Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH83_RS02290 Genome accession   NZ_CP117443
Coordinates   523837..525924 (+) Length   695 a.a.
NCBI ID   WP_305491564.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP2262     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 518837..530924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH83_RS02270 (PSH83_02270) comM 521046..522110 (+) 1065 WP_122565300.1 pilus assembly protein PilM Machinery gene
  PSH83_RS02275 (PSH83_02275) - 522110..522676 (+) 567 WP_122565301.1 PilN domain-containing protein -
  PSH83_RS02280 (PSH83_02280) pilO 522673..523296 (+) 624 WP_122565302.1 type 4a pilus biogenesis protein PilO -
  PSH83_RS02285 (PSH83_02285) - 523293..523823 (+) 531 WP_305469354.1 pilus assembly protein PilP -
  PSH83_RS02290 (PSH83_02290) pilQ 523837..525924 (+) 2088 WP_305491564.1 type IV pilus secretin PilQ Machinery gene
  PSH83_RS02295 (PSH83_02295) aroK 525929..526447 (+) 519 WP_122565305.1 shikimate kinase AroK -
  PSH83_RS02300 (PSH83_02300) aroB 526596..527696 (+) 1101 WP_305469357.1 3-dehydroquinate synthase -
  PSH83_RS02305 (PSH83_02305) - 527707..529296 (+) 1590 WP_122565307.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75136.29 Da        Isoelectric Point: 5.5835

>NTDB_id=785228 PSH83_RS02290 WP_305491564.1 523837..525924(+) (pilQ) [Pseudomonas beijingensis strain FP2262]
MNRIFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLTNRRHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNNLFVVVGKGQTPKNTASKPSAQAPRPAVASAAPARASAPAGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGTSTTIGTPGSTGTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=785228 PSH83_RS02290 WP_305491564.1 523837..525924(+) (pilQ) [Pseudomonas beijingensis strain FP2262]
ATGAACAGGATTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTTGGGGCCAGTCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCACCTTCGACGGGCCGCCGCCAACACCCCGGG
GCTACACCACCGAACAGCCGGCCCGGATTGCCCTGGACCTGCCAGGCGTGACCAATCAACTGACCAACAGGCGTCACGAC
CTGGGCGGGGGCAATGCGCGCAGCGCCACCGTGGTCGAGGCGGGGGATCGCACCCGGTTGATCATCGGCCTGACCCAACT
GGCGCCGTATGACACCCGGGTCGAAGGCAATAACCTGTTCGTGGTGGTTGGCAAGGGCCAGACCCCGAAGAATACTGCTT
CGAAGCCCTCTGCCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCCGCGCGGGCCAGTGCGCCGGCGGGCAAGGCG
ATTCGTGGGGTGGATTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGATTTGTCCGATCCATCCATCGCCCC
GGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAATTGCCCGAGCCACTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCTGGCAGCGACCGGGCGACCATCAGCATCGAGCCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTGGACGACTT
GCAAAAACGTAATGCCGACCGCGCCGCCTACAGCGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAAGTGCGTTCGG
TCCTGCAACTGATTGCCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACAGTGCAGGGTGGCATAACCCTGCGGTTG
CAGAACGTGCCTTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAACGTGCT
GCTGGTGGCCCCGGCCGATGAAATCGCGGCGCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTAGCGC
CGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGTTGTTCCAGTCGGTGACCAGTGCC
GAGGCGAAAGCCGACGAGCGCGGCACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
GCTCGACGAACTGCGCCGGATCGTGGCACAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGGTCGATACAGAACAAGGGCAACTGGAACTCCTCGGGG
GTGACCACTGGCACCTCCACGACCATCGGCACGCCGGGCAGTACCGGCACCAACCAGCCGTTCGTCGACCTGGGTGCCTC
GGGTAATACCTCGGGGATCGGCATTGCGTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACTGCCATGGAAAAAA
CCGGCAACGGCGAAATTGTCTCCCAGCCCAAGGTGGTGACATCTGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAG
ATCCCTTATCAGGAAGCCAGTTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTATCGCTGGAGGTGACCCC
GCAAATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCTGATTACCTGAACAAGGTGCAGG
AAGTGCCACCGATCAAGAAAAACGAAGTCAACGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.007

100

0.751
  pilQ Acinetobacter baumannii strain A118

38.904

100

0.419
  pilQ Acinetobacter baumannii D1279779

38.904

100

0.419
  comQ Acinetobacter baylyi ADP1

41.81

100

0.419
  pilQ Legionella pneumophila strain Lp02

38.144

97.698

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.018

97.266

0.37