIFLTATS
TDELDKKVAKGELTRLSLPRRFHGNPLIVPQKIWLADFQKNLGQKKLVPKLRQFIQKQRKTGFPLLIFASEIRKGQELAE
ILQSTFPNEEVDFVASTTENRLEIVEKFRQKEITILVTTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSME
RPTGELIFFHDGTTMAIEKAIKEIQEMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=789232 PUW70_RS02380 WP_024052084.1 453201..454502(+) (comFA/cflA) [Streptococcus anginosus strain VSI68]
ATGACAGAATTACAAGATTGTTTAGGTCGCATTTTTACAAAAAATCAACTGTCACCAGAATTGCAATTGCAAGCACAAAC
CTTAACTGGAATGGTAGAAGAAAAAGGGAGATTAAGCTGTAATCGCTGTGGACAAGCCATTGACAAAGAAAAACACCAAC
TGCCAATCGGTGCTTATTATTGTAGGTCTTGCTTGATCTTAGGAAGAGTTAGGAGTGATGAAGATCTCTACTATTTTCCA
CAGGAAGAATTTCCCAAAGCGAATGTCTTGAAATGGCAAGGAAAGTTGACAGAATTTCAAGCCAAGGTTTCTCAAGGACT
TGTAGAGGCGGTTACCAAACGCAAAGATAGCTTGGTTCACGCAGTCACGGGAGCCGGAAAGACGGAAATGATCTATCAAG
TGGTGGCACAAGTCATCAATCAGGGCGGAGCAGTCTGCTTGGCTAGCCCCAGAATTGATGTCTGCTTAGAACTTTATCGC
AGACTGAAAGTGGATTTTACTTGTGATATTTCACTCCTGCACGGTGAATCAGAAGCATATTCTCGCAGTCCTCTCGTGAT
TGCCACCACACATCAGCTTATCAAATTTTACCGAGCATTTGATCTTCTTATTGTTGATGAGGTAGATGCCTTTCCTTATG
TGGACAATCCGATGCTTTATCATGCAGTTCATCAGGCAGTCAAAGTAGAGGGGACGAAGATTTTCTTAACAGCAACTTCC
ACAGATGAGCTGGATAAAAAAGTGGCTAAAGGAGAATTAACTCGTTTGAGTCTACCCAGACGTTTTCATGGCAATCCTTT
GATTGTTCCGCAAAAAATTTGGTTGGCGGATTTTCAAAAAAATCTTGGTCAAAAGAAGCTAGTTCCCAAGTTAAGGCAGT
TTATTCAAAAGCAGAGAAAAACAGGATTTCCACTTCTTATTTTTGCTTCCGAGATCAGAAAAGGGCAGGAGCTGGCAGAG
ATTCTTCAAAGCACTTTTCCTAATGAAGAAGTTGATTTTGTAGCCTCGACGACTGAAAATCGACTAGAAATTGTAGAGAA
ATTTCGTCAAAAAGAAATCACGATTTTGGTAACGACGACAATTTTGGAGCGTGGGGTGACTTTTCCTTGTGTAGATGTTT
TCGTGGTGGAAGCCAACCACCGTCTGTTTAGTCGCAGCGCTCTGGTACAAATTGCTGGACGCGTTGGTCGCAGTATGGAG
CGACCGACAGGCGAGTTAATCTTTTTTCATGACGGTACGACTATGGCGATAGAAAAAGCGATTAAAGAAATTCAGGAGAT
GAATCAGGAGGCCGGTTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(291-397)

DEAD/DEAH box helicase (DEAD)

(108-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

70.794

98.845

0.7
  comFA/cflA Streptococcus pneumoniae D39

70.794

98.845

0.7
  comFA/cflA Streptococcus pneumoniae R6

70.794

98.845

0.7
  comFA/cflA Streptococcus pneumoniae TIGR4

70.561

98.845

0.697
  comFA/cflA Streptococcus mitis NCTC 12261

70.892

98.383

0.697
  comFA/cflA Streptococcus mitis SK321

70.188

98.383

0.691
  comFA Lactococcus lactis subsp. cremoris KW2

55.276

91.917

0.508
  comFA Latilactobacillus sakei subsp. sakei 23K

40.092

100

0.402
  comFA Bacillus subtilis subsp. subtilis str. 168

40.247

93.533

0.376