Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH78_RS24350 Genome accession   NZ_CP117452
Coordinates   5363412..5365484 (-) Length   690 a.a.
NCBI ID   WP_305497350.1    Uniprot ID   -
Organism   Pseudomonas sp. FP198     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5358412..5370484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH78_RS24335 (PSH78_24330) - 5360053..5361639 (-) 1587 WP_305497348.1 AAA family ATPase -
  PSH78_RS24340 (PSH78_24335) aroB 5361650..5362750 (-) 1101 WP_305497349.1 3-dehydroquinate synthase -
  PSH78_RS24345 (PSH78_24340) aroK 5362889..5363407 (-) 519 WP_181290818.1 shikimate kinase AroK -
  PSH78_RS24350 (PSH78_24345) pilQ 5363412..5365484 (-) 2073 WP_305497350.1 type IV pilus secretin PilQ Machinery gene
  PSH78_RS24355 (PSH78_24350) - 5365481..5366026 (-) 546 WP_305497351.1 pilus assembly protein PilP -
  PSH78_RS24360 (PSH78_24355) pilO 5366023..5366646 (-) 624 WP_305497352.1 type 4a pilus biogenesis protein PilO -
  PSH78_RS24365 (PSH78_24360) - 5366643..5367209 (-) 567 WP_305497353.1 PilN domain-containing protein -
  PSH78_RS24370 (PSH78_24365) comM 5367209..5368273 (-) 1065 WP_305497354.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74752.82 Da        Isoelectric Point: 5.3028

>NTDB_id=785491 PSH78_RS24350 WP_305497350.1 5363412..5365484(-) (pilQ) [Pseudomonas sp. FP198]
MNRFFSALGISLWIALLSPVVHGASLKALDVAALPGDRVELKLTFDGPPPKPRGYTTEQPARIALDLQGVTSQLASKSRD
LGSGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNNLFVVVGKGATPRSPTQPARPAAPVPAPARARAPAGKAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILDFARTQLPEPLRVRLDVKDFATPVQFINASAGSDRATISIEPSGTFD
YSTYQTDNKLTVSVRPMTVEDLQKRDTDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSGVTTGD
STTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=785491 PSH78_RS24350 WP_305497350.1 5363412..5365484(-) (pilQ) [Pseudomonas sp. FP198]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGTTGTTGTCGCCGGTGGTTCATGGCGCCAGCCTGAA
AGCGCTGGATGTGGCGGCGTTACCGGGGGACCGGGTCGAGTTGAAGTTGACCTTCGACGGGCCGCCGCCCAAGCCCCGTG
GTTATACCACCGAGCAACCGGCCCGGATTGCGCTTGATCTGCAGGGGGTGACCAGCCAACTGGCCAGCAAGAGCCGTGAC
CTGGGCAGCGGCAATGCCCGCAGCGCCACCGTGGTCGAGGCGGGGGATCGGACCCGGTTGATCATCGGCCTTACGCAACT
GGCGCCCTATGACACGCGCGTAGAGGGCAACAACCTGTTTGTGGTGGTTGGCAAGGGCGCGACCCCGAGGAGCCCAACCC
AACCCGCTCGCCCGGCAGCCCCCGTCCCTGCGCCTGCCAGGGCTCGCGCCCCGGCGGGCAAAGCCATTCGCGGGGTGGAT
TTCCAGCGCGGGACCCAGGGCGAAGGCAATGTGGTGATCGATTTGTCCGATCCGTCGATCGCCCCGGACATCCAGGAGCG
CGAAGGCAAGATCATCCTCGATTTCGCCAGGACCCAGTTGCCCGAGCCGCTGAGGGTGCGCCTGGACGTCAAGGATTTCG
CCACCCCGGTGCAGTTCATCAATGCCAGCGCCGGCTCCGATCGCGCCACCATCAGCATCGAACCCAGCGGAACCTTTGAT
TACTCGACCTACCAGACCGACAACAAGCTGACCGTCAGTGTCCGGCCGATGACCGTCGAGGACCTGCAAAAACGCGATAC
CGATCGAGCCGCCTATAGCGGCGAAAAATTGTCCCTGAACTTCCAGGACATCGAGGTGCGTTCGGTACTGCAGCTGATTG
CCGATTTCACCAATCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGTATCACCTTGCGGTTGCAGAACGTGCCGTGG
GATCAGGCGCTGGACCTTGTGCTCAAGACCAAGGGGCTCGACAAGCGCAAAGTGGGTAACGTGCTGCTGGTGGCGCCGGC
CGACGAGATCGCCGCCCGCGAACGCCAGGAGCTGGAATCGCAGAAGCAGATTGCCGACCTCGCCCCGTTGCGCCGCGAAC
TGCTGCAGGTCAACTACGCGAAGGCGGCGGACATCGCCAAGCTCTTCCAGTCGGTGACCAGTGCCGAGGCCAAGGCCGAT
GAGCGCGGCACGATCACGGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCAGGACCGGCTCGACGAACTGCG
GCGGATCGTGGCGCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCGCGGATCGTCGAGGCCAACGTCGACTACG
ACAAAAGCCTGGGCGTGCGCTGGGGGGGCTCGATACAGAACAAGGGCAACTGGAACTCTTCCGGGGTGACCACCGGTGAT
TCCACCACCATTGGTACGCCAGGCAGCACCAGCACCAACCAGCCATTCGTCGACCTCGGCGCCTCTGGCAATACGTCGGG
GATCGGTATCGCGTTCATCACCGACAACGTCCTGCTCGACCTTGAGCTGACGGCCATGGAGAAAACCGGCAACGGTGAAA
TCGTCTCACAGCCCAAGGTGGTGACCTCCGACAAGGAGACGGCAAAAATCCTCAAGGGCACGGAGATTCCCTACCAGGAG
GCCAGCTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCCTCGTTGTCGCTTGAGGTCACGCCGCAGATAACCCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTCCAGGATGTACCGCCGATCA
AGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGGGGCGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.905

100

0.757
  comQ Acinetobacter baylyi ADP1

41.823

100

0.419
  pilQ Acinetobacter baumannii strain A118

38.784

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.784

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.519

97.826

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.519

97.826

0.377