Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH79_RS02075 Genome accession   NZ_CP117445
Coordinates   457726..459813 (+) Length   695 a.a.
NCBI ID   WP_305441004.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2196     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452726..464813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH79_RS02055 (PSH79_02055) comM 454938..456002 (+) 1065 WP_305440997.1 pilus assembly protein PilM Machinery gene
  PSH79_RS02060 (PSH79_02060) - 456002..456568 (+) 567 WP_305440999.1 PilN domain-containing protein -
  PSH79_RS02065 (PSH79_02065) pilO 456565..457188 (+) 624 WP_305441001.1 type 4a pilus biogenesis protein PilO -
  PSH79_RS02070 (PSH79_02070) - 457185..457712 (+) 528 WP_305441003.1 pilus assembly protein PilP -
  PSH79_RS02075 (PSH79_02075) pilQ 457726..459813 (+) 2088 WP_305441004.1 type IV pilus secretin PilQ Machinery gene
  PSH79_RS02080 (PSH79_02080) aroK 459818..460336 (+) 519 WP_187679606.1 shikimate kinase AroK -
  PSH79_RS02085 (PSH79_02085) aroB 460489..461589 (+) 1101 WP_305441006.1 3-dehydroquinate synthase -
  PSH79_RS02090 (PSH79_02090) - 461600..463192 (+) 1593 WP_305441008.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75421.78 Da        Isoelectric Point: 5.5582

>NTDB_id=785280 PSH79_RS02075 WP_305441004.1 457726..459813(+) (pilQ) [Pseudomonas sp. FP2196]
MNRIFSTLGFSLWIALMSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKHLD
LGRGNARTATVVEAKDRTRLIVNLTQLAPYDTRVEGNNLFVVVGQGAPTTTARPVAVAASRATTAAASAPARAFVPKTKA
IRGVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDKLRVRLDVKDFATPVQFVNAAATGDRAIITVEP
SGTFDYSTYQTDNKLTVSIRPITADDLQKRNADRQAYIGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTIQGGITLRL
QNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSG
VSNGSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=785280 PSH79_RS02075 WP_305441004.1 457726..459813(+) (pilQ) [Pseudomonas sp. FP2196]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCACCGATGGTTCAGGCGGCTAATCTGAA
GGCGCTGGACGTAGCAGCGTTGCCGGGTGACCGTGTCGAGTTGAAGCTGTCATTCGACGGCCCGCCGCCGCAGCCCAAGG
GCTACACCACCGATTCGCCGGCGCGGATCGCGCTGGATTTGCCCGGTGTCGCCAGCCAGTTGGCCAGCAAGCATCTCGAT
CTGGGTAGGGGCAATGCACGCACGGCCACGGTGGTTGAAGCGAAGGATCGGACGCGCCTGATCGTCAATCTCACGCAATT
GGCGCCATATGACACGCGGGTCGAGGGCAACAATCTTTTTGTGGTGGTAGGTCAGGGGGCGCCGACAACCACTGCGCGCC
CGGTTGCTGTGGCGGCGTCCCGTGCGACCACGGCAGCGGCTTCCGCACCCGCCAGGGCGTTCGTGCCAAAAACCAAAGCC
ATTCGCGGCGTGGATTTTCAACGAGGCACGGAGGGGGAGGGTAACGTGGTCATCGACCTGTCCGACCCGACCATTGCTCC
CGATATTCAGGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCCGACAAGCTGCGGGTGCGCCTCG
ACGTGAAGGATTTCGCCACGCCGGTGCAGTTCGTCAACGCCGCCGCCACTGGCGACCGCGCGATCATCACCGTTGAGCCC
AGCGGTACGTTCGATTACTCCACCTACCAGACCGATAACAAACTGACCGTCAGCATCCGGCCGATCACGGCGGATGACCT
GCAAAAACGCAACGCAGATCGACAGGCCTATATCGGTGACAAACTATCACTGAACTTCCAGGACATCGACGTGCGCTCGG
TGCTGCAACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCGAGTGACACGATTCAGGGCGGGATCACTCTGCGTCTG
CAAAATGTGCCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGTT
GCTGGTCGCTCCGGCCGACGAAATCGCCGCCCGCGAACGCCAGGAGCTCGAATCGCAGAAACAGATCGCCGAACTGGCGC
CACTGCGCCGTGAGCTGTTGCAAGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCC
GAGGCCAAAATCGACGAGCGTGGCTCGATTACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACTCAGGATCG
TCTCGATGAACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCACGGATCGTTGAAG
CCAACGTCGATTACGACAAGAGTCTGGGTGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACACCTCCGGG
GTCAGTAACGGCTCATCGACCACCATCGGCACGCCGGGTAGCACCAGCACCAATTCGCCATTCGTCGACATGGGCACTGT
CAACAACACGTCCGGGATTGGTATCGCCTTCATCACTGACAATGTTCTGCTCGACCTTGAGCTGACGGCCATGGAGAAGA
CCGGTAACGGCGAAATCGTCTCGCAACCGAAGGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAG
ATTCCCTATCAGGAAGCCAGCTCCAGCGGCGCGACCTCGGTATCGTTCAAAGAAGCCTCGCTGTCCCTGGAAGTGACACC
GCAGATCACCCCCGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGG
ATGTACCGCCGATCAAGAAGAACGAGGTCAACGCGAAGGTGCTGGTCAATGACGGTGAAACCATCGTGATTGGTGGCGTT
TTCTCAAATACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(305-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.207

100

0.744
  comQ Acinetobacter baylyi ADP1

41.236

100

0.413
  pilQ Acinetobacter baumannii strain A118

37.784

100

0.407
  pilQ Acinetobacter baumannii D1279779

37.784

100

0.407
  pilQ Legionella pneumophila strain Lp02

37.85

97.698

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.41

0.368