Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PSH64_RS02110 Genome accession   NZ_CP117460
Coordinates   471019..472083 (+) Length   354 a.a.
NCBI ID   WP_305479788.1    Uniprot ID   -
Organism   Pseudomonas sp. FP1742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466019..477083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH64_RS02100 (PSH64_02100) - 466829..468097 (+) 1269 WP_018929144.1 malic enzyme-like NAD(P)-binding protein -
  PSH64_RS02105 (PSH64_02105) - 468363..470804 (-) 2442 WP_105340585.1 penicillin-binding protein 1A -
  PSH64_RS02110 (PSH64_02110) comM 471019..472083 (+) 1065 WP_305479788.1 pilus assembly protein PilM Machinery gene
  PSH64_RS02115 (PSH64_02115) - 472083..472646 (+) 564 WP_305479789.1 PilN domain-containing protein -
  PSH64_RS02120 (PSH64_02120) pilO 472647..473270 (+) 624 WP_105340582.1 type 4a pilus biogenesis protein PilO -
  PSH64_RS02125 (PSH64_02125) - 473267..473794 (+) 528 WP_105340581.1 pilus assembly protein PilP -
  PSH64_RS02130 (PSH64_02130) pilQ 473808..475883 (+) 2076 WP_305479790.1 type IV pilus secretin PilQ Machinery gene
  PSH64_RS02135 (PSH64_02135) aroK 475888..476406 (+) 519 WP_019581860.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37994.41 Da        Isoelectric Point: 4.7279

>NTDB_id=785687 PSH64_RS02110 WP_305479788.1 471019..472083(+) (comM) [Pseudomonas sp. FP1742]
MLGLFNKKTSALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALARVLLKAKTSLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGASARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQEHLTVAVVDIGATMTTLSVLNNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVNEVLQPFRDALVQQVSRSLQFFFASGQYSSVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSNMTLGSKVNASALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=785687 PSH64_RS02110 WP_305479788.1 471019..472083(+) (comM) [Pseudomonas sp. FP1742]
GTGCTAGGACTCTTCAATAAAAAAACCAGTGCGCTTTTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAGTT
AAGCCGCCAGGGCGACCGTTACCGGGTCGAGGCCTATGCGGTCGAGCCGCTGCCCGCCAGTGCCGTGGTCGAAAAGAATA
TCGCCGAGCTCGAAGGCGTGGGGCAGGCTCTTGCGCGCGTGCTGCTCAAGGCCAAAACCAGCCTCAGGAACGTCGCCGTG
GCTGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGAGATGGAAAATCAGCT
GAAGATCGAGGCCGATCAGTACATCCCTTATCCATTGGATGAAGTCGCGATCGATTTCGAAGTGCAGGGCGCATCGGCGC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAGGTTCGTGAGGCGGCCCTGGCCCTG
GCCGGGTTGACCGCGCGAGTGGTCGATGTCGAAGCCTATGCCCTGGAGCGATCGTTCGGCCTGCTGGCAACCCAGCTGGC
CGCCTCTCAGGAGCATCTGACCGTGGCGGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGAACAATGGGC
GGATCATCTATACCCGCGAACAATTGTTCGGCGGTCGTCAACTGACAGAAGAAATCCAGCGCCGTTATGGCCTGACGGTC
GAGCAGGCGGGGCTGGCGAAGAAGCAGGGCGGTCTGCCGGATGATTACGTCAATGAAGTCTTGCAGCCGTTTCGTGATGC
GTTGGTGCAACAGGTGTCGCGTTCGTTGCAGTTTTTCTTTGCCTCCGGCCAGTACAGCTCGGTCGATCACATCCTCCTTG
CCGGCGGCACCGCCTCGGTCCCCGGGCTGGACCGGTTGATCGAGCAGCGCCTGGGTACACCGACCCAGGTTGCCAATCCA
TTCTCCAACATGACTCTTGGCAGCAAGGTCAACGCCAGTGCCCTGGCCAGCGATGCGCCGGCACTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.153

100

0.492