Detailed information NTG0788434

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PT966_RS12665
• Genome accession: NZ_CP117945
• Coordinates: 2594641..2595255 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain ZY1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2589641..2600255

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PT966_RS12635 (PT966_12635)	rpsT	2589941..2590207 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PT966_RS12640 (PT966_12640)	holA	2590224..2591265 (-)	1042	Protein_2445	DNA polymerase III subunit delta	-
PT966_RS12645 (PT966_12645)	-	2591305..2591459 (-)	155	Protein_2446	hypothetical protein	-
PT966_RS12650 (PT966_12650)	-	2591500..2591634 (+)	135	WP_003152870.1	YqzM family protein	-
PT966_RS12655 (PT966_12655)	comEC	2591653..2594004 (-)	2352	WP_072588441.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PT966_RS12660 (PT966_12660)	-	2594005..2594574 (-)	570	WP_003152868.1	ComE operon protein 2	-
PT966_RS12665 (PT966_12665)	comEA	2594641..2595255 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
PT966_RS12670 (PT966_12670)	comER	2595314..2596135 (+)	822	WP_014305449.1	late competence protein ComER	-
PT966_RS12675 (PT966_12675)	-	2596204..2596941 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
PT966_RS12680 (PT966_12680)	rsfS	2596938..2597294 (-)	357	WP_003152864.1	ribosome silencing factor	-
PT966_RS12685 (PT966_12685)	yqeK	2597312..2597872 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PT966_RS12690 (PT966_12690)	-	2597862..2598431 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
PT966_RS12695 (PT966_12695)	yhbY	2598442..2598732 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PT966_RS12700 (PT966_12700)	aroE	2598726..2599568 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=788434 PT966_RS12665 WP_041482377.1 2594641..2595255(-) (comEA) [Bacillus velezensis strain ZY1]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=788434 PT966_RS12665 WP_041482377.1 2594641..2595255(-) (comEA) [Bacillus velezensis strain ZY1]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363