Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   PT966_RS12655 Genome accession   NZ_CP117945
Coordinates   2591653..2594004 (-) Length   783 a.a.
NCBI ID   WP_072588441.1    Uniprot ID   -
Organism   Bacillus velezensis strain ZY1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2586653..2599004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT966_RS12620 (PT966_12620) - 2587016..2587354 (-) 339 WP_003152882.1 YqxA family protein -
  PT966_RS12625 (PT966_12625) - 2587371..2588564 (-) 1194 WP_270355506.1 stage II sporulation protein P -
  PT966_RS12630 (PT966_12630) gpr 2588632..2589738 (-) 1107 WP_003152878.1 GPR endopeptidase -
  PT966_RS12635 (PT966_12635) rpsT 2589941..2590207 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  PT966_RS12640 (PT966_12640) holA 2590224..2591265 (-) 1042 Protein_2445 DNA polymerase III subunit delta -
  PT966_RS12645 (PT966_12645) - 2591305..2591459 (-) 155 Protein_2446 hypothetical protein -
  PT966_RS12650 (PT966_12650) - 2591500..2591634 (+) 135 WP_003152870.1 YqzM family protein -
  PT966_RS12655 (PT966_12655) comEC 2591653..2594004 (-) 2352 WP_072588441.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PT966_RS12660 (PT966_12660) - 2594005..2594574 (-) 570 WP_003152868.1 ComE operon protein 2 -
  PT966_RS12665 (PT966_12665) comEA 2594641..2595255 (-) 615 WP_041482377.1 helix-hairpin-helix domain-containing protein Machinery gene
  PT966_RS12670 (PT966_12670) comER 2595314..2596135 (+) 822 WP_014305449.1 late competence protein ComER -
  PT966_RS12675 (PT966_12675) - 2596204..2596941 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  PT966_RS12680 (PT966_12680) rsfS 2596938..2597294 (-) 357 WP_003152864.1 ribosome silencing factor -
  PT966_RS12685 (PT966_12685) yqeK 2597312..2597872 (-) 561 WP_024085627.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PT966_RS12690 (PT966_12690) - 2597862..2598431 (-) 570 WP_014305453.1 nicotinate-nucleotide adenylyltransferase -
  PT966_RS12695 (PT966_12695) yhbY 2598442..2598732 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86610.59 Da        Isoelectric Point: 9.0078

>NTDB_id=788432 PT966_RS12655 WP_072588441.1 2591653..2594004(-) (comEC) [Bacillus velezensis strain ZY1]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVVVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGNLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLASFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFNMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTGEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=788432 PT966_RS12655 WP_072588441.1 2591653..2594004(-) (comEC) [Bacillus velezensis strain ZY1]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTTCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTGTTGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCTATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACGAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAAATCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATCGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGCCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTAATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGGGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.606

98.595

0.558