Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH64_RS02130 Genome accession   NZ_CP117460
Coordinates   473808..475883 (+) Length   691 a.a.
NCBI ID   WP_305479790.1    Uniprot ID   -
Organism   Pseudomonas sp. FP1742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468808..480883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH64_RS02110 (PSH64_02110) comM 471019..472083 (+) 1065 WP_305479788.1 pilus assembly protein PilM Machinery gene
  PSH64_RS02115 (PSH64_02115) - 472083..472646 (+) 564 WP_305479789.1 PilN domain-containing protein -
  PSH64_RS02120 (PSH64_02120) pilO 472647..473270 (+) 624 WP_105340582.1 type 4a pilus biogenesis protein PilO -
  PSH64_RS02125 (PSH64_02125) - 473267..473794 (+) 528 WP_105340581.1 pilus assembly protein PilP -
  PSH64_RS02130 (PSH64_02130) pilQ 473808..475883 (+) 2076 WP_305479790.1 type IV pilus secretin PilQ Machinery gene
  PSH64_RS02135 (PSH64_02135) aroK 475888..476406 (+) 519 WP_019581860.1 shikimate kinase AroK -
  PSH64_RS02140 (PSH64_02140) aroB 476557..477657 (+) 1101 WP_305479791.1 3-dehydroquinate synthase -
  PSH64_RS02145 (PSH64_02145) - 477668..479275 (+) 1608 WP_305479792.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74812.92 Da        Isoelectric Point: 5.7840

>NTDB_id=785688 PSH64_RS02130 WP_305479790.1 473808..475883(+) (pilQ) [Pseudomonas sp. FP1742]
MNRIFSTFGISLWIAFLSPLVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTEQPARIALDLPGVTSQLASKSRD
LGGGNARSATVVEAKDRTRLIISLTQLTPYNARVEGNNLFVVVGQAAGSKASKPIASAPRAATAVPAPARASAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIVVGFTKTQLPERLRVRLDVKDFATPVQFVNASATGDRAIISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKDLDKRKIGNVLLVAPASEIASRERQELESQKQIAELAPLRRELLQVNYAKAKDIAELFKSVTSADA
KTDERGSITVDERTNNIIVYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSAQKGNWNTSGVNG
SSTTAGTPGSTSTNSPFVDLGTSANTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=785688 PSH64_RS02130 WP_305479790.1 473808..475883(+) (pilQ) [Pseudomonas sp. FP1742]
ATGAACAGGATTTTCTCAACCTTCGGTATTTCGCTATGGATAGCGTTTCTGTCGCCGTTGGTACAGGCGGCCAACCTCAA
AGCGCTGGATGTCGCCGCGCTGCCTGGTGACCGTGTCGAACTGAAGTTGTCGTTCGACGGGCCGCCGCCGCAGCCTCATG
GCTATACGACTGAGCAGCCTGCGCGGATTGCGCTGGACCTGCCGGGGGTTACCAGCCAGCTGGCGAGCAAGAGCCGAGAC
CTGGGCGGCGGCAATGCCCGCAGCGCCACCGTGGTGGAAGCCAAGGACCGCACGCGGTTGATCATCAGTCTGACTCAGCT
GACTCCGTATAACGCACGTGTTGAAGGCAACAACCTGTTTGTGGTCGTCGGGCAGGCGGCCGGTAGCAAGGCGTCCAAAC
CGATTGCCAGCGCACCGCGCGCGGCCACTGCGGTACCCGCACCCGCCAGAGCCAGTGCTCCCGTGGGCAAGGCCATCCGT
GGCGTGGATTTCCAGCGTGGCACCCAGGGTGAAGGCAATGTGGTTATCGATTTGTCGGATCCGTCCATTGCCCCGGACAT
CCAGGAGCGCGATGGCAAGATCGTCGTCGGTTTCACCAAGACTCAATTGCCTGAACGGTTGCGCGTACGTCTGGACGTCA
AGGATTTCGCTACCCCGGTGCAGTTCGTCAACGCCAGCGCCACGGGCGACCGGGCCATCATCAGTATCGAGCCCAGCGGC
GCCTTCGATTATTCGACCTATCAGACCGATAACAAACTGACGGTCAGCATCCGCCCGATGACGGTCGATGACTTGCAGAA
ACGCAACGCCGAGCGTTTTGCCTACAGCGGTGAAAAGCTCTCGCTGAACTTTCAGGACATCGATGTGCGCTCGGTGCTGC
AATTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGTATTACGTTGCGACTGCAGAAC
GTGCCGTGGGACCAGGCACTGGACCTGGTGCTGAAAACTAAAGATTTAGACAAGCGCAAGATCGGTAACGTGCTCCTTGT
GGCGCCCGCCTCTGAAATCGCCTCACGTGAGCGGCAAGAGTTGGAGTCACAAAAACAGATTGCGGAACTGGCGCCGCTGC
GTCGTGAGCTGCTGCAGGTCAACTACGCCAAGGCCAAGGACATCGCAGAGCTGTTCAAGTCGGTGACTAGTGCCGACGCA
AAAACCGATGAGCGTGGCTCGATCACTGTTGACGAGCGAACCAATAACATCATTGTCTATCAGACTCAGGATCGCCTCGA
CGAACTGCGTCGCATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCACGCATCGTCGAAGCCAACG
TCGATTACGACAAAAGCCTTGGCGTGCGCTGGGGTGGTTCGGCGCAAAAGGGGAACTGGAACACATCCGGGGTCAACGGC
TCTTCGACCACCGCCGGCACACCGGGCAGTACCAGCACCAACTCACCGTTCGTCGACCTGGGGACCTCGGCCAATACCTC
GGGGATCGGCATCGCATTCATCACCGACAATGTGCTGCTGGACCTTGAGCTGACGGCCATGGAAAAAACCGGTAACGGGG
AAATCGTCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAGATTCCCTACCAG
GAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCTCTGGAAGTGACGCCGCAAATCACCCC
GGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGACGTACCACCGA
TCAAGAAAAACGAGGTCAACGCCAAGGTATTGGTCAACGACGGCGAGACCATCGTCATTGGTGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTCTC
GGAGAAAAAATCCGAGCTGCTGGTGTTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.709

100

0.741
  comQ Acinetobacter baylyi ADP1

41.265

100

0.434
  pilQ Acinetobacter baumannii strain A118

39.309

100

0.428
  pilQ Acinetobacter baumannii D1279779

39.309

100

0.428
  pilQ Legionella pneumophila strain Lp02

38.144

98.263

0.375
  pilQ Legionella pneumophila strain ERS1305867

38.018

97.829

0.372