Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PRJ39_RS05975 Genome accession   NZ_CP117873
Coordinates   1351970..1353697 (-) Length   575 a.a.
NCBI ID   WP_091797848.1    Uniprot ID   A0A1H3LH53
Organism   Lysobacter enzymogenes strain Lj46     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1346970..1358697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRJ39_RS05950 (PRJ39_05720) - 1347880..1348956 (+) 1077 WP_394540070.1 Nudix family hydrolase -
  PRJ39_RS05955 - 1349104..1349157 (-) 54 Protein_1185 DUF6053 domain-containing protein -
  PRJ39_RS05960 (PRJ39_05725) coaE 1349175..1349786 (-) 612 WP_394540071.1 dephospho-CoA kinase -
  PRJ39_RS05965 (PRJ39_05730) - 1349791..1350678 (-) 888 WP_091797843.1 A24 family peptidase -
  PRJ39_RS05970 (PRJ39_05735) pilC 1350698..1351966 (-) 1269 WP_091797845.1 type II secretion system F family protein Machinery gene
  PRJ39_RS05975 (PRJ39_05740) pilB 1351970..1353697 (-) 1728 WP_091797848.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PRJ39_RS05980 (PRJ39_05745) - 1353775..1355481 (-) 1707 WP_394540072.1 phosphoethanolamine transferase -
  PRJ39_RS05985 (PRJ39_05750) - 1355540..1355980 (-) 441 WP_091797853.1 pilin -
  PRJ39_RS05990 (PRJ39_05755) - 1356824..1357279 (-) 456 WP_394540073.1 pilin -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62685.03 Da        Isoelectric Point: 6.3076

>NTDB_id=788150 PRJ39_RS05975 WP_091797848.1 1351970..1353697(-) (pilB) [Lysobacter enzymogenes strain Lj46]
MSVATTANLVGITGIVRRLVLDGALEDARAREALAAATSERKPIAAYLTEHRLVTPNQLAAAHSVEFGVPLFDPSAMDPG
QAAVRMIGEDLIRKHNALPLFKRGNKLFIGLSDPTNTSALDEIKFQTNAAVEAILVDEDLIRRTLDRWLETSDSLGDTVG
DGEGLETLEIGKDDDLSTKGDSGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHSRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQKELF
VGAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEVAAGITLFEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAQKSGVRDLRRSALVKVL
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=788150 PRJ39_RS05975 WP_091797848.1 1351970..1353697(-) (pilB) [Lysobacter enzymogenes strain Lj46]
ATGTCCGTGGCAACGACAGCCAACCTGGTCGGCATCACCGGCATCGTCCGGCGCTTGGTGCTGGACGGCGCCCTGGAAGA
CGCGCGGGCCCGCGAAGCCTTGGCGGCCGCCACCAGCGAACGCAAGCCGATCGCCGCGTATCTCACCGAACACCGCCTGG
TCACGCCGAATCAACTCGCGGCCGCCCACTCGGTCGAGTTCGGCGTGCCGCTGTTCGATCCCAGCGCCATGGATCCGGGT
CAGGCGGCGGTGCGCATGATCGGCGAAGACCTGATCCGCAAACACAACGCCCTGCCGTTGTTCAAGCGCGGCAACAAGCT
CTTCATCGGCCTGTCCGATCCGACCAACACCTCGGCCCTGGACGAGATCAAGTTCCAGACCAACGCCGCCGTCGAGGCGA
TCCTGGTCGACGAAGACCTGATCCGGCGCACGCTCGATCGCTGGCTGGAGACCTCCGACTCGCTCGGCGATACGGTCGGC
GACGGCGAAGGCCTGGAAACGCTGGAGATCGGCAAGGACGACGACCTGTCGACCAAGGGCGACAGCGGCATCGACGCCAA
GGGCGACGACACCCCCGTCGTCAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCGTCCGACATCCACT
TCGAGCCCTACGAAACCGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCCAACAAG
CTGCACTCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCTCCGCCGCCCGCCTGGGCATCGAGAAGCTCGGCTACGAGGACGACCAGAAGGAATTGTTC
GTCGGCGCGGTGGTCAAGCCCTACGGCATGGTCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGAGCAGCGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAGACCGCGGAAATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCCT
CGGTGACCCTGGTGATCGCCCAGCGCCTGGCCCGGCGCCTGCACGACTGCAAGCGCGAGGTCCACCTGCCCGAACACGCG
CTGCTGGCCGAAGGCTTCACCCACGAGGAAGTCGCCGCCGGCATCACCCTGTTCGAACCGGTCGGCTGCCCCGACTGCAC
CGAGGGCTACAAGGGACGTACCGGCATCTACCAGGTCATGCCGATGACCGACGAAATCCAGGCGATCGTCCTCGAAGGCG
GCAACGCGATGCAGATCGCAGCCGCCGCCCAGAAGTCGGGCGTGCGCGACCTGCGCCGCTCGGCGCTGGTCAAGGTGCTC
AACGGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H3LH53

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.613

100

0.56
  pilB Acinetobacter baumannii D1279779

55.634

98.783

0.55
  pilB Legionella pneumophila strain ERS1305867

50.438

99.304

0.501
  pilB Vibrio cholerae strain A1552

48.951

99.478

0.487
  pilF Neisseria gonorrhoeae MS11

46.842

99.13

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

48.669

91.478

0.445
  pilB Vibrio campbellii strain DS40M4

51.335

84.696

0.435
  pilB/pilB1 Synechocystis sp. PCC 6803

37.108

99.826

0.37
  pilF Thermus thermophilus HB27

39.279

91.652

0.36
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

91.652

0.36