Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH78_RS04455 Genome accession   NZ_CP117452
Coordinates   947125..948825 (-) Length   566 a.a.
NCBI ID   WP_305498758.1    Uniprot ID   -
Organism   Pseudomonas sp. FP198     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 942125..953825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH78_RS04430 (PSH78_04430) - 943474..944160 (+) 687 WP_305498752.1 energy-coupling factor ABC transporter permease -
  PSH78_RS04435 (PSH78_04435) yacG 944208..944414 (-) 207 WP_305498753.1 DNA gyrase inhibitor YacG -
  PSH78_RS04440 (PSH78_04440) coaE 944411..945034 (-) 624 WP_305498755.1 dephospho-CoA kinase -
  PSH78_RS04445 (PSH78_04445) pilD 945034..945903 (-) 870 WP_305498756.1 A24 family peptidase Machinery gene
  PSH78_RS04450 (PSH78_04450) pilC 945905..947122 (-) 1218 WP_305498757.1 type II secretion system F family protein Machinery gene
  PSH78_RS04455 (PSH78_04455) pilB 947125..948825 (-) 1701 WP_305498758.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH78_RS04460 (PSH78_04460) - 949049..949459 (+) 411 WP_305498760.1 pilin -
  PSH78_RS04465 (PSH78_04465) - 949745..950098 (+) 354 WP_305498762.1 BON domain-containing protein -
  PSH78_RS04470 (PSH78_04470) - 950416..950712 (-) 297 WP_370871108.1 DUF2845 domain-containing protein -
  PSH78_RS04475 (PSH78_04475) - 950715..953648 (-) 2934 WP_305498765.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62752.77 Da        Isoelectric Point: 5.5675

>NTDB_id=785477 PSH78_RS04455 WP_305498758.1 947125..948825(-) (pilB) [Pseudomonas sp. FP198]
MNDIALSGLAKQLVLAELITEQSAQQAYQQAQRNRTPLVSYLVQSKLVQSRQVAEIASEHFGIALLDLNSLDKDNQPAGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMADVDLDG
LDIESIDDRKQDSIAGQDGDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRVRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAHALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCMHCRRAIEIPEETLLKEGFPR
ERIGSFTIYEPVGCEQCNNGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLVKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785477 PSH78_RS04455 WP_305498758.1 947125..948825(-) (pilB) [Pseudomonas sp. FP198]
ATGAATGACATCGCCCTTAGCGGCCTGGCCAAGCAACTGGTGCTGGCCGAACTGATCACTGAACAAAGCGCGCAGCAGGC
CTACCAACAGGCGCAGCGCAATCGCACGCCCCTGGTCAGCTATCTGGTACAGAGCAAGCTCGTCCAGAGCCGCCAGGTGG
CGGAAATCGCCTCGGAGCATTTTGGTATCGCCCTGCTGGACCTCAACAGCCTCGACAAGGACAACCAGCCCGCTGGCCTG
GTCAGCGAGAAACTGGTGCGCCAGCATCACGCCCTGCCCCTGTGGCGGCGTGGCAACAAGCTGTACGTGGGCATTTCCGA
TCCGACCAATCACCAGGCCATCAACGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGATGACA
AGCTCAGCGATGCCATCGAGAAATTCTTCGAGTCCAGCAGCACGGGCCTGGAAGGCATGGCCGACGTCGACCTCGACGGT
CTGGACATCGAGTCGATCGACGACCGCAAGCAGGACTCGATCGCGGGCCAGGACGGCGACGATGCGCCAGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGATGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTATGAAAAGACCTATCGGG
TACGCGTGCGCACCGACGGTATGCTGCGGGAAGTCGCAAAGCCGCCCATCCAACTGGCCAACCGCATTGCCGCGCGCCTC
AAGGTGATGGCGAGCCTGGACATTTCCGAACGGCGCAAGCCCCAGGACGGTCGGCTGAAGATGCGCCTGTCGAAGACCAA
GTCCATCGATTTCCGGGTCAATACCCTGCCCACGCTCTGGGGAGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAAATGGGCATCGACGCCCTGGGCTACGAACCGGAACAAAAAGACCTGTACATGGCCGCCCTCAAGCAACCCCAGGGC
CTGATACTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTGTCGCTGTATACCGGCTTGAACATCCTCAACACCGTGGA
CATCAATATTTCTACCGCCGAGGACCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTGAATCCGCGCCAGG
GGCTGGATTTCGCCCATGCACTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCAGCCCAGACCGGGCACCTGGTGCTGTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGATCAGGCTACAGAACATGGGCATCCCCGGTTTCAACATCGCCACGGCGGTGCACCTGATCATCGCCCAGCGCC
TGGCGCGCAAGTTGTGCATGCATTGCCGCAGAGCCATCGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGTTTCCCACGG
GAACGCATCGGCTCATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGCTACAAGGGTCGCGTGGGGAT
CTATGAAGTGGTCAAGAACACGCCGGAGCTGCAGCGGCTGATCATGGCCGAAGGCAACTCCCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGCTTCAATGACTTGCGCACCTCGGGGCTGGTCAAAGTGATGCAAGGCGTCACCAGTCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.481

100

0.585
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

54.754

100

0.549
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Neisseria gonorrhoeae MS11

47.78

99.47

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.852

98.763

0.364
  pilF Thermus thermophilus HB27

36.042

100

0.36