NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	PO908_RS01275	Genome accession	NZ_CP117042
Coordinates	228302..228739 (+)	Length	145 a.a.
NCBI ID	WP_041783987.1	Uniprot ID	A0AAP6EPH2
Organism	Streptococcus anginosus strain CGMH_KH1
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 223302..233739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PO908_RS01240 (PO908_01240)	glnA	223476..224822 (+)	1347	WP_003027006.1	type I glutamate--ammonia ligase	-
PO908_RS01245 (PO908_01245)	-	225011..225376 (+)	366	WP_003027003.1	DUF1033 family protein	-
PO908_RS01250 (PO908_01250)	comYA	225446..226387 (+)	942	WP_394468008.1	competence type IV pilus ATPase ComGA	Machinery gene
PO908_RS01255 (PO908_01255)	comYB	226329..227351 (+)	1023	WP_041783985.1	competence type IV pilus assembly protein ComGB	Machinery gene
PO908_RS01260 (PO908_01260)	comYC	227348..227665 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PO908_RS01265 (PO908_01265)	comYD	227625..228053 (+)	429	WP_021001232.1	competence type IV pilus minor pilin ComGD	Machinery gene
PO908_RS01270 (PO908_01270)	comGE/cglE	228025..228318 (+)	294	WP_021001233.1	competence type IV pilus minor pilin ComGE	Machinery gene
PO908_RS01275 (PO908_01275)	comGF/cglF	228302..228739 (+)	438	WP_041783987.1	competence type IV pilus minor pilin ComGF	Machinery gene
PO908_RS01280 (PO908_01280)	comGG	228720..229046 (+)	327	WP_021001234.1	competence type IV pilus minor pilin ComGG	-
PO908_RS01285 (PO908_01285)	comYH	229118..230071 (+)	954	WP_021001235.1	class I SAM-dependent methyltransferase	Machinery gene
PO908_RS01290 (PO908_01290)	-	230118..231317 (+)	1200	WP_021001236.1	acetate kinase	-
PO908_RS01295 (PO908_01295)	glmS	231669..233483 (+)	1815	WP_003026983.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

Download Length: 145 a.a. Molecular weight: 16769.29 Da Isoelectric Point: 10.0941

                                >NTDB_id=782220 PO908_RS01275 WP_041783987.1 228302..228739(+) (comGF/cglF) [Streptococcus anginosus strain CGMH_KH1]

MFKANKVKAFTLLEALIALFVISGSVLLFQSMTRLLASEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD

GKVIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIHVRLTFRRGLEREFVYRVETES

Nucleotide

Download Length: 438 bp

                                >NTDB_id=782220 PO908_RS01275 WP_041783987.1 228302..228739(+) (comGF/cglF) [Streptococcus anginosus strain CGMH_KH1]

GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGCCCTCTTTGTCATTAGTGGAAGTGTGTT

GCTGTTTCAATCTATGACGCGCCTTCTAGCTTCTGAAGTCCAAGCTCGTCAACACAGTGAACAACGAGAATGGCTTTTGT

TTGCAGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC

GGCAAGGTGATTGCATTTGGAAAGTCAAGTGGACAGGATTTCAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT

GGTTTATGATGTAAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATGTTAGACTGACGTTTAGGCGAGGATTGG

AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-134)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae R6	66.901	97.931	0.655
comGF/cglF	Streptococcus pneumoniae TIGR4	66.901	97.931	0.655
comGF/cglF	Streptococcus pneumoniae Rx1	66.901	97.931	0.655
comGF/cglF	Streptococcus pneumoniae D39	66.901	97.931	0.655
comGF/cglF	Streptococcus mitis NCTC 12261	67.143	96.552	0.648
comGF/cglF	Streptococcus mitis SK321	67.143	96.552	0.648
comYF	Streptococcus mutans UA159	45.185	93.103	0.421
comYF	Streptococcus mutans UA140	45.185	93.103	0.421
comGF	Lactococcus lactis subsp. cremoris KW2	42.222	93.103	0.393

Detailed information NTG0782220