Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH79_RS23405 Genome accession   NZ_CP117445
Coordinates   5233383..5235083 (+) Length   566 a.a.
NCBI ID   WP_305439845.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2196     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5228383..5240083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH79_RS23385 (PSH79_23380) - 5228597..5231548 (+) 2952 WP_305439841.1 DUF748 domain-containing protein -
  PSH79_RS23390 (PSH79_23385) - 5231548..5231844 (+) 297 WP_305439842.1 DUF2845 domain-containing protein -
  PSH79_RS23395 (PSH79_23390) - 5232071..5232427 (-) 357 WP_103306538.1 BON domain-containing protein -
  PSH79_RS23400 (PSH79_23395) - 5232725..5233156 (-) 432 WP_305439844.1 pilin -
  PSH79_RS23405 (PSH79_23400) pilB 5233383..5235083 (+) 1701 WP_305439845.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH79_RS23410 (PSH79_23405) pilC 5235086..5236303 (+) 1218 WP_305439846.1 type II secretion system F family protein Machinery gene
  PSH79_RS23415 (PSH79_23410) pilD 5236305..5237177 (+) 873 WP_305439847.1 A24 family peptidase Machinery gene
  PSH79_RS23420 (PSH79_23415) coaE 5237174..5237797 (+) 624 WP_305439849.1 dephospho-CoA kinase -
  PSH79_RS23425 (PSH79_23420) yacG 5237794..5237991 (+) 198 WP_305439851.1 DNA gyrase inhibitor YacG -
  PSH79_RS23430 (PSH79_23425) - 5237998..5238213 (-) 216 WP_007959584.1 hypothetical protein -
  PSH79_RS23435 (PSH79_23430) - 5238279..5238968 (-) 690 WP_305439852.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62657.63 Da        Isoelectric Point: 5.9446

>NTDB_id=785293 PSH79_RS23405 WP_305439845.1 5233383..5235083(+) (pilB) [Pseudomonas sp. FP2196]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRQSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEDMADVDLDG
LDVEAIDDNKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLIKEGFPE
DRIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785293 PSH79_RS23405 WP_305439845.1 5233383..5235083(+) (pilB) [Pseudomonas sp. FP2196]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTTACGGAAAAAAGTGCCCAGCAGGC
CTGGCAACAGGCCCAGCGCAATCGACAGTCACTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTGG
CCGAGATCGCCTCGGAGCACTTCGGCATGGCGTTCCTCGACCTCAATTGTCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCTTGTGGCGACGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCGATCAACGACATTCAGTTCAGCACCGGACTGAGCACCGAAGCCATTCTGGTAGAGGACGACA
AGCTTACCGAGGCCATCGAAAAATTCTTCGACACCCATGCCAGCGGTCTCGAAGACATGGCCGACGTCGATCTCGACGGC
CTCGATGTCGAAGCCATCGATGACAACAAACAGGACGCCATCGGCGGACTCGATGCCGACGATGCGCCGGTGGTGCGTTT
CGTTCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCCTCCGACCTGCATTTCGAGCCTTACGAAAAGAACTACCGCG
TGCGGGTGCGCACCGACGGCATTTTGCGCGAGGTAGCCAAACCGCCCATCCAACTGGCCGGGCGAATTGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGACATCTCGGAACGGCGCAAACCGCAGGACGGGCGAATCAAGATGCGCCTGTCGAAGAGCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCGACCTTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGCATCGATGCGCTCGGTTACGAGCCGGCGCAGAAAGATCTGTACATGGCCGCACTCAAGCAGCCTCAAGGC
ATGATTCTGGTCACCGGCCCCACCGGCTCGGGTAAAACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGATCCGGTTGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GACTCGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGGCACTTGGTGCTCTCGACCCTGCACACCAACAGCGCGGCAGA
AACCCTCACCCGCCTCCACAACATGGGCATCCCCGGCTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGAC
TGGCACGCAAGTTGTGCAGCCATTGCAAGAAAGCCATCGAGATCCCCCGCGAAACCCTGATCAAGGAAGGCTTCCCCGAG
GATCGCATCGGCAGTTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAGAACACACCAGACCTGCAACGGCTGATCATGGCCGAGGGCAATTCGCTGGAAATCGACATTCAGA
TGCGCCGTGACGGTTTCGACGACCTGCGCACTTCCGGTCTGCATAAAGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.584

99.823

0.585
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

51.155

99.47

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.47

0.496
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Thermus thermophilus HB27

36.918

98.587

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

93.64

0.362