Detailed information NTG0788052

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PO845_RS12365
• Genome accession: NZ_CP117857
• Coordinates: 2578500..2579114 (-)
• Length: 204 a.a.
• NCBI ID: WP_061860722.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain PT4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2573500..2584114

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PO845_RS12335 (PO845_12335)	rpsT	2573803..2574069 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PO845_RS12340 (PO845_12340)	holA	2574086..2575127 (-)	1042	Protein_2377	DNA polymerase III subunit delta	-
PO845_RS12345 (PO845_12345)	-	2575167..2575318 (-)	152	Protein_2378	hypothetical protein	-
PO845_RS12350 (PO845_12350)	-	2575359..2575493 (+)	135	WP_003152870.1	YqzM family protein	-
PO845_RS12355 (PO845_12355)	comEC	2575512..2577863 (-)	2352	WP_280955436.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PO845_RS12360 (PO845_12360)	-	2577864..2578433 (-)	570	WP_003152868.1	ComE operon protein 2	-
PO845_RS12365 (PO845_12365)	comEA	2578500..2579114 (-)	615	WP_061860722.1	helix-hairpin-helix domain-containing protein	Machinery gene
PO845_RS12370 (PO845_12370)	comER	2579173..2579994 (+)	822	WP_061860723.1	late competence protein ComER	-
PO845_RS12375 (PO845_12375)	-	2580063..2580800 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
PO845_RS12380 (PO845_12380)	rsfS	2580797..2581153 (-)	357	WP_007408260.1	ribosome silencing factor	-
PO845_RS12385 (PO845_12385)	yqeK	2581171..2581731 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PO845_RS12390 (PO845_12390)	-	2581721..2582290 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
PO845_RS12395 (PO845_12395)	yhbY	2582301..2582591 (-)	291	WP_061860725.1	ribosome assembly RNA-binding protein YhbY	-
PO845_RS12400 (PO845_12400)	aroE	2582585..2583427 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22263.50 Da
• Isoelectric Point: 6.4888

>NTDB_id=788052 PO845_RS12365 WP_061860722.1 2578500..2579114(-) (comEA) [Bacillus velezensis strain PT4]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNINLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=788052 PO845_RS12365 WP_061860722.1 2578500..2579114(-) (comEA) [Bacillus velezensis strain PT4]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATAT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.143	96.078	0.549
comEA	Lactococcus lactis subsp. cremoris KW2	35.586	100	0.387
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363