Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PSH83_RS02270 Genome accession   NZ_CP117443
Coordinates   521046..522110 (+) Length   354 a.a.
NCBI ID   WP_122565300.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP2262     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516046..527110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH83_RS02265 (PSH83_02265) - 518393..520840 (-) 2448 WP_305491563.1 penicillin-binding protein 1A -
  PSH83_RS02270 (PSH83_02270) comM 521046..522110 (+) 1065 WP_122565300.1 pilus assembly protein PilM Machinery gene
  PSH83_RS02275 (PSH83_02275) - 522110..522676 (+) 567 WP_122565301.1 PilN domain-containing protein -
  PSH83_RS02280 (PSH83_02280) pilO 522673..523296 (+) 624 WP_122565302.1 type 4a pilus biogenesis protein PilO -
  PSH83_RS02285 (PSH83_02285) - 523293..523823 (+) 531 WP_305469354.1 pilus assembly protein PilP -
  PSH83_RS02290 (PSH83_02290) pilQ 523837..525924 (+) 2088 WP_305491564.1 type IV pilus secretin PilQ Machinery gene
  PSH83_RS02295 (PSH83_02295) aroK 525929..526447 (+) 519 WP_122565305.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38107.75 Da        Isoelectric Point: 4.9070

>NTDB_id=785227 PSH83_RS02270 WP_122565300.1 521046..522110(+) (comM) [Pseudomonas beijingensis strain FP2262]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVIEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKIIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTDEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=785227 PSH83_RS02270 WP_122565300.1 521046..522110(+) (comM) [Pseudomonas beijingensis strain FP2262]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCACTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTCGAGTT
GAGTCGCCAGGGCGAGCGATACCGCGTCGAGTCCTACGCGGTCGAACCTTTGCCGGCCAATGCAGTGATCGAGAAGAACA
TCGCCGAACTCGAAGGGGTGGGCCAGGCGTTGTCGCGGCTGCTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCCGTGGCGGGCTCGGCGGTGATCACCAAGATCATCGAGATGGACGCGGGCCTCTCCGACGACGACATGGAGAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTCGAAGTGGTGGGCGTTTCGCCGC
GCAGCGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTTCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGAGCCTTTGGCCTGCTCGCCACGCAACTGGC
CGCGTCCCAGGAGCGGCTGACGGTGGCGGTCATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGGCGCCAGTTGACCGATGAAATTCAGCGCCGCTACGGCCTGACACCC
GAACAGGCCGGCCTGGCAAAGAAGCAGGGTGGGTTGCCGGACGATTATCTCAGCGAAGTGCTGCAACCTTTTCGCGAGGC
CCTGGTGCAGCAGGTCTCGCGGTCCCTGCAATTTTTCTTCGCCTCGGGCCAGTACAGCGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCGTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGTGCCCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.367

100

0.554
  pilM Acinetobacter baumannii D1279779

55.367

100

0.554
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475